Comparison of lipase production on crambe oil and meal by Fusarium sp. (Gibberella fujikuroi complex)

Abstract

This study provides a comparison of fermentation conditions for the production lipases by Fusarium sp. (Gibberella fujikuroi complex) isolate FCLA-MA-41 in submerged (SmF) and solid-state fermentation (SSF) using agro-industrial residues. To assess SmF, a univariate study of seven carbon sources (crambe, corn, linseed, olive, palm (dendê), soybean oils and chicken fat) and four nitrogen sources (ammonium sulfate, sodium nitrate, urea, yeast extract) was performed. The relationship among the concentrations of carbon and nitrogen sources, Triton X-100 and yeast extract was studied using a Central Composite Design (CCD). To assess SSF, different residues (sugarcane bagasse) and oil-seed meals (castorbean, corn, crambe, soybean) with additional supplements added (minimum salts, seed oils) were examined. Based on the CCD matrix, a medium containing crambe oil (17.5 mL/L), Triton X-100 (5 g/L), ammonium sulfate (5 g/L) and yeast extract (1 g/L) was proposed, resulting in a lipase titer of 3.0 AE 0.25 U/mL. The cost/production relationship was 7.73% less expensive than SmF with corn oil. SSF produced a maximum lipase titer of 5.0 AE 0.25 U/gds on crambe meal moistened with phosphate buffer. The best cost/enzyme activity estimate was for SSF with crambe meal as substrate in only distilled water (87.27% less expensive than SmF).

Chicken fat and inorganic nitrogen source for lipase production by Fusarium sp. (Gibberella fujikuroi complex)

“…(GFC) FCLA-MA41 strain in SmF with culture medium containing crambe oil (17.5 mL/L), Triton X-100 (5 g/L), ammonium sulfate (5 g/L) and yeast extract (1 g/L) was proposed, resulting in a lipase titer of 3.0 ± 0.25 U/mL (Oliveira et al, 2013). Although providethis provides lower activity than that obtained with chicken oil, the cost per unit of enzyme activity was also lower ($US 183.00 and $US 518.00/million Units of lipase with crambe oil and chicken fat, respectively).…”

“…(Gibberella fujikuroi complex) FCLA-MA41 was isolated from decaying plant matter in the Atlantic Forest in the state of São Paulo (Brazil) and identified as described by Oliveira et al (2013). The fungal isolate was maintained on MEA medium (malt extract agar) and stored at 4°C.…”

“…Recently a strain of Fusarium sp. (Gibberella fujikuroi complex) FCLA-MA41 (Oliveira et al, 2013) was isolated from decaying plant matter in the Atlantic Forest of the state of São Paulo in Brazil. Cultivation of the strain in Submerged Fermentation, a medium containing crambe oil (17.5 mL/L), Triton X-100 (5 g/L), ammonium sulfate (5 g/L) and yeast extract (1 g/L) was proposed, resulting in a lipase titer of 3.0 ± 0.25 U/mL.…”

Microbial biosynthesis of enzymes for food applications

“…Lipases also contribute to the development of a distinctive flavor during the ripening stage of food production, which acts on butterfat to produce the characteristic flavors of several cheese types (Binod et al, 2013). (Oliveira et al, 2013), and P. chrysogenum, Trichoderma harzianum, and A. flavus (Toscano et al, 2013). (Oliveira et al, 2013), and P. chrysogenum, Trichoderma harzianum, and A. flavus (Toscano et al, 2013).…”

“…Oil seed cake (Venkatesagowda et al, 2014), rice bran, wheat bran, canola seed oil cake, sunflower hulls, peanut shells (Amin and Bhatti, 2014), pumpkin seed flour (Zubiolo et al, 2014), jatropha seed cake (Veerabhadrappa et al, 2014), crambe oil, corn oil, linseed oil, olive oil, palm oil, soybean oil, chicken fat (Oliveira et al, 2013), mustard oil cake (Imandi et al, 2013), sesame oil cake, groundnut oil cake, coconut oil cake (Rajendran and Thangavelu, 2013), and fish waste meal (Esakkiraj et al, 2012) have been reported as raw materials and carbon sources for microbial lipase production in the past few years.…”