Comparison of Lipase Production byEnterococcus faeciumMTCC 5695 andPediococcus acidilacticiMTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology

Abstract

A medium using fish waste as substrate was designed for production of lipase by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361. Medium components and culture conditions (fish waste protein hydrolysate (FWPH) concentration, fish waste oil (FWO) concentration, pH, temperature, and fermentation time) which affect lipase production were screened using factorial (5 factors ∗ 2 levels) design of which FWPH concentration, FWO concentration, and fermentation time showed significance (P < 0.05). The levels of these factors were optimized further by Box-Behnken design using response surface methodology (RSM). Optimized conditions were found to be 5% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation time for MTCC 5695, and 4% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation for MTCC 11361, which were further validated. Under optimized conditions, MTCC 5695 and MTCC 11361 showed 3.15- (543.63 to 1715 U/mL) and 2.3- (214.74 to 493 U/mL) fold increase in lipase production, respectively, as compared to unoptimized conditions.

Statistical modelling for optimized lyophilization of Lactobacillus acidophilus strains for improved viability and stability using response surface methodology

“…( 2 )–( 8 ). The graphical representation of results in 2D contour plots and 3D surface plots of the regression model indicated the interactions between model terms A and B (Ramakrishnan et al 2012 , 2013 ).…”

Statistical enhancement of lipase extracellular production byBacillus stratosphericusPSP8 in a batch submerged fermentation process

“…But it was lower than the 1Á8-, 2Á4-, 2Á5-, 3Á15-, 5-, 3Á54and 1Á83-fold increase obtained by Staphylococcus arlettae JPBW-1, Burkholderia cepacia, Debaryomyces hansenii, Enterococcus faecium MTCC 5695, Burkholderia sp., Bacillus sp. KS4 and B. cereus, respectively, using RSM optimization (Rathi et al 2002;Chauhan et al 2013;Papagora et al 2013;Ramakrishnan et al 2013;Sharma et al 2014;Vasiee et al 2016).…”

Identification of lipolytic enzymes isolated from bacteria indigenous to Eucalyptus wood species for application in the pulping industry

“…The catalytic triad is comprised of Ser-Asp-His (Glu instead of Asp for some lipases) and typically also a consensus sequence (Gly-x-Ser-x-Gly) is found around the active site serine [4] . These lipolytic enzymes have been isolated from plants, animals, and microorganisms [1] , [5] , however, microbial lipolytic enzymes are reported to be more robust in nature than plant or animal enzymes [6] , [7] . They are also appealing due to their low cost of production and they are simple to manipulate [1] .…”