Comparison of MB/BacT ALERT 3D System with Radiometric BACTEC System and Löwenstein-Jensen Medium for Recovery and Identification of Mycobacteria from Clinical Specimens: a Multicenter Study

Piersimoni, Claudio; Scarparo, Claudio; Callegaro, Annapaola; Tosi, C; Nista, Domenico; Bornigia, Stefano; Scagnelli, Mariuccia; Rigon, Alessandra; Ruggiero, Giuliana; Goglio, Antonio

doi:10.1128/jcm.39.2.651-657.2001

Cited by 85 publications

(50 citation statements)

References 22 publications

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“…The sediment of the centrifuged CSF was added to 50 µl rates of 91% to 94% of BacT/ALERT [16][17]. The contamination rates of BacT/ALERT and the LJ culture in our study were found to be 5.8% and 7.2% respectively.…”

Section: Possible Tbm: (I) Clinical Criteria (A)mentioning

confidence: 47%

The Diagnostic Utility of Bact/ALERT and Nested PCR in the Diagnosis of Tuberculous Meningitis

Sastry¹

2013

JCDR

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Objective: The early laboratory diagnosis of Tuberculous Meningitis (TBM) is crucial, to start the antitubercular chemotherapy and to prevent its complications. However, the conventional methods are either less sensitive or time consuming. Hence, the diagnostic potentials of BacT/ALERT and Polymerase Chain Reaction (PCR) was evaluated in this study. Material and Method:The study group comprised of 62 cases and 33 controls. The cases were divided according to Ahuja's criteria into the confirmed (two cases), highly probable (19 cases), probable (26 cases) and the possible (15 cases) subgroups. Ziehl Neelsen's (ZN) and Auramine Phenol (AP) staining, Lowenstein Jensen (LJ) medium culture, BacT/ALERT and nested Polymerase Chain Reaction (PCR) which targeted IS6110 were carried out on all the patients. Observation and Results:The sensitivity of the LJ culture was 3.22%. BacT/ALERT showed a sensitivity and a specificity of 25.80% and 100% and those of nested PCR were found to be 40.32% and 96.97% respectively. The mean detection time of growth of the LJ culture was 31.28 days, whereas that of BacT/ALERT was 20.68 days. The contamination rate in the LJ culture and BacT/ALERT were 7.2% and 5.8% respectively. Conclusion:Nested PCR was found to be more sensitive, followed by BacT/ALERT as compared to the LJ culture and smear microscopy. As both false negative and false positive results have been reported for nested PCR, so it should not be used alone as a criterion for initiating or terminating the therapy, but it should be supported by clinical, radiological, cytological and other microbiological findings.

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Section: Possible Tbm: (I) Clinical Criteria (A)mentioning

confidence: 47%

The Diagnostic Utility of Bact/ALERT and Nested PCR in the Diagnosis of Tuberculous Meningitis

Sastry¹

2013

JCDR

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“…Each method has its own advantages and disadvantages, starting from the LJ medium and the Middlebrook 7H 10 medium (MB7H10) to the present trendy and speedy automated methods like the MB/BACT 3D device [8][9][10][11][12][13]. MB/BACT is a safer and a quicker method as it is an automated method which involves liquid media and as it does not involve any radioactive material.…”

Section: Resultsmentioning

confidence: 99%

“…Damle and Kaundinya found that the Nassau's swab method of decontamination was superior to Pettroff's method and the NALC method in giving positive cultures. The study of Claudio Peirsiomoni et al, has also reported a variation in the culture yield when different decontamination methods were used [25]. We included the modified Petroff's method in our study.The previous antitubercular treatment and its duration is an important determining factor for the isolation of MTB.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens

Naveen¹,

Peerapur²

2012

JCDR

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Introduction: Tuberculosis (TB) is the most common cause of death due to a single infectious agent worldwide in adults. India alone accounts for 30% of the global tuberculosis burden. There is a need for a method of cultivation of mycobacteria that is reliable and economical and has a short turnaround time.Objective: The present study was attempted to assess the feasibility of using MB BACT and Middlebrook 7h10(MB7H10) as primary isolation media for mycobacteria. They were compared with the LJ medium, which was the gold standard. Materials and Methods:Various clinical specimens from a total of 236 clinically suspected cases of TB were studied. All the samples were decontaminated by using the modified Petroff's method. Each sample was subjected to ZN staining and it was simultaneously inoculated onto the LJ medium, the MB7H10 medium and MB BACT. The growth from the cultures were confirmed by ZN staining and they were speciated by using biochemical reactions.Results: Out of the 236 samples which were screened, 116 isolates were obtained. All the 116 were isolated from MB BACT, 82 were isolated from the LJ medium and 62 were isolated from MB7H10. 82 isolates were obtained from MB BACT and the LJ medium, 62 were obtained from MB7H10 and MB BACT, 58 were isolated from LJ and MB7H10 and 58 were isolated from LJ medium, MB7H10 and MB bact. Neither the L J medium nor the Middlebrook 7h 10 medium could isolate mycobacteria exclusively. It showed that the combination of media did not prove to be superior over the use of MB BACT alone. The average isolation time of L J, the MB7H10 medium and MB BACT was 30

