Comparison of histopathological and flow cytometric parameters in prediction of prognosis in gastric cancer

Wyatt, J I; Quirke, Philip; Ward, Daniel; Clayden, A D; Dixon, M. F.; Johnston, D.; Bird, C. C.

doi:10.1002/path.1711580305

Cited by 34 publications

(27 citation statements)

References 23 publications

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“…Furthermore, even in multivariate analysis, DNA ploidy has been shown to be a prognostic factor [51,59,60]. However, some reports have demonstrated that DNA aneuploidy might be associated with a significantly shorter survival only in patients with intestinal-type tumors [61] or only in patients with diffuse-type cancer [62]. Taken together, this evidence strongly supports the hypothesis that aneuploidy is associated with the prognosis of gastric cancer.…”

Section: Gastric Cancer and Dna Aneuploidysupporting

confidence: 64%

Clinical aspect and molecular mechanism of DNA aneuploidy in gastric cancers

et al. 2012

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The biological characteristics of cancers depend mostly on genetic alterations in the cancer cells of individuals. Gastric cancers show a high frequency of DNA aneuploidy, a phenotype of chromosomal instability. Compared to diploid tumors, gastric carcinomas with aneuploidy have been shown to have high proliferative activity and high metastatic or invasive potential; these characteristics lead to a poor prognosis. It has been suggested that an abnormal spindle assembly checkpoint is involved in DNA aneuploidy, but the underlying mechanism is still unclear. This review, in order to determine whether gastric carcinomas that display aneuploidy are associated with a poorer prognosis than diploid tumors, and to discuss the biological mechanisms that induce aneuploidy, summarizes the results of studies on DNA ploidy in gastric cancer published in the English literature. Analysis of DNA ploidy in gastric cancer may provide clinically useful information from diagnostic, therapeutic, and prognostic standpoints. Further investigations may be needed to clarify the relationship between chromosome instability and DNA ploidy.

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Section: Gastric Cancer and Dna Aneuploidysupporting

confidence: 64%

Clinical aspect and molecular mechanism of DNA aneuploidy in gastric cancers

et al. 2012

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“…In stomach cancer, there have been numerous studies correlating DNA flow cytometry with patient prognosis. Several studies showed that patients with stomach cancer with an aneuploid tumour cell population or with a higher proliferative activity had worse prognoses (Tosi et al, 1988;Bronzo et al, 1989;Korenaga et al, 1989;Nanus et al, 1989;Wyatt et al, 1989;Yonemura et al, 1990;Baretton et al, 1991;Kimura and Yonemura, 1991;Johnson Jr et al, 1993;Kakeji et al, 1993;Rugge et al, 1994;Yonemura et al, 1994;D'Agnano et al, 1995;Flyger et al, 1995;Ikeguchi et al, 1995;Sakusabe et al, 1996;Victorzon et al, 1996). Other studies failed to show such correlation (Sasaki et al, 1989;Filipe et al, 1991;Sarbia et al, 1996).…”

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confidence: 99%

Prognostic value of DNA flow cytometry in stomach cancer: a 5-year prospective study

et al. 1999

Br J Cancer

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SummaryThe role of DNA flow cytometry in the prediction of prognosis for patients with stomach cancer remains to be defined. Thus we studied prospectively the role of DNA flow cytometry as a prognosis indicator in stomach cancer patients in a high-incidence area. Between November 1990 and December 1992, primary stomach cancer tissues were obtained from the surgical specimens from 217 patients (148 male, 69 female). DNA flow cytometric analyses of DNA ploidy and S-phase fraction were performed and the results were correlated with patient survival. The median age of the patients was 55 years (range 24-78). Aneuploid cell population was found in 114 of 217 samples (53%). Tumour S-phase fraction was obtained in 96 of 103 diploid tumours (93%) and 61 of 114 aneuploid tumours (54%). After median follow-up of 66.1 months, the patients with tumours with an S-phase fraction over 17% had significantly worse survival rates than patients with tumours with S-phase fractions of lower than 8% or 8-17% (45% vs 59% and 63% of patients surviving, P = 0.007). Tumour ploidy status did not correlate with patient survival. Multivariate analyses showed that the TNM stage remained the most important prognostic indicator. The tumour S-phase fraction was also an independent prognostic indicator (relative risk 2.300, 95% CI, 1.252-4.223). Tumour S-phase fraction obtained by DNA flow cytometry is an independent prognostic indicator for the survival of the patients with stomach cancer.

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“…They suggested that other factors besides DNA ploidy are responsible for tumor aggressiveness. Filipe et al [7] and Wyatt et al [8] reviewed 116 and 76 cases of gastric carcinoma, respectively, and also failed to find a relationship between DNA ploidy and patient survival.…”

Section: Resultsmentioning

confidence: 95%