Statistical enhancement of lipase extracellular production by<i>Bacillus stratosphericus</i>PSP8 in a batch submerged fermentation process

Ismail, Abdallah R.; Younis, Sherif A.; Betiha, Mohamed A.; Azab, Mohamed Salah; Sedky, Nagwa M.

doi:10.1111/jam.14023

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…But it was in contradiction with Kim et al 25 who reported the optimum temperature range of B. glycinifermentans as 15-55˚C. Our results are in contradiction with the studies of Shivaji et al 28 and Ismail et al 29 who reported its optimum growth conditions of lipase producing Bacillus species as 8-37˚C, pH 6-10 and 34.8˚C, and pH 6.98, respectively. B. glycinifermentans-MK840989 cells were induced by olive oil (Figure 2) which agrees with Liaqat et al 19 but contradicts with the findings of Iqbal and Rehman, 9 who observed slow growth of B. subtilis I-4 cells in the presence of olive oil.…”

Section: Lipase Quantification and Selectioncontrasting

confidence: 99%

Thermostable acidic lipase of Bacillus glycinifermentans-MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications

Ali¹,

Shamim²,

Mehboob³

et al. 2020

MOJES

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This study is the first report about isolation, purification and optimization of lipase from Bacillus glycinifermentans. In this study, Bacillus glycinifermentansMK-840989 was isolated from a local petrol pump. The bacterium showed lipolytic zones of 0.19cm, 0.044cm, and 0.28cm on peptone yeast agar, olive oil hydrolysis agar and chromogenic plate agar, respectively. B. glycinifermentans also produced an extracellular lipase (55.1µmol/ml). This bacterium preferred acidic environment (pH 5) for growing optimally at 80˚C when the medium was supplemented with 1% olive oil. The olive oil induced its growth up to 9h. The protein content of the purified lipase was estimated about 75mg/ml as compared to its crude form, i.e. 350mg/ml. The purified lipase was found to be thermostable acidic in nature as its optimum activity was observed at 90˚C (0.08U/ml) and pH 5 (0.02U/ml). Other optimization factors included 1% olive oil (0.065U/ml), 0.1mM maltose (0.023U/ml), 0.1mM Ca (0.025U/ml), 1% yeast extract (16.8U/ml), 1% wheat waste (0.019U/ml), 1% commercial detergent (0.016U/ml) and 1% tween-20 (0.015 U/ml). The purified lipase showed a polypeptide of 26.7kDa on SDS-PAGE. These features such as thermostability, acidic nature, ability to show activity in wheat waste and tolerance to detergents render the lipase of B.glycinifermentans MK-840989 as an attractive choice for biotechnologists to employ it at industrial level. The purified lipase of B.glycinifermentans MK-840989 can be a potential candidate for detergent and oil-remediation industry. It can help to replace conventional synthetic detergent as it is cost-effective and eco-friendly.

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Section: Lipase Quantification and Selectioncontrasting

confidence: 99%

Thermostable acidic lipase of Bacillus glycinifermentans-MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications

Ali¹,

Shamim²,

Mehboob³

et al. 2020

MOJES

View full text Add to dashboard Cite

show abstract

“…Response surface methodology was used to optimize the screened variables and studying their interaction effects for enhanced lipase production by SSF. This method involves performing the statistically designed experiments, estimating the mathematical coefficients, predicting the response and checking the adequacy of the model [26]. Design Expert v9.0.5.1 is used to design the experiments of Central Composite Face-Centered Design.…”

Section: Central Composite Face-centered Designmentioning

confidence: 99%

Sequential optimization and large scale production of lipase using tri-substrate mixture from Aspergillus niger MTCC 872 by solid state fermentation

Mandari

Nema

Devarai

2020

Process Biochemistry

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“…Several bacteria produce lipases that can hydrolyze triglycerides [ 41 ]. Bacteria belonging to various genera are widely reported for the production of lipases [ 42 ]. Among them, the extracellular lipases produced by Bacillus spp.…”

Section: Resultsmentioning

confidence: 99%

Sodium Alginate–Gelatin Nanoformulations for Encapsulation of Bacillus velezensis and Their Use for Biological Control of Pistachio Gummosis

2022

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Biopolymer-based nanocomposites are favorable materials for the encapsulation of biofertilizers and biocontrol agents. In this research, sodium alginate, a widely used natural polymer, was extracted and purified from Macrocystis pyrifera. Its composition was confirmed using 1H NMR and FTIR analyses, and its molecular weight and mannuronic acid/guluronic acid ratio were obtained. Sodium alginate–gelatin microcapsules enriched with carbon nanotubes and SiO2 nanoparticles were prepared to encapsulate Bacillus velezensis, and the biological effects of this formulation on the control of pistachio gummosis and growth parameters were investigated. Microscopy examination showed that the microcapsules had quite globular shapes. XRD confirmed the occurrence of an electrostatic interaction when sodium alginate was blended with gelatin. The survival rate of the encapsulated bacteria was about 107 CFU/mL and was maintained after one year of storage. The aim of this study was to achieve a unique formulation containing beneficial bacteria and nanoparticles for the synergistic control of Phytophthora drechsleri.

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Statistical enhancement of lipase extracellular production byBacillus stratosphericusPSP8 in a batch submerged fermentation process

Abstract: The RSM-CCF statistical optimization is useful for optimizing a large number of variables and studying their interactive effects on extracellular lipase production.

Cited by 10 publications

References 36 publications

Thermostable acidic lipase of Bacillus glycinifermentans-MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications

Thermostable acidic lipase of Bacillus glycinifermentans-MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications

Sequential optimization and large scale production of lipase using tri-substrate mixture from Aspergillus niger MTCC 872 by solid state fermentation

Sodium Alginate–Gelatin Nanoformulations for Encapsulation of Bacillus velezensis and Their Use for Biological Control of Pistachio Gummosis

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