Comparison of lignin deposition in three ectopic lignification mutants

Abstract

Summary• The Arabidopsis thaliana mutants de-etiolated3 ( det3 ), pom-pom1 ( pom1 ) and ectopic lignification1 ( eli1 ) all deposit lignins in cells where these polymers would not normally be found. Comparison of these mutants provides an opportunity to determine if the shared mutant phenotype arose by perturbing a common regulatory mechanism in each of the mutants.• The mutants were compared using a combination of genetics, histochemistry, chemical profiling, transcript profiling using both Northern blots and microarrays, and bioinformatics.• The subset of cells that ectopically lignified was shared between all three mutants, but clear differences in cell wall chemistry were evident between the mutants. Northern blot analysis of lignin biosynthetic genes over diurnal and circadian cycles revealed that transcript abundance of several key genes was clearly altered in all three mutants. Microarray analysis suggests that changes in the expression of specific members of the R2R3-MYB and Dof transcription factor families may contribute to the ectopic lignification phenotypes.• This comparative analysis provides a suite of hypotheses that can be tested to examine the control of lignin biosynthesis.

Chitinase-Like Protein CTL1 Plays a Role in Altering Root System Architecture in Response to Multiple Environmental Conditions

“…Interestingly, reduction of cellulose synthesis by inhibitors or in the eli1-1 (cesa3) mutant background resulted in the accumulation of ectopic lignin deposition (CanoDelgado et al, 2003;Rogers et al, 2005). Thus, we examined the conditional nature of the lignin deposition in roots on the nitrate availability.…”

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

“…The cell death in roots was determined using propidium iodide (PI) treatment with minor modifications from the method of Kwon et al [15]. For the in vivo detection of lignification of roots, fresh roots were incubated in 1% (w/v) phloro-Acta Biologica Hungarica 67, 2016 glucinol-HCl solution for 5 min as described by Rogers et al [25]. The DHE, HPF and PI treated roots were photographed with a fluorescence microscope and associated software (BX61-32 FDIC, Olympus, Japan).…”

Response to copper excess in Arabidopsis thaliana: Impact on the root system architecture, hormone distribution, lignin accumulation and mineral profile

“…For lignin staining, samples were rinsed in distilled water for 5 min and then incubated in a 1% (w/v) phloroglucinoleHCl 6 N solution for 5 min [59].…”