Comparison of ladderane phospholipid and core lipids as indicators for anaerobic ammonium oxidation (anammox) in marine sediments

Jaeschke, Andrea; Rooks, Christine; Trimmer, Mark; Nicholls, Joanna C.; Hopmans, Ellen C.; Schouten, Stefan; Damsté, Jaap S. Sinninghe

doi:10.1016/j.gca.2009.01.013

Cited by 50 publications

(65 citation statements)

References 35 publications

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“…For more details on this HPLC/MS-MS method, see Supplementary methods. The C 20 -[3]-monoether ladderane lipid containing a PC headgroup (PC-monoether ladderane) (Supplementary Figure S1) was analyzed by HPLC/electrospray ionization/MS/MS using an SRM method described previously (Jaeschke et al, 2009). …”

Section: Extraction and Analysis Of Iplsmentioning

confidence: 99%

“…IPLs consist of the core membrane lipids still covalently bound to polar head groups that are relatively labile (that is, unstable outside of the intact cell; White et al, 1979), and thus more accurately represent lipids synthesized by living or recently living cells. We developed a novel highperformance liquid chromatography/mass spectrometry/mass spectrometry (HPLC/MS/MS) method to target AOA-specific IPLs, as well as using a previously published method targeting a C 20 -[3]-monoether ladderane lipid containing a phosphatidylcholine (PC) head group (henceforth referred to as 'PC-monoether ladderane') specific for anammox bacteria (Jaeschke et al, 2009). This study was complemented with the quantification of 16S rDNA and functional genes (AOA amoA and hzo genes of anammox bacteria, which encode for the enzyme hydrazine oxidoreductase converting hydrazine to N 2 ) and their expression (that is, RNA abundance) as an indication of the activity of AOA and anammox bacteria.…”

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confidence: 99%

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Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone

et al. 2011

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Ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing (anammox) bacteria have emerged as significant factors in the marine nitrogen cycle and are responsible for the oxidation of ammonium to nitrite and dinitrogen gas, respectively. Potential for an interaction between these groups exists; however, their distributions are rarely determined in tandem. Here we have examined the vertical distribution of AOA and anammox bacteria through the Arabian Sea oxygen minimum zone (OMZ), one of the most intense and vertically exaggerated OMZs in the global ocean, using a unique combination of intact polar lipid (IPL) and gene-based analyses, at both DNA and RNA levels. To screen for AOA-specific IPLs, we developed a high-performance liquid chromatography/mass spectrometry/mass spectrometry method targeting hexose-phosphohexose (HPH) crenarchaeol, a common IPL of cultivated AOA. HPH-crenarchaeol showed highest abundances in the upper OMZ transition zone at oxygen concentrations of ca. 5 lM, coincident with peaks in both thaumarchaeotal 16S rDNA and amoA gene abundances and gene expression. In contrast, concentrations of anammox-specific IPLs peaked within the core of the OMZ at 600 m, where oxygen reached the lowest concentrations, and coincided with peak anammox 16S rDNA and the hydrazine oxidoreductase (hzo) gene abundances and their expression. Taken together, the data reveal a unique depth distribution of abundant AOA and anammox bacteria and the segregation of their respective niches by 4400 m, suggesting no direct coupling of their metabolisms at the time and site of sampling in the Arabian Sea OMZ.

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Section: Extraction and Analysis Of Iplsmentioning

confidence: 99%

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confidence: 99%

Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone

et al. 2011

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“…For example, in the permanently cold sediment of Young Sound, Greenland, with a temperature of <1°C year round, the temperature optimum for anammox was 12°C , and in the Skagerrak, with annual temperatures between 4°C and 6°C, the optimum temperature was 15°C . Recently, anammox activity, ladderane lipids, and 16S rRNA genes were observed at hydrothermal vents and in hot springs which have expanded the temperature ranges significantly (Jaeschke et al 2009b;Byrne et al 2009). The wide range in growth temperature of anammox bacteria may promote their existence in wetlands.…”

