Comparison of methionine sources around requirement levels using a methionine efficacy method in 0 to 28 day old broilers

Abstract

The addition of methionine in the poultry feed industry is still facing the relative efficacy dilemma between DL-methionine (DLM) and hydroxy-methionine (HMTBA). The aim of this study was to compare the effect of dietary DLM and HMTBA on broiler performance at different levels of total sulfur amino acids (TSAA). The treatments consisted of a basal diet without methionine addition, and 4 increasing methionine doses for both sources resulting in TSAA/Lysine ratios from 0.62 to 0.73 in the starter phase and 0.59 to 0.82 in the grower phase. The comparison of product performance was performed by three-way ANOVA analysis and by methionine efficacy calculation as an alternative method of comparison. Growth results obtained during the starter phase with the different methionine supplementations did not show significant growth responses to TSAA levels, indicating a lower methionine requirement in the starter phase than currently assumed. However, a significant methionine dose effect was obtained for the period 10 to 28 day of age and for the entire growth period of 0 to 28 day of age. Excepting a significant gender effect, the statistical analysis did not allow for the discrimination of methionine sources, and no interaction between source and dose level was observed up to 28 days of age. A significant interaction between source and dose level was observed for methionine efficacy for the grower phase, and the total growth period showed better HMTBA efficacy at higher TSAA value. The exponential model fitted to each methionine source for body weight response depending on methionine intake or for feed conversion ratio (FCR) depending on methionine doses did not allow the methionine sources to be distinguished. Altogether, these results conclude that methionine sources lead to similar performances response when compared at TSAA values around the broiler requirement level. These results also showed that at TSAA values above requirement, HMTBA had a better methionine efficacy value than DLM, caused by the different properties of that molecule, whereas below the TSAA requirement levels, the opposite was observed in females.

Improving pork quality traits by a short-term dietary hydroxy methionine supplementation at levels above growth requirements in finisher pigs

“…Calculated from the calculated ileal digestibility values of the ingredients, and adjusted for 86.3% of dry matter. e Analysed methionine equivalent (Total Met + HMTBA) and cystine levels (Agostini et al, 2015) A C C E P T E D M A N U S C R I P T 60.1 60.6 60.5 1.9 0.86 a Pigs were fed either a control diet (CONT) adequate in Met or a HMTBA-supplemented diets with calculated value of Met being respectively, 3 times (Met3 group) or 5 times greater (Met5 group), than that in the CONT diet. Results are presented as means for the 3 dietary treatments (n = 15 pigs/group) and Residual standard deviation (Rsd) of the analysis of variance.…”

Antioxidant response and bioavailability of methionine hydroxy analog relative to DL-methionine in broiler chickens

“…DL-methionine (DLM) and DL-2-hydroxy-4-methylthio-butanoic acid (HMTBa) are 2 commonly used Met additives (Agostini et al., 2015). DL-methionine and HMTBa are structurally different molecules with different physiological characteristics (Tang et al., 2011, Zhang et al., 2016), and have different optimum dietary levels in poultry.…”

Effect of 2‐hydroxy‐4‐(methylthio) butanoic acid and acidifier on the performance, chyme pH, and microbiota of broilers

“…The dose-response curve with the best goodness of fit was linear for HMTBA and quadratic for DLM (Vazquez-Anon et al, 2006). Therefore, HMTBA is suggested to outperform DLM when TSAA values are above requirement (Agostini et al, 2016;Vazquez-Anon et al, 2006 Values with different superscripts in the same column differ significantly (p < .05).…”