Comparison of Lumbar Spine Fusion Using Mixed and Cloned Marrow Cells

Cui, Quanjun; Xiao, Zeng; Balian, Gary; Wang, Gwo-Jaw

doi:10.1097/00007632-200111010-00003

Cited by 62 publications

(33 citation statements)

References 42 publications

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“…Moreover, previously published rat spinal fusion studies assessing autologous bone grafting were observed to yield an inferior fusion rate when compared with hPSC treatment (0% fusion after 4 weeks and 11% fusion after 8 weeks with autologous bone grafting, compared with 80%-100% fusion in 4 weeks with hPSC treatment) [46,47]. MSCs derived from multiple sources such as bone marrow and adipose tissue have attracted large interest for replacement of autogenous bone in spinal fusion procedures [48][49][50][51][52]. BMSCs, however, are limited in supply, and it is estimated that only 1 BMSC can be isolated per 100,000 cells [53].…”

Section: Discussionmentioning

confidence: 99%

“…BMSCs, however, are limited in supply, and it is estimated that only 1 BMSC can be isolated per 100,000 cells [53]. To circumvent the shortage of MSCs, readily isolatable methods such as selective cell retention [54] and ex vivo cell culture methods have been developed [48][49][50]. Selective cell retention of osteoprogenitors are clinically available using Cellect Graft Preparation system (Dupuy, Raynham, MA, http://www.depuy.com); however, no clinical reports of success have been published [55].…”

Section: Discussionmentioning

confidence: 99%

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Human Perivascular Stem Cell-Based Bone Graft Substitute Induces Rat Spinal Fusion

Chung

James

Asatrian

et al. 2015

Stem Cells Translational Medicine

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Adipose tissue is an attractive source of mesenchymal stem cells (MSCs) because of its abundance and accessibility. We have previously defined a population of native MSCs termed perivascular stem cells (PSCs), purified from diverse human tissues, including adipose tissue. Human PSCs (hPSCs) are a bipartite cell population composed of pericytes (CD146+CD342CD452) and adventitial cells (CD1462CD34+ CD452), isolated by fluorescence-activated cell sorting and with properties identical to those of culture identified MSCs. Our previous studies showed that hPSCs exhibit improved bone formation compared with a sample-matched unpurified population (termed stromal vascular fraction); however, it is not known whether hPSCs would be efficacious in a spinal fusion model. To investigate, we evaluated the osteogenic potential of freshly sorted hPSCs without culture expansion and differentiation in a rat model of posterolateral lumbar spinal fusion. We compared increasing dosages of implanted hPSCs to assess for dose-dependent efficacy. All hPSC treatment groups induced successful spinal fusion, assessed by manual palpation and microcomputed tomography. Computerized biomechanical simulation (finite element analysis) further demonstrated bone fusion with hPSC treatment. Histological analyses showed robust endochondral ossification in hPSC-treated samples. Finally, we confirmed that implanted hPSCs indeed differentiated into osteoblasts and osteocytes; however, the majority of the new bone formation was of host origin. These results suggest that implanted hPSCs positively regulate bone formation via direct and paracrine mechanisms. In summary, hPSCs are a readily available MSC population that effectively forms bone without requirements for culture or predifferentiation. Thus, hPSC-based products show promise for future efforts in clinical bone regeneration and repair. STEM CELLS

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Human Perivascular Stem Cell-Based Bone Graft Substitute Induces Rat Spinal Fusion

Chung

James

Asatrian

et al. 2015

Stem Cells Translational Medicine

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“…Mesenchymal stem cells have been used in multiple tissue-engineering applications, and they differentiate into multiple lineages, including osteoblasts, chondrocytes, adipocytes, and muscle/tendon forming cells [9][10][11]23]. The multipotent, immortalized BMSC line used here is primarily osteogenic [24] and has been used in models of spinal fusion [19] and in the femur [22]. These cells also differentiate into adipocytes under the influence of steroids [20,21] or alcohol [22].…”

Section: Discussionmentioning

confidence: 99%

The Effects of GDF-5 and Uniaxial Strain on Mesenchymal Stem Cells in 3-D Culture

Farng

Urdaneta²,

Barba³

et al. 2008

Clin Orthop Relat Res

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Recent endeavors in tissue engineering have attempted to identify the optimal parameters to create an artificial ligament. Both mechanical and biochemical stimulation have been used by others to independently modulate growth and differentiation, although few studies have explored their interactions. We applied previously described fabrication techniques to create a highly porous (90%-95% porosity, 212-300 lm), 3-D, bioabsorbable polymer scaffold (polycaprolactone). Scaffolds were coated with bovine collagen, and growth and differentiation factor 5 (GDF-5) was added to half of the scaffolds. Scaffolds were seeded with mesenchymal stem cells and cultured in a custom bioreactor under static or cyclic strain (10% strain, 0.33 Hz) conditions. After 48 hours, both mechanical stimulation and GDF-5 increased mRNA production of collagen I, II, and scleraxis compared to control; tenascin C production was not increased. Combining stimuli did not change gene expression; however, cellular metabolism was 1.7 times higher in scaffolds treated with both stimuli. We successfully grew a line of mesenchymal stem cells in 3-D culture, and our initial data indicate mechanical stimulation and GDF-5 influenced cellular activity and mRNA production; we did not, however, observe additive synergism with the mechanical and biological stimuli.

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“…Bone marrow stem cells could serve as graft material and a vehicle to deliver therapeutic factors that enhance bone repair [9,13]. The efficacy of bone marrow implantation in the osteonecrotic zone was studied in prospective trials in humans and showed progression of the disease was avoided in nine of the 10 hips that received bone marrow implantation, whereas five of the eight hips in the control group had deteriorated to a late stage of the disease [14][15][16].…”

Section: Next Stepsmentioning

confidence: 99%

Emerging Ideas: Treatment of Precollapse Osteonecrosis Using Stem Cells and Growth Factors

Cui

Botchwey

2011

Clinical Orthopaedics &Amp; Related Research

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Comparison of Lumbar Spine Fusion Using Mixed and Cloned Marrow Cells

Abstract: Compared with mixed marrow stromal cells, cloned osteoprogenitor cells can produce a larger amount of mature osseous tissue at an earlier time point during spine fusion.

Cited by 62 publications

References 42 publications

Human Perivascular Stem Cell-Based Bone Graft Substitute Induces Rat Spinal Fusion

Human Perivascular Stem Cell-Based Bone Graft Substitute Induces Rat Spinal Fusion

The Effects of GDF-5 and Uniaxial Strain on Mesenchymal Stem Cells in 3-D Culture

Emerging Ideas: Treatment of Precollapse Osteonecrosis Using Stem Cells and Growth Factors

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