Comparison of localized proton NMR signals of skeletal muscle and fat tissue <i>in vivo</i>: Two lipid compartments in muscle tissue

Schick, Fritz; Eismann, Beate; Jung, Wulf‐Ingo; Bongers, H.; Bunse, Michael; Lutz, O.

doi:10.1002/mrm.1910290203

Cited by 333 publications

(326 citation statements)

References 23 publications

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“…While some metabolites show residual dipolar coupling (3,4), lipids experience a shift of the resonances due to bulk magnetic susceptibility effects (2). These findings also helped to further understand the observations made by Schick et al (1).…”

Section: Introductionsupporting

confidence: 58%

“…An initial observation of two compartments of triglycerides or fatty acids in human skeletal muscle with a resonance frequency shift of 0.2 ppm was published by Schick et al (1) in 1993. In order to identify the chemical nature of the signals, spectra of different tissuesskeletal muscle, subcutaneous fat and yellow bone marrow-were recorded using identical sequence timing.…”

Section: Introductionmentioning

confidence: 99%

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Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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1 H-MR spectroscopy (MRS) of intramyocellular lipids (IMCL) became particularly important when it was recognized that IMCL levels are related to insulin sensitivity. While this relation is rather complex and depends on the training status of the subjects, various other influences such as exercise and diet also influence IMCL concentrations. This may open insight into many metabolic interactions; however, it also requires careful planning of studies in order to control all these confounding influences. This review summarizes various historical, methodological, and practical aspects of 1 H-MR spectroscopy (MRS) of muscular lipids. That includes a differentiation of bulk magnetic susceptibility effects and residual dipolar coupling that can both be observed in MRS of skeletal muscle, yet affecting different metabolites in a specific way. Fitting of the intra-(IMCL) and extramyocellular (EMCL) signals with complex line shapes and the transformation into absolute concentrations is discussed. Since the determination of IMCL in muscle groups with oblique fiber orientation or in obese subjects is still difficult, potential improvement with high-resolution spectroscopic imaging or at higher field strength is considered. Fat selective imaging is presented as a possible alternative to MRS and the potential of multinuclear MRS is discussed. 1 H-MRS of muscle lipids allows non-invasive and repeated studies of muscle metabolism that lead to highly relevant findings in clinics and patho-physiology.

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Section: Introductionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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“…One of the major metabolites in in vivo proton MR spectroscopy of muscles is lipids, which are major substrates for energy production both at rest and during muscle contraction (28) . A previous study found that large variations in low‐molecular‐weight species in ex vivo synovial fluid samples were found between individuals and that there was no measurable correlation between disease state and the levels of any low‐molecular‐weight components (22) .…”

Section: Discussionmentioning

confidence: 99%

In vivo MR spectroscopy using 3 Tesla to investigate the metabolic profiles of joint fluids in different types of knee diseases

Jin

Woo

Jahng

2016

J Applied Clin Med Phys

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In vivo proton (H1) magnetic resonance spectroscopy (MRS) has not yet been systematically used to study joint fluids in human knees. The objective of this study, therefore, was to assess the ability of proton MRS to identify the apparent heterogeneous characteristics of metabolic spectra in the joint fluid regions in human knees using a high‐field MRI system. Eighty‐four patients with effusion lesions who were referred for routine knee MR imaging underwent proton MRS with point‐resolved, single‐voxel MR spectroscopy using a clinical 3.0 Tesla MRI system. Thirty‐eight patients were confirmed to have the following: degenerative osteoarthritis, 21 patients (Group 1); traumatic diseases, 12 patients (Group 2); infectious diseases, 4 patients and an inflammatory disease, 1 patient (Group 3). Spectroscopy data were analyzed using the public jMRUI freeware software to obtain lipid metabolites. Nonparametric statistical comparisons were performed to investigate any differences in metabolites among the three disease groups. The major metabolites were vinylic CH=CH lipids around 5.1−5.5 ppm, CH2 lipids around 1.1−1.5 ppm, and CH3 lipids around 0.7−1.0 ppm. Each patient had either a CH=CH lipid peak, CH2 and CH3 lipid peaks, or all three peaks. There were no significant differences among the three groups for the CH3 (normalp=0.9019), CH2 (normalp=0.6406), and CH=CH lipids (p=0.5467) and water (p=0.2853); none of the metabolites could differentiate between any of the three types of diseases. The CH2 lipids in the 38 patients who had confirmed fluid characteristics were significantly correlated with CH3 lipids (italicrho=0.835, p<0.0001). The ratio of CH3 to CH2 was highest in the degenerative disease. In both the degenerative and traumatic diseases, metabolite peaks of the vinylic CH=CH lipids around 5.1−5.5 ppm and of the sum of the CH2 and CH3 lipids around 0.7−1.5 ppm were observed, but in the infectious disease, only a metabolite peak of the sum of the CH2 and CH3 lipids was detected. Although none of the metabolites could statistically significantly differentiate between the three types of diseases, the different lipid metabolite peaks and their ratios in the three disease groups may give us a hint at the different mechanisms of joint fluids in the infectious, degenerative, and traumatic diseases.PACS number(s): 87.61.Ff, 33.25.+k, 87.14.Cc

