Comparison of immunofluorescence and enzyme immunoassay for detection of measles-specific immunoglobulin M antibody

Rossier, E; Miller, H.; McCulloch, B. Jan; Sullivan, Lee; Ward, Kathy

doi:10.1128/jcm.29.5.1069-1071.1991

Cited by 29 publications

(10 citation statements)

References 5 publications

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“…The measles-IgM detection sensitivity of this ELISA was 96.6% for vaccinated subjects and 100% for nonvaccinated subjects, both of which had complement fixation test-confirmed measles. Similar sensitivities were reported previously when 17 serum samples from nonvaccinated subjects were tested (13) and when this ELISA kit was compared with the immunofluorescence assay by using 72 immunofluorescence assay-positive serum samples (10). Interfering IgG, which might have caused false-negative results because of competition with IgM for attachment sites in the ELISA wells, was eliminated prior to measles-IgM testing.…”

Section: Discussionsupporting

confidence: 60%

Performance and reliability of the Enzygnost measles enzyme-linked immuno-sorbent assay for detection of measles virus-specific immunoglobulin M antibody during a large measles epidemic

Ozanne

d'Halewyn

1992

J Clin Microbiol

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Evaluation of the Enzygnost Measles Enzyme-Linked Immuno-Sorbent Assay kit (Behring) performance to detect specific immunoglobulin M (IgM) was carried out with 3,297 single serum samples and 898 paired serum samples collected during a measles epidemic (10,184 reported cases) in Quebec, Canada. Anti-measles IgM and IgG were detected by using the Enzygnost kit with the appropriate conjugates. Complement-fixing (CF) antibody (Ab) titers were assessed by the laboratory branch complement fixation micromethod. The Centers for Disease Control's clinical measles case definition was used. A modification of the manufacturer's optical density interpretation algorithm was introduced to allow for equivocal results, in addition to positive and negative ones. These three categories differed as to their association with a significant increase in CF Ab titer and the time between the onset of symptoms and phlebotomy. The IgM positivity rate for complement fixation-confirmed measles cases was 96.6% for vaccinated subjects and 100% for nonvaccinated subjects. The daily percentage of IgM seropositivity that was detected for subjects who became IgM positive within 30 days increased gradually from 40 to 90% for sera taken 1 to 7 days after the onset of symptoms, and it plateaued at 100% for sera taken 16 to 30 days after the onset of symptoms. IgM seropositivity was strongly associated with IgG seroconversion, CF Ab titer increase, and clinical measles (P less than 0.0001). Reproducibility was 100% for nonreactive sera and 99.1% for reactive sera. In conclusion, the Enzygnost Measles Enzyme-Linked Immuno-Sorbent Assay kit performed adequately to confirm measles virus infection during this epidemic. A second serum sample should be tested when an early-acute-phase serum sample is IgM negative.

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Section: Discussionsupporting

confidence: 60%

Performance and reliability of the Enzygnost measles enzyme-linked immuno-sorbent assay for detection of measles virus-specific immunoglobulin M antibody during a large measles epidemic

Ozanne

d'Halewyn

1992

J Clin Microbiol

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“…Maximal average levels of specific IgM were reached between days 5 and 10 after the onset of rash. This is in agreement with earlier studies with total IgM isolated by sucrose gradient and tested by HI (day 10) (6) or by IgM Enzygnost (days 5 to 10) (28,32).…”

Section: Discussionsupporting

confidence: 93%

“…However, the overall sensitivity strongly depends on the number of sera collected during the later time points. In some studies only 0, 8, 5, or 4.5% of the samples were collected after day 15 (32), day 19 (23), day 25 (26), or day 30 (1), respectively. In contrast, 37% of the samples in our study were drawn after day 20 and 25% of the samples were collected after day 30.…”

Section: Discussionmentioning

confidence: 99%

“…Diagnosis of measles may be confirmed by virus isolation, by the demonstration of a significant increase in specific immunoglobulin G (IgG) titers, or by the detection of anti-measles virus (MV) IgM antibodies by using radioimmunoassays (2,18,37), enzyme-linked immunosorbent assays (ELISAs) (27,34), and direct or indirect fluorescence-antibody techniques (20,24). MV IgM appears at the same time as rash (12,21,34) and can be detected 3 days after the onset of rash in most individuals (29,32). IgM peaks on days 7 to 10 and wanes within weeks (28).…”

mentioning

confidence: 99%

“…Since IgM is transient, the demonstration of spe-cific IgM corresponds to a recent primary measles infection (14,21). A single serum specimen collected at the appropriate time (14) is now generally accepted to be sufficient to diagnose measles (10,12,14,17,26,32,34,37).…”

mentioning

confidence: 99%

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Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

et al. 1998

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Recombinant hemagglutinin (H) of the measles virus (MV) expressed in a mammalian high-expression system based on the Semliki Forest virus replicon was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG in patients with acute-phase measles. One hundred twelve serum specimens from 70 patients with measles were analyzed. Case definition was based on a commercial IgM ELISA that utilizes MV-infected cells (MV-ELISA) (Enzygnost; Behring Diagnostics); the clinical criteria of the Centers for Disease Control and Prevention (Atlanta, Ga.); and/or the increase in hemagglutinin test titers, neutralization test titers, and levels of MV-specific IgG whenever paired sera were available. The initial time courses of the IgM signal after the onset of rash are similar in the H- and MV-ELISAs. On days 0 to 19, both ELISAs detected IgM in 67 of 68 (98.5%) sera. Average maximal levels of IgM seem to persist, however, about 10 days longer in the MV-ELISA (up to day 25) than in the H-ELISA (day 15). From days 20 to 29 and 30 to 59, the H-ELISA detected only 64.3 (9 of 14) and 19.2% (5 of 26), respectively, of sera that were IgM positive by MV-ELISA. At least up to day 30, the performance of the H-ELISA seemed to be similar to that reported for commercial ELISAs based on whole MV. Our results demonstrate that MV H-specific IgM can be used to diagnose most measles cases from a single serum specimen collected within 19 days after the onset of rash and that the recombinant protein used in this study is suitable for this purpose.

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IgM antibody capture haemadherence test (MACHAT) for the detection of measles specific IgM

Siqueira

Ferro

Cohen

et al. 1994

Journal of Virological Methods

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Comparison of immunofluorescence and enzyme immunoassay for detection of measles-specific immunoglobulin M antibody

Cited by 29 publications

References 5 publications

Performance and reliability of the Enzygnost measles enzyme-linked immuno-sorbent assay for detection of measles virus-specific immunoglobulin M antibody during a large measles epidemic

Performance and reliability of the Enzygnost measles enzyme-linked immuno-sorbent assay for detection of measles virus-specific immunoglobulin M antibody during a large measles epidemic

Evaluation of Hemagglutinin Protein-Specific Immunoglobulin M for Diagnosis of Measles by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Protein Produced in a High-Efficiency Mammalian Expression System

IgM antibody capture haemadherence test (MACHAT) for the detection of measles specific IgM

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