Functional profiles of coronal and dentin caries in children

Kressirer, Christine A.; Chen, Tsute; Harriman, Kristie Lake; Frias-Lopez, Jorge; Dewhirst, Floyd E.; Tavares, Mary

doi:10.1080/20002297.2018.1495976

Cited by 36 publications

(42 citation statements)

References 44 publications

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“…Our previous study tracked the changes of microbiota over time from the healthy status to caries occurrence and caries progression, thus developed a model for caries prediction and suggested a panel of Prevotella species that may be closely related to caries disease 21,22 . Prevotella's association with caries was also verified by many other studies 24,25 . These indicated that the overexpressed collagenases for proteolytic metabolism in Prevotella species may lead to the progression of dental caries 23 .…”

Section: Discussionsupporting

confidence: 65%

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Quantitative Analysis of Salivary Oral Bacteria Associated with Severe Early Childhood Caries and Construction of Caries Assessment Model

Zhang

Sun

Zhu

et al. 2020

Sci Rep

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To construct a saliva-based caries risk assessment model, saliva samples from 176 severe early childhood caries (S-ECC) children and 178 healthy (H) children were screened by real-time PCR-based quantification of the selected species, including Streptococcus mutans, Prevotella pallens, Prevotella denticola and Lactobacillus fermentum. Host factors including caries status, dmft indices, age, gender, and geographic origin were assessed in their influence on abundance of the targeted species, which revealed host caries status as the dominant factor, followed by dmft indices (both P < 0.01). Moreover, levels of S. mutans and P. denticola in the S-ECC group were significantly higher than those in the healthy group (P < 0.001 for S. mutans and p < 0.01 for P. denticola). Interestingly, the co-occurrence network of these targeted species in the S-ECC group differed from that from the healthy group. Finally, based on the combined change pattern of S. mutans and P. pallens, we constructed an S-ECC diagnosis model with an accuracy of 72%. This saliva-based caries diagnosis model is of potential value for circumstances where sampling dental plague is difficult. Early childhood caries (ECC) is defined as the presence of one or more decayed, missing, or filled tooth surfaces in the primary dentition in children of 71 months or younger 1. Severe early childhood caries (S-ECC), an extraordinary form of ECC, is defined as the presence of decayed, missing, or filled score surfaces of either ≥4 (age 3 years), ≥5 (age 4 years), or ≥6 (age 5 years) 2. In USA, 23% of children between the ages of 2 and 5 are affected by ECC 3. In China, fresh reports from the Fourth National Oral Health Survey showed that over 70% of 5-year-old children carry dental caries in primary teeth 4. Unfortunately, childhood caries are wide-ranging, rapid-progressing and irreversible 5. Besides, severe caries can cause pulpal infection, as well as varieties of adverse physical and psychological effects, thus it affects children's development while posing a substantial economic burden on both families and society 6-8. Therefore, preventive measures and early diagnosis of ECC or S-ECC are of vital clinical and social importance. Many studies have shown that caries is a multifactorial disease 9,10 and pathogenic bacteria are the main cause of disease occurrence and progression 11. Streptococcus mutans (S. mutans) has been considered as a cariogenic bacterial agent in children 12-15 , due to its aciduric and acidogenic properties 16. Apart from this, Lactobacillus spp. was also linked to caries development and progression 16-18. Positive associations between certain Lactobacillus spp. (especially Lactobacillus fermentum) and the hard tissue changes were revealed in the process of caries progression 19,20. In addition, our past pyrosequencing of oral and plaque microbiota unveiled Prevotella spp's close relationship with caries, in both cross-sectional and longitudinal studies 21,22. Specifically, we proposed a caries

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Section: Discussionsupporting

confidence: 65%

“…A number of studies have also reported strong association between several Prevotella spp. (in particular P. denticola) and caries status as well [23][24][25] .…”

mentioning

confidence: 99%

Quantitative Analysis of Salivary Oral Bacteria Associated with Severe Early Childhood Caries and Construction of Caries Assessment Model

