Comparison of intermittent-bolus contrast imaging with conventional power Doppler sonography: quantification of tumour perfusion in small animals

Krix, Martin; Kießling, Fabian; Vosseler, Silvia; Kiessling, Isabel; Le-Huu, Martin; Fusenig, Norbert E.; Delorme, Stefan

doi:10.1016/s0301-5629(03)00060-7

Cited by 52 publications

(38 citation statements)

References 44 publications

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“…The diagnostic probe permitted monitoring of the tumor agent concentration during exposure, which enabled the assessment of short-term effects of the treatment on blood flow. Krix et al [13] examined the use of contrast bolus kinetics in murine tumors and found that measures of the slope of the initial bolus rise and peak were correlated to measures of destructionreperfusion kinetics. Here we used the use of the first part of the bolus for ROI (central and peripheral regions) flow analysis provide this analysis, and the remainder is used for therapy.…”

Section: Methodsmentioning

confidence: 98%

Antivascular effects of pulsed low intensity ultrasound and microbubbles in mouse tumors

Goertz

Karshafian

Hynynen

2008

2008 IEEE Ultrasonics Symposium

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In this study we investigate the feasibility of inducing transient and sustained blood flow changes in murine tumors with pulsed low intensity ultrasound. Subcutaneous xenograft melanoma tumors were situated in the hind limbs of mice and exposed to 1 MHz (740 kPa) pulsed ultrasound following a bolus injection of Definity™ microbubbles. Flow within the tumors was monitored using a 9 MHz array probe in contrast imaging mode following the bolus injection of agent during treatment, and at 2 and 24 hours post treatment. The contrast enhancement was quantified in ROIs encompassing either the periphery or center of the tumors. The results demonstrate the feasibility of producing transient reductions of flow in tumors lasting for less than 15 minutes and, with repeated exposures, the ability to induce sustained reductions in contrast enhancement in lasting for at least 24 hours. Both short and long term flow modifications have implications for the guidance and enhancement of microbubble potentiated local drug enhancement.

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Section: Methodsmentioning

confidence: 98%

Antivascular effects of pulsed low intensity ultrasound and microbubbles in mouse tumors

Goertz

Karshafian

Hynynen

2008

2008 IEEE Ultrasonics Symposium

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“…In laboratory animals, IV injected US contrast agents have previously been used to visualize blood flow in tumor microvessels (Chomas et al 2003;Fleischer 2000;Forsberg et al 2004;Kamotani et al 2003;Krix et al 2003). Also, differences in perfusion between regions in a tumor have been demonstrated (Chomas et al 2003) and have been quantified using image-gating methods (Kamotani et al 2003).…”

Section: Introductionmentioning

confidence: 98%

The antivascular action of physiotherapy ultrasound on murine tumors

Wood

Ansaloni

Ziemer

et al. 2005

Ultrasound in Medicine & Biology

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This study was aimed at determining if physiotherapy ultrasound (US) affected the fragile and leaky angiogenic blood vessels in a tumor. In 22 C3HV/HeN mice, a subcutaneous melanoma (K1735(22)) was insonated (1, 2 or 3 min) with continuous 1-MHz low-intensity (spatial-average temporal-average = 2.28 W cm(-2)), physiotherapy US. Contrast-enhanced (0.1 mL Optison) power Doppler US observations were made and histogram analyses of the images were performed. Before insonation, all but 7% of the tumor was perfused. The avascular area in tumors receiving 3-min treatment increased to 82% (p < 0.001). A linear regression analysis showed that each min of insonation led to a 25% reduction in tumor vascularity; the antivascular activity persisted for 24 h. Histology demonstrated disruption of vascular walls and tumor cell death in areas of vascular congestion and thrombosis. Physiotherapy US particularly targeted the vascular structures, and the effects on tumor cells appeared to be secondary to the resultant ischemia.

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“…Analysis of replenishment kinetics has been now implemented in US analysis tools. We could show that the analysis of replenishment kinetics is even possible after a single bolus injection of microbubbles instead of the otherwise used continuous infusion [10]. Furthermore, a new multi-vessel model to mathematically analyse the replenishment curves provides a more valid and detailed quantification [11] of the arterial and portal venous perfusion of the liver using low-MI imaging [12].…”

Section: Methods For Microvascular Imagingmentioning

confidence: 94%

Quantification of enhancement in contrast ultrasound: a tool for monitoring of therapies in liver metastases

Krix

2005

Eur Radiol Suppl

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Ultrasound techniques are presented using the contrast agent SonoVue (Bracco, Italy) for microvascular imaging of liver metastases and for the detection of treatment-related changes in tumour perfusion. Technical requirements and examination protocols for contrast-enhanced ultrasound (CEUS) in the detection of microvascularity are described. Procedures for the quantitative analysis of perfusion in follow-up examinations are explained. Compared to contrast-enhanced computed tomography, low-MI (mechanical index) CEUS with SonoVue better reflected the arterial perfusion of liver metastases. Changes of tumour perfusion after stereotactic radiotherapy or chemotherapy of liver metastases could be sensitively detected using CEUS. In addition, possible changes of the liver perfusion after therapy could be assessed. Replenishment kinetics of the contrast agent allowed for a quantitative analysis of the arterial and portal venous perfusion in the tumour and the liver tissue. CEUS is a promising method to non-invasively monitor systemic or local-ablative therapies of liver metastases by sensitively detecting changes of tumour perfusion.

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Comparison of intermittent-bolus contrast imaging with conventional power Doppler sonography: quantification of tumour perfusion in small animals

Cited by 52 publications

References 44 publications

Antivascular effects of pulsed low intensity ultrasound and microbubbles in mouse tumors

Antivascular effects of pulsed low intensity ultrasound and microbubbles in mouse tumors

The antivascular action of physiotherapy ultrasound on murine tumors

Quantification of enhancement in contrast ultrasound: a tool for monitoring of therapies in liver metastases

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