Comparison of immune parameters in cows with normal and prolonged involution time of uterus

Levkut, M.; Pistl, J.; Révajová, Viera; Choma, J; Levkutová, M.; David, V.

doi:10.17221/5835-vetmed

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…Postpartum peripheral lymphocytes are reduced in animals with delayed uterine involution (Levkut et al, 2002). Specifically, cows with delayed uterine involution have reduced CD2 + , CD4 + , CD8 + and B-cells in peripheral blood.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Bromfield

Watt

Iacovides

2018

Veterinary Immunology and Immunopathology

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Bacterial contamination of the uterus following calving is ubiquitous in the dairy cow, 40% of cows develop postpartum uterine infection, including metritis. While predisposing factors like twinning and dystocia are associated with metritis, it is unclear why some cows remain healthy following calving and others develop uterine infection, negatively impacting animal health, milk production and economic return. Here, we profiled peripheral blood mononuclear cells of cows before calving and during postpartum metritis. We hypothesized that peripheral blood mononuclear cell function and proportions would be altered during the prepartum period in cows that develop postpartum metritis. Using flow cytometry we observed reduced proportions of peripheral CD3/CD4, CD4/CD62L, FOXP3 and CD21 populations from -10 to 40 days relative to calving associated with metritis, while the proportion of peripheral CD3/CD4 lymphocytes were specifically reduced in the prepartum period before the onset of metritis. Peripheral blood mononuclear cells from cows with metritis had a perturbed capacity to secrete IL-1β or IFNγ in response to in vitro stimulus; cells collected during the prepartum period from cows that would go on to develop metritis failed to increase IL-1β secretion in response to stimulation, while IFNγ secretion was altered at calving and postpartum in cows with metritis compared to healthy herd mates. No effect of metritis was observed in the capacity of cows to mount a humoral immune response to antigen administered on the day of calving. The studies discussed here suggest that while minor changes to the prepartum immune system are observed in cows that develop metritis, changes observed in the postpartum period are more prevalent and likely a consequences of disease and not causative. Future studies to modulate the prepartum immune system may help to limit postpartum metritis.

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Section: Discussionmentioning

confidence: 99%

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Bromfield

Watt

Iacovides

2018

Veterinary Immunology and Immunopathology

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show abstract

Section: Discussionmentioning

confidence: 99%

“…This idea seems to be supported by previous data demonstrating a decrease in PMN function 5 and potential modifications of both the innate and adaptive immune systems in cows with endometritis. 12,13 Cytokines play an important role in a wide range of reproductive-related processes via regulation of a complex metabolic network. 56 The activation of pathways leading to biosynthesis of IL-2 and activity of IL-1 in cows with SCE indicates an activation of both innate and adaptive immune systems.…”

Section: Termmentioning

confidence: 99%

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Postpartal Subclinical Endometritis Alters Transcriptome Profiles in Liver and Adipose Tissue of Dairy Cows

Akbar

Cardoso

Meier

et al. 2014

Bioinform Biol Insights

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Transcriptome alterations in liver and adipose tissue of cows with subclinical endometritis (SCE) at 29 d postpartum were evaluated. Bioinformatics analysis was performed using the Dynamic Impact Approach by means of KEGG and DAVID databases. Milk production, blood metabolites (non-esterified fatty acids, magnesium), and disease biomarkers (albumin, aspartate aminotransferase) did not differ greatly between healthy and SCE cows. In liver tissue of cows with SCE, alterations in gene expression revealed an activation of complement and coagulation cascade, steroid hormone biosynthesis, apoptosis, inflammation, oxidative stress, MAPK signaling, and the formation of fibrinogen complex. Bioinformatics analysis also revealed an inhibition of vitamin B3 and B6 metabolism with SCE. In adipose, the most activated pathways by SCE were nicotinate and nicotinamide metabolism, long-chain fatty acid transport, oxidative phosphorylation, inflammation, T cell and B cell receptor signaling, and mTOR signaling. Results indicate that SCE in dairy cattle during early lactation induces molecular alterations in liver and adipose tissue indicative of immune activation and cellular stress.

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“…In cows with prolonged uterine involution time, a significant decrease in the absolute number of lymphocytes and CD2+, CD4+ and CD8+ T cells on days 7, 14 and 21 after parturition was detected (Levkut et al, 2002).…”

Section: Introductionmentioning

confidence: 96%

Immunohistochemistry Study in Normally Calved Postpartum Dairy Cows

Alagar¹,

Selvaraju²,

Napolean³

2017

Int.J.Curr.Microbiol.App.Sci

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Comparison of immune parameters in cows with normal and prolonged involution time of uterus

Cited by 9 publications

References 12 publications

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis

Postpartal Subclinical Endometritis Alters Transcriptome Profiles in Liver and Adipose Tissue of Dairy Cows

Immunohistochemistry Study in Normally Calved Postpartum Dairy Cows

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