Section: Temperaturementioning

confidence: 99%

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Zhu

Jetten

Kuschk

et al. 2010

Appl Microbiol Biotechnol

171

108

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Anaerobic ammonium oxidation (anammox) and anaerobic methane oxidation (ANME coupled to denitrification) with nitrite as electron acceptor are two of the most recent discoveries in the microbial nitrogen cycle. Currently the anammox process has been relatively well investigated in a number of natural and man-made ecosystems, while ANME coupled to denitrification has only been observed in a limited number of freshwater ecosystems. The ubiquitous presence of anammox bacteria in marine ecosystems has changed our knowledge of the global nitrogen cycle. Up to 50% of N 2 production in marine sediments and oxygendepleted zones may be attributed to anammox bacteria. However, there are only few indications of anammox in natural and constructed freshwater wetlands. In this paper, the potential role of anammox and denitrifying methanotrophic bacteria in natural and artificial wetlands is discussed in relation to global warming. The focus of the review is to explore and analyze if suitable environmental conditions exist for anammox and denitrifying methanotrophic bacteria in nitrogen-rich freshwater wetlands.

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“…C 16 -[3]-ladderane fatty acid (III) was approximately 1.5 times lower in abundance than component II. The method was then used to analyze sediments derived from various locations where C 18 and C 20 anammox lipids have previously been detected, i.e., off northwest Africa (08°54.0 0 N, 14°56.1W; 1-2 cmbsf), the Arabian Sea (22°32.9 0 N, 64°02.8 0 E; 193 cmbsf), and the Irish Sea (53°53.0 0 N, 5°35.6 0 W; 1-2 cmbsf) (Jaeschke et al, 2007(Jaeschke et al, , 2009. As shown in the partial SRM traces in Fig.…”

Section: Environmental Occurrence Of Short-chain Ladderane Fatty Acidsmentioning

confidence: 99%

Short chain ladderanes: Oxic biodegradation products of anammox lipids

Rush

Jaeschke

Hopmans

et al. 2011

Geochimica et Cosmochimica Acta

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Anammox, the microbial anaerobic oxidation of ammonium by nitrite to produce dinitrogen gas, has been recognized as a key process in both the marine and freshwater nitrogen cycles, and found to be a major sink for fixed inorganic nitrogen in the oceans. Ladderane lipids are unique anammox bacterial membrane lipids that have been used as biomarkers for anammox bacteria in recent and past environmental settings. However, the fate of ladderane lipids during diagenesis is as of yet unknown. In this study, we performed oxic degradation experiments (at 20-100°C) with anammox bacterial biomass to simulate early diagenetic processes occurring in the water column and at the sediment-water interface. Abundances of C 18 and C 20 ladderane lipids decreased with increasing temperatures, testifying to their labile nature. The most abundant products formed were ladderane lipids with a shorter alkyl side chain (C 14 and C 16 ladderane fatty acids), which was unambiguously established using two-dimensional NMR techniques on an isolated C 14 -[3]-ladderane fatty acid. The most pronounced production of these short-chain lipids was at 40°C, suggesting that degradation of ladderane lipids was microbially mediated, likely through a b-oxidation pathway. An HPLC-MS/MS method was developed for the detection of these ladderane alteration products in environmental samples and positively tested on various sediments. This showed that the ladderanes formed during degradation experiments also naturally occur in the marine environment. Thus, short-chain ladderane lipids may complement the original longer-chain ladderane lipids as suitable biomarkers for the detection of anammox processes in past depositional environments.

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Comparison of ladderane phospholipid and core lipids as indicators for anaerobic ammonium oxidation (anammox) in marine sediments

Cited by 50 publications

References 35 publications

Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone

Niche segregation of ammonia-oxidizing archaea and anammox bacteria in the Arabian Sea oxygen minimum zone

Potential roles of anaerobic ammonium and methane oxidation in the nitrogen cycle of wetland ecosystems

Short chain ladderanes: Oxic biodegradation products of anammox lipids

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