Comparison of immunological and microbiological characteristics in children and the elderly with or without dental caries

Yang, Ying Ming; Li, Yuhong; Lin, Yingsong; Du, Minquan; Zhang, Ping; Fan, Mingwen

doi:10.1111/eos.12172

Cited by 21 publications

(25 citation statements)

References 34 publications

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“…Detection of genes in S. mutans, R. dentocariosa , several Actinomyces species and S. salivarius in coronal caries compared with caries-free sites is consistent with other reports [5,29–32]. Detection of genes to these species fit with the expanded ecological plaque hypotheses that proposes blooming of Actinomyces and non-mutans streptococci in early stages of dental caries [17].…”

Section: Discussionsupporting

confidence: 87%

Functional profiles of coronal and dentin caries in children

Kressirer

Chen

Harriman³

et al. 2018

Journal of Oral Microbiology

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Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition.Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compared with health.Design: Samples from children, caries-free (CF: n = 4) or with coronal (CC: n = 5) or dentin (DC: n = 5) caries were examined for gene expression potential. Functional profiling was performed using HUMAnN2 (HMP Unified Metabolic Analysis Network).Results: There was increased gene expression diversity in DC compared with CC and CF. Genes in CF included alcohol dehydrogenase from Neisseria sicca, methylenetetrahydrofolate reductase from Streptococcus sanguinis and choline kinase from streptococci. Genes in CC mapped mainly to Streptococcus mutans. Arginine deiminase in DC mapped to S. sanguinis and Actinomyces naeslundii. Glycerol kinase genes mapped to S. sanguinis in all groups whereas glycerol kinase in DC were from Rothia, Prevotella and streptococci. Uracil-DNA glycosylase in DC mapped to Prevotella denticola and Actinomyces. Repressor LexA in DC mapped to Scardovia wiggsiae, Dialister invisus and Veillonella parvula.Conclusions: Functional profiling revealed enzyme activities in both caries and caries-free communities and clarified marked differences between coronal and dentin caries in bacterial composition and potential gene expression.

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Section: Discussionsupporting

confidence: 87%

Functional profiles of coronal and dentin caries in children

Kressirer

Chen

Harriman³

et al. 2018

Journal of Oral Microbiology

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“…Serum IgG isotypes were determined according to methods described in a previous study, 2 and the levels of specific antibodies against GLU were determined using an ELISA with Maxisorp microtiter plates (Greiner Bio-one, Frickenhausen, Germany) coated with GLU as described previously. 28…”

Section: Immunization Sample Collection and Antibody Analysismentioning

confidence: 99%

RETRACTED ARTICLE: Self-assembling anticaries mucosal vaccine containing ferritin cage nanostructure and glucan-binding region ofS. mutansglucosyltransferase effectively prevents caries formation in rodents

Cao

et al. 2017

Human Vaccines & Immunotherapeutics

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Anticaries protein vaccines that induce a mucosal immune response are not effective. Therefore, development of effective and convenient anticaries vaccines is a priority of dental research. Here we generated self-assembling nanoparticles by linking the glucan-binding region of Streptococcus mutans glucosyltransferase (GLU) to the N-terminal domain of ferritin to determine whether these novel nanoparticles enhanced the immunogenicity of an anticaries protein vaccine against GLU in rodents. We constructed the expression plasmid pET28a-GLU-FTH and purified the proteins from bacteria using size-exclusion chromatography. BALB/c mice were used to evaluate the ability of GLU-ferritin (GLU-FTH) nanoparticles to induce GLU-specific mucosal and systemic responses. The protective efficiency of GLU-FTH nanoparticles was compared with that of GLU alone or a mixture of GLU and poly(I:C) after administering an intranasal infusion to Wistar rats. The phagocytosis and maturation of dendritic cells (DCs) exposed in vitro to the nanoparticles were assessed using flow cytometry. The GLU-FTH nanoparticle vaccine elicited significantly higher levels of GLU-specific antibodies compared with GLU or a mixture of GLU and poly(I:C). Immunization with GLU-FTH achieved lower caries scores compared with those of the other vaccines. Administration of GLU-FTH nanoparticles enhanced phagocytosis by DCs and their maturation. Thus, self-assembling GLU-FTH is a highly effective anticaries mucosal vaccine that enhanced antibody production and inhibited S. mutans infection in rodents.

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“…Selecting the participants from different kindergarten in Baghdad from al-karkh sector, after receiving the child guardian approval, ensuring no systemic disease, for the selected cases their BMI was measured (height and weight to determine BMI) [22] to ensure they are in the same wanted range (15)(16)(17)(18)(19)(20), then the teeth of selected children were checked and their dmf was recorded according to the criteria of (WHO, 1987) [23].…”

Section: Methodsmentioning

confidence: 99%

Salivary Immunoglobulin A in Relation to the Viable Count of Mutans Streptococci in Children with Early Childhood Caries

2017

IJSR

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Introduction: Early childhood caries develops in children due to the unsuitable nocturnal feeding habit, with the presence of mutans streptococci as the caries initiating factor, competing with immunological constituent in saliva, the presence of salivary immunoglobulin A (SIgA) as an antimicrobial peptide could have a defensive role to decrease the amount of the viable count of mutans streptococci. Aims of study: this research was done to find the relations between the variables: viable count of mutans streptococci, SIgA level, dental caries index. Material and Methods: categorizing the seventy-five children (4-5) years into 3 groups (twenty-five each) mild, moderate and severe. dmfs, dmft measured according to WHO (1987), salivary Immunoglobulin A level measured by ELISA kit, viable count of mutans streptococci measured after culturing it in (Mitis Salivaris Bacitracine) agar and simple identification method. Result: the statistical analysis revealed that there was significant correlation in the viable count of MS, dmfs and dmft between (mild and moderate) and between (mild and severe). The Salivary Immunoglobulin A (mean value) showed an increase from the mild to severe as the (dmft, dmfs) increase, in which a significant correlation had been found between (mild and severe) in boys and in the total groups, a significant correlation between the viable count of MS and SIgA in the total groups. Conclusion: as the carious (tooth, surface) number increased the viable count of mutans streptococci increased indicating its role in the ECC, SIgA increased as the MS count increased indicating the induction of the immune system to produce salivary immunoglobuline A. that it has no role on the mutans streptococci.

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Comparison of immunological and microbiological characteristics in children and the elderly with or without dental caries

Cited by 21 publications

References 34 publications

Functional profiles of coronal and dentin caries in children

Functional profiles of coronal and dentin caries in children

RETRACTED ARTICLE: Self-assembling anticaries mucosal vaccine containing ferritin cage nanostructure and glucan-binding region ofS. mutansglucosyltransferase effectively prevents caries formation in rodents

Salivary Immunoglobulin A in Relation to the Viable Count of Mutans Streptococci in Children with Early Childhood Caries

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