Comparison of immunofluorescence, particle agglutination, and enzyme immunoassays for detection of human T-cell leukemia virus type I antibody in African sera

Verdier, Mireille; Denis, F.; Léonard, Guy; Sangaré, A.; Patillaud, S; Prince-David, Mireille; Essex, Max

doi:10.1128/jcm.28.9.1988-1993.1990

Cited by 11 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serological testing for detection of antibodies against HTLV-1 is the first line of testing. Options for serological testing include ELISA, particle agglutination, Western blots and chemiluminescence assays (25,26). Of note, ELISA and particle agglutination tests are seen as screening tests with western blot, chemiluminescence assays and molecular testing being confirmatory.…”

Section: Diagnostic Tests and Screeningmentioning

confidence: 99%

A Review of the Prevention of Mother-to-Child Transmission of Human T-Cell Lymphotrophic Virus Type 1 (HTLV-1) With a Proposed Management Algorithm

et al. 2022

View full text Add to dashboard Cite

Human T cell lymphotropic virus type 1 (HTLV-1) is a human retrovirus that is endemic in a number of regions across the world. There are an estimated 5–10 million people infected worldwide. Japan is currently the only country with a national antenatal screening programme in place. HTLV-1 is primarily transmitted sexually in adulthood, however it can be transmitted from mother-to-child perinatally. This can occur transplacentally, during the birth process or via breastmilk. If HTLV-1 is transmitted perinatally then the lifetime risk of adult T cell leukemia/lymphoma rises from 5 to 20%, therefore prevention of mother-to-child transmission of HTLV-1 is a public health priority. There are reliable immunological and molecular tests available for HTLV-1 diagnosis during pregnancy and screening should be considered on a country by country basis. Further research on best management is needed particularly for pregnancies in women with high HTLV-1 viral load. A first step would be to establish an international registry of cases and to monitor outcomes for neonates and mothers. We have summarized key risk factors for mother-to-child transmission of HTLV-1 and subsequently propose a pragmatic guideline for management of mothers and infants in pregnancy and the perinatal period to reduce the risk of transmission. This is clinically relevant in order to reduce mother-to-child transmission of HTLV-1 and it's complications.

show abstract

Section: Diagnostic Tests and Screeningmentioning

confidence: 99%

A Review of the Prevention of Mother-to-Child Transmission of Human T-Cell Lymphotrophic Virus Type 1 (HTLV-1) With a Proposed Management Algorithm

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Therefore, 46 of 533 samples were categorized as indeterminate and 487 samples were categorized as seronegative by WHO criteria. Although PA and the ELISA are used for serological mass screening, false-positive results have been observed, especially with sera obtained from pregnant women and patients with autoimmune diseases (21,33). To confirm seropositivity, we used WB because of its facility.…”

Section: Characteristics Of Anti-htlv-i Gp46 Monoclonal Antibod-mentioning

confidence: 99%

Improvement of simultaneous detection of antibodies to Gag and envelope antigens of human T-lymphotropic virus type I by western immunoblot assay

et al. 1992

View full text Add to dashboard Cite

To determine seropositivity for human T-lymphotropic virus type I (HTLV-I), we attempted to improve the detection system that uses antibody to HTLV-I Env in Western immunoblotting (WB) by adding an envelope glycoprotein (gp46) purified from the culture fluid of HTLV-I-producing cells by immunoaffinity chromatography and gel chromatography. In this VB, 177 of 179 serum samples showing seropositivity in an indirect immunofluorescence assay showed positive reactions to the gp46 envelope antigen as well as to p19, p24, and p53 Gag antigens. The remaining two samples showed negative reactions to p24. False-positive results were not found for 533 indirect immunofluorescence assay-negative serum samples, although one band to p19 or p24 was observed in 46 of the 533 samples. These 46 samples did not react to p53 and gp46, suggesting that these samples belonged to the indeterminate group in accordance with the criteria proposed by the World Health Organization. Therefore, this improved WB can be used for the confirmation of seropositivity.

show abstract

Detection of HTLV‐I and HTLV‐II infection in Africans using type‐specific envelope peptides

Goubau

Desmyter

Swanson

et al. 1993

Journal of Medical Virology

View full text Add to dashboard Cite

Antibodies to HTLV were determined in 4,630 black African individuals from Zaire, Ghana and South Africa; 185 (4%) were confirmed as seropositive. Seroprevalance was 0.2% in a group of South African women, 0.9% among Ghanaian refugees in Belgium and from less than 1% to over 15% in various sites and populations in Zaire. With the use of HTLV-I and HTLV-II type-specific envelope peptides, 93% of confirmed HTLV seropositives were classified as HTLV-I. Five persons from the Haut Zaire region had HTLV-II serological reactivities, suggesting the presence of HTLV-II or a related retrovirus in central Africa. A cluster of HTLV-I-like indeterminate western blot patterns lacking anti-p24 antibody was found in Bas Zaire.

show abstract

Comparison of immunofluorescence, particle agglutination, and enzyme immunoassays for detection of human T-cell leukemia virus type I antibody in African sera

Cited by 11 publications

References 10 publications

A Review of the Prevention of Mother-to-Child Transmission of Human T-Cell Lymphotrophic Virus Type 1 (HTLV-1) With a Proposed Management Algorithm

A Review of the Prevention of Mother-to-Child Transmission of Human T-Cell Lymphotrophic Virus Type 1 (HTLV-1) With a Proposed Management Algorithm

Improvement of simultaneous detection of antibodies to Gag and envelope antigens of human T-lymphotropic virus type I by western immunoblot assay

Detection of HTLV‐I and HTLV‐II infection in Africans using type‐specific envelope peptides

Contact Info

Product

Resources

About