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“…Knowledge about this physiological role has mainly been acquired from biopsies, so only a few data points have been obtained, due to the invasive nature of this examination. 1 H-MR spectroscopy is now able to determine IMCL in human muscle non-invasively [7][8][9][10][11]14 and repeatedly. Using this technique, it has been found that IMCL levels are extremely variable in response to physical exercise 8,11,15,16 and diet.…”

Section: Susceptibility Effects: Separation Of Imcl and Emclmentioning

confidence: 99%

“…Figure 2 shows how geometric ordering on a macroscopic scale leads to a separation of signals from intra-(IMCL) and extra-myocellular (EMCL) lipids in 1 H-MR spectra. Schick et al 10 observed two lipid signals in 1 H-MR spectra of muscle tissue with a difference of their resonance frequencies of about 0.2 ppm. The authors concluded that the signals might stem from two compartments and made a tentative assignment of one compartment to fat cells within muscle tissue, while the other compartment was hypothesized to be located within muscle cells.…”

Section: Introduction/type Of Order In Musclesmentioning

confidence: 99%

Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle

Boesch

Kreis

2001

NMR in Biomedicine

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Skeletal muscle is a biological structure with a high degree of organization at different spatial levels. This order influences magnetic resonance (MR) in vivo-in particular 1 H-spectra-by a series of effects that have very distinct physical sources and biomedical applications: (a) bulk fat (extramyocellular lipids, EMCL) along fasciae forms macroscopic plates, changing the susceptibility within these structures compared to the spherical droplets that contain intra-myocellular lipids (IMCL); this effect leads to a separation of the signals from EMCL and IMCL; (b) dipolar coupling effects due to anisotropic motional averaging have been shown for 1 H-resonances of creatine, taurine, and lactate; (c) aromatic protons of carnosine show orientation-dependent effects that can be explained by dipolar coupling, chemical shift anisotropy or by relaxation anisotropy; (d) limited rotational freedom and/or compartmentation may explain differences of 1 H-MR-visibility of the creatine/phosphocreatine resonances; (e) lactate 1 H-MR resonances are reported to reveal information on tissue compartmentation; (f) transverse relaxation of water and metabolites show multiple components, indicative of intra-, extracellular and/or macromolecular-bound pools, and in addition dipolar or J-coupling lead to a modulation of the signal decay, hindering straightforward interpretation; (g) diffusion weighted 31 P-MRS has shown restricted diffusion of phosphocreatine; (h) magnetization transfer (MT) indicates that there is a motionally restricted proton pool in spin-exchange with free creatine; reduced availability or restricted motion of creatine is particularly important for an estimation of ADP from 31 P-MR spectra, and in addition MT effects may alter the signal intensity of creatine 1 H-resonances following water-suppression pulses; (i) transcytolemmal water-exchange can be studied in 1 H-MRS by contrast-agents applied to the extracellular space; (k) transport of glucose across the cell membrane has been studied in diabetes patients using a combination of 13 C-and 31 P-MRS; and (l) residual quadrupolar interaction in 23 Na MR spectra from human skeletal muscle suggest that sodium ions are bound to ordered muscular structures.

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Comparison of localized proton NMR signals of skeletal muscle and fat tissue in vivo: Two lipid compartments in muscle tissue

Cited by 333 publications

References 23 publications

Role of proton MR for the study of muscle lipid metabolism

Role of proton MR for the study of muscle lipid metabolism

In vivo MR spectroscopy using 3 Tesla to investigate the metabolic profiles of joint fluids in different types of knee diseases

Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle

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