2021
DOI: 10.1186/s13068-021-01897-y
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of methodologies used to determine aromatic lignin unit ratios in lignocellulosic biomass

Abstract: Background Multiple analytical methods have been developed to determine the ratios of aromatic lignin units, particularly the syringyl/guaiacyl (S/G) ratio, of lignin biopolymers in plant cell walls. Chemical degradation methods such as thioacidolysis produce aromatic lignin units that are released from certain linkages and may induce chemical changes rendering it difficult to distinguish and determine the source of specific aromatic lignin units released, as is the case with nitrobenzene oxida… Show more

Help me understand this report
View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
15
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 27 publications
(18 citation statements)
references
References 79 publications
3
15
0
Order By: Relevance
“…There is a paradox between the simplicity of monolignol basic units and the complexity of the polymerized network, which is a result of the variety of linkages present in lignin. , Solution NMR is the most employed analytical technique to characterize lignin (Figure A), ,,, and only a few ssNMR experiments have been carried out to investigate the lignin structure. 1D ssNMR approaches have been used to determine how well lignin fractions were solubilized prior to solution NMR measurements and to avoid the chemically destructive acidic hydrolysis methods (like Klason lignin analysis) for quantification. Efforts have also been made to estimate the ratio between the G and S units in poplar and other woody plants, but insufficient resolution prevents accurate determination of the concentration of individual lignin units and linkage patterns . In addition, all deconvolution procedures applied in the spectral analysis are affected by considerable uncertainty, especially when broad peaks overlap (Figure B).…”
Section: Solid-state Nmr Studies Of Plant Materialsmentioning
confidence: 99%
See 1 more Smart Citation
“…There is a paradox between the simplicity of monolignol basic units and the complexity of the polymerized network, which is a result of the variety of linkages present in lignin. , Solution NMR is the most employed analytical technique to characterize lignin (Figure A), ,,, and only a few ssNMR experiments have been carried out to investigate the lignin structure. 1D ssNMR approaches have been used to determine how well lignin fractions were solubilized prior to solution NMR measurements and to avoid the chemically destructive acidic hydrolysis methods (like Klason lignin analysis) for quantification. Efforts have also been made to estimate the ratio between the G and S units in poplar and other woody plants, but insufficient resolution prevents accurate determination of the concentration of individual lignin units and linkage patterns . In addition, all deconvolution procedures applied in the spectral analysis are affected by considerable uncertainty, especially when broad peaks overlap (Figure B).…”
Section: Solid-state Nmr Studies Of Plant Materialsmentioning
confidence: 99%
“…In addition, all deconvolution procedures applied in the spectral analysis are affected by considerable uncertainty, especially when broad peaks overlap (Figure B). Lignin units can be better resolved in 2D 13 C– 13 C correlation spectra (Figure C). , DNP has also been applied to wild-type and mutant poplar samples to unravel the structural changes of lignin, and the resolution enabled distinguishing the three types of fundamental units and partially probing their linkages . More efforts are needed to improve the ssNMR capability for lignin structure characterization to par with solution NMR methods, with the additional merit of using native samples free of chemical treatment, ball-milling (which restructures biomolecules, as shown recently), and solubilization procedures.…”
Section: Solid-state Nmr Studies Of Plant Materialsmentioning
confidence: 99%
“…In contrast, lignin content was higher in our study compared with a previous analysis of S. trifasciata fibers that showed that Klason lignin made up 6% of the total dry mass 34 , about 42% lower than we found by py-MBMS. The differences in lignin composition may be due to differences in the type of lignin quantified in each technique (Klason lignin, acid-insoluble lignin; pyMBMS, total lignin) and sensitivity of the methods used 37 . For Sansevieria fibers, our report is the first to determine the chemical composition using high performance chromatography and mass spectroscopy.…”
Section: Resultsmentioning
confidence: 99%
“…Pseudo-Voigt peak shapes can be viewed as a convolution of a Gaussian and Lorentzian peak shape. These peak shapes are particularly fit to resemble spectral peaks from NMR . Indeed, the natural line shapes from NMR have a Lorentzian profile, while, e.g., apodization using commonly used window functions, such as the 2π-Kaiser window function, adds a Gaussian component.…”
Section: Methods Development and Discussionmentioning
confidence: 99%