Comparison of LST + MUG broth technique and conventional method for the enumeration of Escherichia coli in foods

Doğan, Hilal B.; Çakır, İbrahim; Başpınar, Ensar; Halkman, A. Kadir

doi:10.1046/j.1472-765x.2002.01079.x

Cited by 4 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Measurement of the E. coli populations is the best indicator of the fecal contamination of both water (Edberg et al, 2000) and foods (Dogan et al, 2002). Since total commensal E. coli is also an accurate indicator of E. coli O157:H7 environmental persistence (Ogden et al, 2001), we measured the total E. coli population.…”

Section: Methodsmentioning

confidence: 99%

Bedding and Seasonal Effects on Chemical and Bacterial Properties of Feedlot Cattle Manure

et al. 2003

View full text Add to dashboard Cite

Nutrients, soluble salts, and pathogenic bacteria in feedlot-pen manure have the potential to cause pollution of the environment. A three-year study (1998-2000) was conducted at a beef cattle (Bos taurus) feedlot in southern Alberta, Canada to determine the effect of bedding material [barley (Hordeum vulgare L.) straw versus wood chips] and season on the chemical and bacterial properties of pen-floor manure. Manure was sampled for chemical content (N, P, soluble salts, electrical conductivity, and pH) and populations of four groups of bacteria (Escherichia coli, total coliforms, and total aerobic heterotrophs at 27 and 39 degrees C). More chemical parameters of manure were significantly (P < or = 0.05) affected by season (SO4, Na, Mg, K, Ca, sodium adsorption ratio [SAR], total C, NO3-N, NH4-N, total P, and available P) than by bedding (K, pH, total C, C to N ratio, NH4-N, and available P). Bedding had no significant (P > 0.05) effect on the four bacterial groups whereas season affected all four groups. Numbers of E. coli and total coliforms (TC) were significantly higher by 1.72 to 2.02 log10 units in the summer than the other three seasons, which was consistent with a strong positive correlation of E. coli and TC with air temperature. The low ratio of bedding to manure in the pens was probably the major cause of the lack of significant bedding effects. Bedding material and seasonal timing of cleaning feedlot pens and land application of manure may be a potential tool to manage nutrients, soluble salts, and pathogens in manure.

show abstract

Section: Methodsmentioning

confidence: 99%

Bedding and Seasonal Effects on Chemical and Bacterial Properties of Feedlot Cattle Manure

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Here, 500 food samples which were analysed for E. coli enumeration. This study came with results that fluorogenic method is more reliable and shorter to perform than the standard ISO method [20].…”

Section: Analysis Of Raw Milk and Pasteurized Milk Samples By Develop...mentioning

confidence: 84%

Comparison of Standardized X-gal, ONPG and MUG Assay Methods with IS Methods by Analyzing Milk Samples

Gawai

Prajapati²,

Tagalpallewar³

et al. 2022

CJAST

View full text Add to dashboard Cite

Aims: Food Safety Standards Authority of India (FSSAI) in 2011 has adopted a conventional IS-5887 (Part-I) 1976 and IS-5401 Part-1 (2012) protocol for monitoring of E. coli and coliforms in dairy products respectively. These methods are time consuming and sometimes requires further isolation and confirmation to finalize the true contaminant. The current investigation was carried out to compare these methods with developed chromogenic and fluorogenic assay methods to access their suitability in actual analysis in terms of time saving, reliability and reproducibility. Place and Duration of Study: Department of Dairy Microbiology, SMC College of Dairy Science, Anand Agricultural University, Anand, June 2019 to June 2020. Methods: Ten samples of raw and pasteurized milks were inoculated with formulated selective broth. After incubation for 10 hrs crude enzymes were extracted to detect the presence of coliforms. Similarly 0.1 ml 4-Methylumbelliferyl-β-D-Glucuronide (MUG) solution was added in Formulated selective broth for the detection of E. coli. Enzyme extract procedure was not required after incubation of sample for detection of E. coli. Then after presence of coliforms were detection by the X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) impregnated strips and quantification was done by the chart developed using optical density measurement in o-nitrophenyl-ß-d-galactopyranoside (ONGP) assay. For E. coli detection, blue fluorescence generated was measured under UV light at 350 nm and quantification was done by measuring relative light unit (RLU) generated by Fluorescence spectrophotometer. Same sample was also analyzed by standard IS 5401: part-1 (2012) and IS 5887: Part - I (1976; reaffirmed 2005) procedure and compared with standardized methods for enumeration of coliforms and E. coli respectively. Results: This study made clear that the results of analysis of raw and pasteurized milk samples by developed chromogenic and fluorogenic assay method were in accordance with the conventional IS methods. Conclusion: Developed chromogenic and fluorogenic assay method indicating their suitability, ease in operation, time saving and preciseness over the conventional methods and can be opt as a suitable alternative for monitoring presence of E. coli and coliforms in fluid milks.

show abstract

“…In some studies on this subject, Rice et al (1990) reported that E. coli strains isolated from a total of 460 human, 105 cows and 55 horses were screened and 95.5% β-glucuronidase-positive were isolates in 24 h and 99.5% positive after 28 h of incubation. Doğan and Halkman (1998) isolated 190 E. coli strains in the studies and they reported that all colonies gave the fluorescent positive in LST + MUG media. In our study, it was also confirmed that a total of 40 isolates were consisted of E. coli species according to biochemical characteristics and β-glucuronidase activities.…”

Section: Discussionmentioning

confidence: 99%

Investigating to Some Microbial Pollution Parameters of Seawater and Mussels (Mytilus galloprovincialis, Lamarck 1819) of Sіnop Black Sea Coastal Zone, Turkey

Avşar¹,

Berber²

2014

JSM

View full text Add to dashboard Cite

The presence of coliforms bacteria is one of the most prevalent problems in terms of public health in marine ecosystems over the world. In this study were investigated the physico-chemical properties of seawater and the numbers of total aerobic, total coliform, fecal coliform, E. coli O157:H7 and fecal streptococci in seawater and mussel samples collected from Sinop environs between May and October 2011. The microbiological analysis of seawater samples showed that the difference between total coliform, fecal coliform and fecal streptococci numbers (p<0.05) was significant for each station. However, the difference among total aerobic bacteria numbers for each stations (p>0.05) were not found significant. The difference between whole counting results for mussel samples taken from different sampling sites was not significant (p>0.05), too. Furthermore, the results of the screening assay for the presence of E. coli O157:H7 showed that the strain was not detected in neither seawater nor mussel samples. In conclusion, it was determined that fecal coliform and fecal streptococci counts in the seawater and mussel samples were higher than legal (Turkish Bathing Water and Quality of Fishery Products Regulation) limit values for some stations in Sinop coastal areas.

show abstract

Comparison of LST + MUG broth technique and conventional method for the enumeration of Escherichia coli in foods

Cited by 4 publications

References 18 publications

Bedding and Seasonal Effects on Chemical and Bacterial Properties of Feedlot Cattle Manure

Bedding and Seasonal Effects on Chemical and Bacterial Properties of Feedlot Cattle Manure

Comparison of Standardized X-gal, ONPG and MUG Assay Methods with IS Methods by Analyzing Milk Samples

Investigating to Some Microbial Pollution Parameters of Seawater and Mussels (Mytilus galloprovincialis, Lamarck 1819) of Sіnop Black Sea Coastal Zone, Turkey

Contact Info

Product

Resources

About