Comparison of Islet Characterization from Use of Standard Crude Collagenase to GMP-Grade Collagenase Enzyme Blends in Preweaned Porcine Islet Isolations

Corrales, Nicole; Park, Sumin; Lau, Hien; Xu, Ivana; Luong, Colleen; Rodriguez, Samuel; Mönch, Johanna; Alexander, Michael P.; Lakey, Jonathan R. T.

doi:10.1177/0963689720977835

Cited by 2 publications

(2 citation statements)

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“…Several research groups studied islet xenotransplantation using pigs as the source of insulin‐producing cells 8 . There has been considerable research exploring the use of purified enzyme blends in islet isolation that have been specifically developed for islet isolation in an attempt to improve consistency and increase yields and function of isolated islets 9 . Since 2006, a total of 9 groups performed non‐human primate experiments using porcine islets.…”

Section: Introductionmentioning

confidence: 99%

“… 8 There has been considerable research exploring the use of purified enzyme blends in islet isolation that have been specifically developed for islet isolation in an attempt to improve consistency and increase yields and function of isolated islets. 9 Since 2006, a total of 9 groups performed non‐human primate experiments using porcine islets. In 2015, Shin JS et al 10 reported on islets isolated from two pigs transplanted into 4‐ to 6‐kg non‐human primates (NHPs).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of islet isolation result and clinical applicability according to GMP‐grade collagenase enzyme blend in adult porcine islet isolation and culture

Kwak

Park

Shim

et al. 2021

Xenotransplantation

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Background Porcine islet xenotransplantation is a promising treatment for type 1 diabetes as an alternative to human pancreatic islet transplantation and long‐term insulin therapy. Several research groups have explored porcine islets as an alternative to the inconsistent and chronic shortage of pancreases from human organ donors. Studies have confirmed successful transplant of porcine islets into non‐human primate models of diabetes; however, in most cases, they require more than one adult porcine donor to achieve sufficient viable islet mass for sustained function. The importance of GMP‐grade reagents includes the following: specific enzymes utilized in the pancreatic isolation process were identified as a key factor in successful human clinical islet transplantation trials using cadaveric islets. As xenotransplantation clinical research progresses, isolation reagents and digestion enzymes play a key role in the consistency of the product and ultimately the outcome of the islet xenotransplant. In this study, we evaluated several commercially available enzyme blends that have been used for islet isolation. We evaluated their impact on islet isolation yield and subsequent islet function as part of our plan to bring xenotransplantation into clinical xenotransplantation trials. Methods Adult porcine islets were isolated from 16 to 17‐month‐old Yucatan miniature pigs following standard rapid procurement. Pigs weighed on average 48.71 ± 2.85 kg, and the produced pancreases were 39.51 ± 1.80 grams (mean ± SEM). After ductal cannulation, we evaluated both GMP‐grade enzymes (Collagenase AF‐1 GMP grade and Liberase MTF C/T GMP grade) and compared with standard non‐GMP enzyme blend (Collagenase P). Islet quality control assessments including islet yield, islet size (IEQ), membrane integrity (acridine orange/propidium iodide), and functional viability (GSIS) were evaluated in triplicate on day 1 post‐islet isolation culture. Results Islet yield was highest in the group of adult pigs where Collagenase AF‐1 GMP grade was utilized. The mean islet yield was 16 586 ± 1391 IEQ/g vs 8302 ± 986 IEQ/g from pancreases isolated using unpurified crude Collagenase P. The mean islet size was higher in Collagenase AF‐1 GMP grade with neutral protease than in Collagenase P and Liberase MTF C/T GMP grade. We observed no significant difference between the experimental groups, but in vitro islet function after overnight tissue culture was significantly higher in Collagenase AF‐1 GMP grade with neutral protease and Liberase MTF C/T GMP grade than the crude control enzyme group. As expected, the GMP‐grade enzyme has significantly lower endotoxin levels than the crude control enzyme group when measured. Conclusions This study validates the importance of using specifically blended GMP grade for adult pig islet isolation for xenotransplantation trials and the ability to isolate a sufficient number of viable islets from one adult pig to provide a sufficient number for islets for a clinical islet transplantation. GMP‐grade enzymes are highl...

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Section: Introductionmentioning

confidence: 99%