Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice

Zhao, Zijiao; Chen, Lu; Cao, Maosheng; Chen, Tong; Huang, Yiqiu; Wang, Nan; Zhang, Boqi; Li, Fangxia; Chen, Kaimin; Yuan, Chenfeng; Li, Chunjin; Zhou, Xu

doi:10.3390/ani12030399

Cited by 4 publications

(3 citation statements)

References 70 publications

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“…In this study, we found that Hand2os1 was highly expressed in the luteal cells of mice during embryo implantation and delivery. This is consistent with the results of a previous study, in which some lncRNAs were found to play a role in embryo implantation and labor through immune responses [25]. These results suggest that Hand2os1 may be involved in embryo attachment and delivery mechanisms.…”

Section: Discussionsupporting

confidence: 93%

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

Jia

Liu

Gong

et al. 2022

Veterinary Sciences

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The corpus luteum plays a key role in pregnancy maintenance and estrous cycle regulation by secreting progesterone. Hand2os1 is an lncRNA located upstream of Hand2, with which a bidirectional promoter is shared and is involved in the regulation of cardiac development and embryo implantation in mice. The aim of this study was to investigate the expression and regulation of Hand2os1 in the ovaries. Here, we used RNAscope to detect differential expression of Hand2os1 in the ovaries of cycling and pregnant mice. Hand2os1 was specifically detected in luteal cells during the proestrus and estrus phases, showing its highest expression in the corpus luteum at estrus. Additionally, Hand2os1 was strongly expressed in the corpus luteum on day 4 of pregnancy, but the positive signal progressively disappeared after day 8, was detected again on day 18, and gradually decreased after delivery. Hand2os1 significantly promoted the synthesis of progesterone and the expression of StAR and Cyp11a1. The decreased progesterone levels caused by Hand2os1 interference were rescued by the overexpression of StAR. Our findings suggest that Hand2os1 may regulate the secretion of progesterone in the mouse corpus luteum by affecting the key rate-limiting enzyme StAR, which may have an impact on the maintenance of pregnancy.

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Section: Discussionsupporting

confidence: 93%

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

Jia

Liu

Gong

et al. 2022

Veterinary Sciences

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“…Moreover, m6A modification is widely occurred in many other RNA species [43]. Notably, some long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) also play important roles in uterus during early pregnancy [44, 45]. It is highly possible that these noncoding RNAs are also subject to epigenetic m6A regulation.…”

Section: Discussionmentioning

confidence: 99%

METTL3 promotes proliferation of goat endometrial epithelial cells by regulating CTGF in an m6A-dependent manner

Sun

Zhang

et al. 2023

Biology of Reproduction

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N6-methyladenosine (m6A), an epigenetic modification on RNAs, plays an important role in many physiological and pathological processes. However, the involvement of m6A in goat uterus during early pregnancy remains largely unknown. In this study, we found that the total m6A level was increasing in goat uterus as early pregnancy progressed. Methyltransferase-like 3 (METTL3) is a core catalytic subunit of the m6A methyltransferase. We thus determined the expression and regulation of METTL3 in goat uterus. METTL3 was highly expressed in the luminal and glandular epithelia from day 16 (D16) to D25 of pregnancy. And it could be up-regulated by estrogen and progesterone in goat uterus and primary endometrial epithelial cells (EECs). In EECs, knockdown or overexpression of METTL3 resulted in a significant decrease or increase of cell proliferation, respectively. METTL3 knockdown reduced the m6A level of not only total RNA but also connective tissue growth factor (CTGF) mRNA. Luciferase assay suggested that METTL3 might target the potential m6A sites in the 3’untranslated region (3’UTR) of CTGF mRNA. Moreover, METTL3 positively regulated CTGF expression, and CTGF knockdown significantly counteracted the promoting effect of METTL3 overexpression on EEC proliferation. Collectively, METTL3 is dynamically expressed in goat uterus and can affect EEC proliferation by regulating CTGF in an m6A-dependent manner. Our results will lay a foundation for further studying the crucial mechanism of METTL3-mediated m6A modification in goat uterus during early pregnancy.

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“…An increasing number of studies have shown that lncRNAs play an influential role in the development of a variety of tissues. For instance: heart ( Klattenhoff et al, 2013 ), liver ( Bu et al, 2020 ), testis ( Mishra et al, 2021 ), kidney ( Sun et al, 2020 ), Mammary gland ( Xu et al, 2018 ), brain ( Ang et al, 2020 ), uterus ( Zhao et al, 2022 ), and skin ( Zhao et al, 2021 ). There also be several lncRNAs related to muscle development and fat deposition have been reported, such as lncRNAs that related with muscle development including lnc403 ( Zhang et al, 2020 ), lnc-H19 ( Xu et al, 2017 ) and lncMD ( Sun et al, 2016 ), lncYYW ( Yue et al, 2017 ), and lncRNAs with fat deposition including lnc-BADLNCR1 ( Cai et al, 2020 ), lncCCPG1 ( Kang et al, 2020 ), and lncRNA ADNCR ( Li et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Long-stranded non-coding RNAs temporal-specific expression profiles reveal longissimus dorsi muscle development and intramuscular fat deposition in Tianzhu white yak

He,

Wang,

et al. 2023

Journal of Animal Science

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The process of muscle development and intramuscular fat (IMF) deposition is quite complex and controlled by both mRNAs and ncRNAs. Long-stranded non-coding RNAs (LncRNAs) are involved in various biological processes in mammals while also playing a critical role in muscle development and fat deposition. In the present study, RNA-Seq was used to comprehensively study the expression of lncRNAs and mRNAs during muscle development and intramuscular fat deposition in postnatal Tianzhu white yaks at three stages, including 6 months of age (calve, n=6), 30 months of age (young cattle, n=6) and 54 months of age (adult cattle, n=6). The results indicated that a total of 2101 lncRNAs and 20855 mRNAs were screened across the three stages, of which the numbers of differential expression (DE) lncRNAs and DE mRNAs were 289 and 1339, respectively, and DE lncRNAs were divided into eight different expression patterns based on expression trends. GO and KEGG enrichment analysis revealed that some DE mRNAs overlapped with target genes of lncRNAs, such as NEDD4L, SCN3B, AGT, HDAC4, DES, MYH14, KLF15 (muscle development), ACACB, PCK2, LIPE, PIK3R1, PNPLA2, MGLL (intramuscular fat deposition). These DE mRNAs were significantly enriched in critical muscle development and IMF deposition related pathways and GO terms, such as AMPK signaling pathway, PI3K-Akt signaling pathway, PPAR signaling pathway, etc. In addition, lncRNA-mRNA co-expression network analysis revealed that six lncRNAs (MSTRG.20152.2, MSTRG.20152.3, XR_001351700.1, MSTRG.8190.1, MSTRG.4827.1 and MSTRG.11486.1) may play a major role in Tianzhu white yak muscle development and lipidosis deposition. Therefore, this study enriches the database of yak lncRNAs and could help to further explore the functions and roles of lncRNAs in different stages of muscle development and intramuscular fat deposition in the Tianzhu white yak.

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Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice

Cited by 4 publications

References 70 publications

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

METTL3 promotes proliferation of goat endometrial epithelial cells by regulating CTGF in an m6A-dependent manner

Long-stranded non-coding RNAs temporal-specific expression profiles reveal longissimus dorsi muscle development and intramuscular fat deposition in Tianzhu white yak

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