Zhang

Sun

Zhu

et al. 2020

Sci Rep

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“…For instance, similar levels of relative abundance in samples from caries‐free and coronal caries‐active children were revealed for the genera Neisseria , Veillonella , Granulicatella , Gemella , as well as the species Veillonella parvula , S . sanguinis , Streptococcus cristatus (Kressirer et al, ). Recognition of the oral core microbiome would be useful for a deeper understanding of the caries pathogenesis and providing insight into microbial interactions in polymicrobial diseases (Shiba et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Caries is developed when pH decreases due to the fermentation by acid‐producing bacteria of readily available carbohydrates to organic acids, which consequently leads to overgrowth of acidotolerant bacteria and suppression of acid‐sensitive species (Bowen, ; McLean et al, ; Zero et al, ). Metatranscriptome analysis of the caries has confirmed “ecologic plaque hypothesis”; genes with increased expression have been found to contribute the metabolic changes favourable for development of acidogenic and acid‐tolerant microbial communities (Kressirer et al, ).…”

Section: Introductionmentioning

confidence: 97%

Oral microbiomes in children with asthma and dental caries

et al. 2019

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Objective Recently, a significant association between dental caries and the severity of bronchial asthma in children has been revealed. This finding indicates a possible relationship between the oral microbiome and the pathogenesis of asthma. The purpose of our study was to estimate differences in the dental plaque microbiota of asthmatic children with and without dental caries by 16S rDNA sequencing. Material and methods Dental plaque samples were obtained with a spoon excavator from the occlusal surface of one deciduous tooth (the second mandibular left molar in caries‐free children and the most affected tooth in caries‐affected children). Total DNA was extracted from dental plaque. DNA libraries were analysed by 16S rRNA gene sequencing on the MiSeq (Illumina) platform. Results There were no significant differences in the composition of bacterial communities from both caries‐affected and caries‐free children with asthma. The “caries‐enriched” genus was Veillonella (Veillonellaceae, Selenomonadales, Negativicutes). Relative abundance of Neisseria was significantly higher in caries‐free children with asthma (p < 0.05). Conclusions The most significant difference in compared bacterial communities was a higher relative abundance of Veillonella in caries‐affected plaques that suggests its involvement in pathogenesis of caries. Potential respiratory pathogens are present in oral cavity of both caries‐affected and caries‐free asthmatic children.

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“…More evidence is now emerging regarding the prevalence and epidemiology of Scardovia wiggsiae, particularly among children with early childhood caries (ECC) [7,8]. These studies clearly describe the potential for caries development and pathology among children and teenagers both in the presence and absence of other clearly defined cariogenic organisms, such as Streptococcus mutans [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Oral Site Specific Sampling Reveals Differential Location for Scardovia wiggsiae

Carr

Alexander

Nguyen

et al. 2020

MRJI

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Introduction: The newly discovered cariogenic pathogen Scardovia wiggsiae has been the subject of intense scientific interest due to the role it may play in the development or progression of caries and oral disease. The primary objective of this study was to perform DNA microbial screening from five specific oral sites, including the gingival crevice between the upper central incisors, biofilm of the upper first molar and lingual incisor, as well as the dorsum of the tongue – for comparison with unstimulated saliva. These data may provide significant insights into site-specific oral locations that harbor S. wiggsiae. Methods: More than one hundred previously collected clinical samples (n=105) were identified for inclusion in this study. DNA isolates were screened using a NanoDrop spectrophotometer to determine overall DNA quantity and quality. Samples with sufficient quality and quantity were screened for the presence of S. wiggsiae using validated PCR primers. Results: More than one hundred patient samples (n=105) were identified, which were comprised of mostly female (57%) versus male (43%) and minority (71%) versus White (29%). The average DNA concentrations ranged between 13.74 and 14.69 mg/mL, with A260:A280 ratios ranging between 1.62 – 1.70. Results of molecular screening using S. wiggsiae specific primers demonstrated only a small percentage of pooled samples (7.6%) harbored this DNA, which was highly concentrated among the samples from tooth surfaces (Upper First Molar, Lingual Incisor) and saliva compared with the gingival crevice and dorsum of the tongue. Discussion: These data provide novel information regarding specific oral locations, including tooth surfaces that harbor S. wiggsiae. In addition, these sites also provide new information regarding oral sites that do not appear to harbor this organism, including the gingival crevice and dorsum of the tongue. This information may be particularly useful to oral health researchers as they strive to limit and reduce the cariogenic microbiome among high-risk populations.

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Functional profiles of coronal and dentin caries in children

Cited by 36 publications

References 44 publications

Quantitative Analysis of Salivary Oral Bacteria Associated with Severe Early Childhood Caries and Construction of Caries Assessment Model

Quantitative Analysis of Salivary Oral Bacteria Associated with Severe Early Childhood Caries and Construction of Caries Assessment Model

Oral microbiomes in children with asthma and dental caries

Oral Site Specific Sampling Reveals Differential Location for Scardovia wiggsiae

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