Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

Abstract

Sansevieria trifasciata is used as an indoor plant, in traditional medicine and as a fiber source. Here we characterized fibers of two of varieties of S. trifasciata, Lorentii and Hahnii, and report a protocol for their propagation based on indirect shoot organogenesis. Structural and ribbon fibers were scattered within leaf parenchyma when viewed with confocal laser scanning microscopy. Chemical analysis of the fibers by mass spectrometry and high-performance chromatography revealed higher contents of cellulose and xylose in Lorentii than in Hahnii and significant differences for total lignin between both. A protocol for de novo shoot production was then developed using leaf explants. Time-course histological analyses showed that the first events of transdifferentiation were triggered preferentially in cells surrounding fibers and vascular bundles. Callogenesis and shoot performances were quantified for both varieties, and 2,4-D at 2 and 3 mg·L-1 yielded the best results for primary calli induction and fresh calli mass. The length, number, and mass of shoots produced did not differ significantly between the two cultivars. The fast morphogenic response of S. trifasciata to in vitro culture may be useful for mass propagation or other biotechnological purposes such as metabolite production.

Somatic Embryogenesis and Flow Cytometric Assessment of Nuclear Genetic Stability for Sansevieria spp.: An Approach for In Vitro Regeneration of Ornamental Plants

“…Mass propagation through in vitro procedures has been investigated as a solution to address the increasing commercial demand. Recently, direct and indirect organogenesis from leaf explants have been reported for S. trifasciata and S. masoniana [3,[10][11][12]. Here we report for the first time, to the best of our knowledge, a technique for plant regeneration starting from flower explants.…”

“…is a genus of succulent plants with approximately 90 distinct species identified [1], originally found in tropical central Africa and drier parts of eastern and southern Africa [2]. Sansevieria plants are cultivated for medicinal, fodder, soil conservation and fiber uses [3]. Recently it has been reported that Sansevieria plants have also a high capacity to reduce environmental pollution produced by gases and heavy metals thanks to the ability to absorb hazardous pollutants such as VOCs and CO 2 emissions [4,5].…”

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

“…A histological analysis of the chronological sequence of morphological events during ontogeny is a way to know what morphogenic process was induced (Delporte et al, 2014). Somatic embryogenesis and/or organogenesis has been described and confirmed using histological analysis in different species (Delgado-Aceves et al, 2021;Mamgain et al, 2022;García-Hernández et al, 2022).…”

An exopolysaccharide-producing novel Agrobacterium pusense strain JAS1 isolated from snake plant enhances plant growth and soil water retention

“…Other investigational leads explore interactions with microbionts which variously augment resilience attributes in these plants 82 – 85 . Research in the genus Sansevieria however witnesses a slow pace in current developments majoring only with its phytochemical 73 – 75 and fiber reserves 86 . Recent literature depicts a recessed trend of investigations especially those warranted on various stressors and mechanisms that allow survivability of Sansevieria plants.…”