Comparison of hydrogen production in microalgae under autotrophic and mixotrophic media

Abstract

Duangjan K., Nakkhunthod W., Pekkoh J., Pumas C., 2017: Comparison of hydrogen production in microalgae under autotrophic and mixotrophic media. -Bot.Lith., 23(2): 169-177.Hydrogen is an alternative source of energy of considerable interest, because it is environmentally friendly. Biological hydrogen production processes involving green microalgae are of significant interest. However, until present only few microalgae genera have been studied and almost all of those studies have focused only on cultivation using mixotrophic or heterotrophic media, which are expensive, and can be easily contaminated. This study aimed to compare the potential of biohydrogen production from novel green microalgae under autotrophic and mixotrophic media. A total of ninety strains of six orders of green microalgae were investigated for their capabilities of hydrogen production. The results showed that eleven novel hydrogen-producing microalgae genera were found. The hydrogen production in each order was influenced by the medium. Moreover, several strains presented notable levels of autotrophic hydrogen production and performed at over twice of the mixotrophic medium. These results should be supportive information for the selection and cultivation of hydrogen-producing microalgae in further studies.

A Review on Mathematical Modeling of Different Biological Methods of Hydrogen Production

“…Table 2 shows that Selenastrum bibraianum AARL G052 in a JM-S medium and sulfurdeprived TAP growth medium produced 0.28 µmol/mg Chl-a/h and 0.71 µmol/mg Chla/h, respectively, when other conditions remained unchanged [46]. A genetically modified Cyanothece ∆hupL mutant and Cyanothece C ∆hupL mutant produced 84.0 ± 21.6 µmol H 2 /mgChl-a/day and 2224.8 ± 434.4 µmol H 2 /mgChl-a/day.…”

Microorganism‐Assisted Biohydrogen Production and Bioreactors

“…Green algae strains that are reviewed by researchers since 1930 for biohydrogen production are Chlorella, Chlamydomonas, Anabaena, Platymonas sp., Tetraspora, Coelastrella , and Scenedesmus 34, 63, 95, 105. Other potential algae species for hydrogen production are Pandorina, Ulothrix, Staurastrum, Closterium, Cosmarium, Dimorphococcus, Euastrum , and Selenastrum 63.…”

Photoautotrophic Production of Hydrogen in Carteria Crucifera AARL G045 Co-Cultured with Bacterial Flora

“…The results are supported by several investigations suggesting that photoautotrophic condition may promote hydrogen production in various green microalgae. However, it is also important to note that each microalga has a different suitable medium for hydrogen production (SonG et al, 2011;HwanG et al, 2014;Duangjan et al, 2017;PholChan et al, 2017). Concerning the economic and upscale production, the direct biophotolysis is more advantageous as the media does not require any organic carbon source thereby obtaining a low-cost medium (Duangjan et al, 2017;Nagarajan et al, 2017).…”

“…However, the commercial disadvantages of those trophic conditions are higher cost from organic carbon source and obligate axenic operation. Thus, autotrophic conditions have been considered for hydrogen production of microalgal strains (HwanG et al, 2014;Duangjan et al, 2017).…”

“…According to the previous study of Duangjan et al (2017), several green algae have been described for the capacity of hydrogen production under autotrophic conditions. Among these, Carteria crucifera AARL G045 is a promising strain because of its rapid growth rate, greater hydrogen production and easy to be harvested because of large cell size (Duangjan et al, 2017). The genus Carteria is a unicellular green microalga, which is classified in the family Chlamydomonadaceae, the same as Chlamydomonas, a model microorganism for biohydrogen production.…”