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“…Prior studies have demonstrated higher bacterial contami- on April 7, 2019 by guest http://jcm.asm.org/ nation rates with predominantly gram-positive organisms in the MB/BacT system than with other methods, despite the addition of vancomycin to the MAS (1,2,6,8,11,12). This study evaluated the MAS concentration of vancomycin and compared the manufacturer's recommended protocol with another method in an effort to decrease bacterial contamination in the MB/BacT system.…”

mentioning

confidence: 98%

Methods for Reducing Bacterial Contamination in the BacT/Alert Mycobacterial Culture Detection System

et al. 2005

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Two methods were compared for decreasing bacterial contamination in the BacT/Alert mycobacterial culture detection system. Two concentrations, 0.5 ml (standard amount) and 1.0 ml, of mycobacterial antibiotic supplement were evaluated. Contamination rates were 14% and 6% for the standard and the doubled concentrations, respectively. This difference was statistically significant (P < 0.005).Digestion and decontamination of clinical specimens from nonsterile body sites is required for optimal recovery of mycobacterial species, which can be quickly overgrown by contaminating bacteria. For this reason, commercial broth culture systems utilize lyophilized antibiotics, which are reconstituted and used for supplementation of standard broth media to decrease bacterial contamination.Our laboratory experienced two 1-month episodes of increased bacterial contamination in less than a year following implementation of the BacT/Alert mycobacterial culture detection system (MB/BacT system; bioMerieux, Durham, N.C.). The interval between these events was approximately 4 months. These rates were 23.4% (27/115) and 17.9% (41/229). Similar contamination was not seen in the corresponding solid media cultures (4.5% and 13.5%, respectively). Gram-positive cocci predominated among the contaminants, leading us to question the concentration and stability of the gram-positive active agents in the mycobacterial antibiotic supplement (MAS). The manufacturer states that the concentration of vancomycin in the reconstituted MAS vials should be 5.0 g/ml. The goals of this study were (i) to evaluate the concentration of grampositive antimicrobials, specifically vancomycin, in the MAS by using both analytical and bioassay methods and (ii) to compare the manufacturer's recommended protocol (0.5 ml MAS) with an alternative method (1.0 ml MAS) to decrease bacterial contamination in both seeded and clinical specimens (n ϭ 100) in the MB/BacT system. Digestion and decontamination of study specimens was performed using standard methods (7) and a commercially available reagent containing N-acetyl-L-cysteine (0.375 gm), trisodium citrate (0.1 M), and sodium hydroxide (4%) (Scientific Device Laboratories, Des Plaines, Illinois). The following were added to individual BacT/Alert mycobacteria processing (MP) bottles: the standard amount (0.5 ml) of MAS as recommended by the manufacturer and twice the standard amount of MAS (1.0 ml) according to a validated protocol from the Arkansas Department of Health (S. F. Tidwell and T. Clark, Abstr. 98th Gen. Meet. Am. Soc. Microbiol. 1998Microbiol. , abstr. A-34, p. 43, 1998. This protocol differs from the standard method only in the amount of MAS used. Mycobacteria recovered from positive cultures were identified using standard methods (5, 7, 10). Seeded MP bottles were inoculated with approximately 10 5 CFU/ml for each of the following mycobacterial species: Mycobacterium xenopi, M. asiaticum, M. mucogenicum, M. chelonae, M. abscessus, and M. tuberculosis. These mycobacterial species were selected for "seeding" ex...

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Comparison of MB/BacT ALERT 3D System with Radiometric BACTEC System and Löwenstein-Jensen Medium for Recovery and Identification of Mycobacteria from Clinical Specimens: a Multicenter Study

Cited by 85 publications

References 22 publications

The Diagnostic Utility of Bact/ALERT and Nested PCR in the Diagnosis of Tuberculous Meningitis

The Diagnostic Utility of Bact/ALERT and Nested PCR in the Diagnosis of Tuberculous Meningitis

Comparison of the Lowenstein-Jensen Medium, the Middlebrook 7H10 Medium and MB/BacT for the Isolation of Mycobacterium Tuberculosis (MTB) from Clinical Specimens

Methods for Reducing Bacterial Contamination in the BacT/Alert Mycobacterial Culture Detection System

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