Comparison of methods for detection of four common nosocomial pathogens on hospital textiles

Fijan, Sabina; Turk, Sonja Šoštar; Rozman, Urška

doi:10.2478/sjph-2014-0003

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…The non-destructive elution method using the Morapex A device (Mathis AG, Switzerland) 33 was used to retrieve the bacteria from the textiles. This method has already been proven to be an efficient replacement of the simple vortexing method with saline solution and added Tween 80.…”

Section: Methodsmentioning

confidence: 99%

“…This method has already been proven to be an efficient replacement of the simple vortexing method with saline solution and added Tween 80. 33,34 Briefly, 20 mL of test liquid containing 0.9% NaCl and 0.2% solution of polysorbate (Tween 80, Sigma-Aldrich, Germany) was pressed through the textile swatch at a cycle of 15 s. This cycle was conducted three times. The extract was then collected in a test tube.…”

Section: Methodsmentioning

confidence: 99%

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Survival of Enterococcus faecium, Staphylococcus aureus and Pseudomonas aeruginosa on cotton

Fijan

Pahor

Turk

2016

Textile Research Journal

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The aim of this study was to evaluate the survival of three challenge bacteria stored at 25℃, 5℃, and 50℃ up to 21 days on 100% cotton textile swatches. The survival of each chosen challenge bacteria was determined by the classical phenotype colony counting method through growth on selective media, and the molecular method of detecting Staphylococcus aureus-, Enterococcus faecium-, and Pseudomonas aeruginosa-specific DNA. E. faecium proved to be the most persistent chosen challenge bacteria at all three chosen storage temperatures. Both E. faecium and S. aureus survived on textile swatches up to 21 days at 25℃ at the highest inoculum. E. faecium and P. aeruginosa persisted at 50℃ only up to 9 and 3 days, respectively. All challenge bacteria survived on textile swatches at 5℃ ( E. faecium 12 days, P. aeruginosa and S. aureus less than 6 days). The results of survival at lower initial inoculum were lower and less dependent on temperature. Dehydration phenomena or fluctuation in relative humidity in the refrigerator at 5℃ possibly explains lower survival than at room temperature. Positive results for the detection of E. faecium- and P. aeruginosa-specific DNA were found for some samples with negative results for classical phenotype counting methods, perhaps owing to the fact that the bacterial cells, due to adverse environmental conditions, reached a viable, but noncultivable (VBNC) state, or that the extracellular DNA fragments persisted on the textiles. Survival of the challenge bacteria for more than 3 days regardless of temperature proves that hospital textiles as an inanimate surface should not be neglected as one of the possible vectors for transmission of pathogens causing healthcare associated infections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Survival of Enterococcus faecium, Staphylococcus aureus and Pseudomonas aeruginosa on cotton

Fijan

Pahor

Turk

2016

Textile Research Journal

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“…Although the swabbing technique is widely used, it lacks the standardized conditions necessary for reproducible results [15]. Currently, studies on the recovery of bacteria using both swab and contact plate sampling methods are primarily conducted in controlled laboratory environments with single or multiple bacterial species [15,[19][20][21]. However, in real medical environments, the bacterial community consists of various bacteria originating from soil and skin surfaces and other organic matter [22].…”

Section: According To the 2022 Who Global Report On Infection Prevent...mentioning

confidence: 99%

Comparative Performance of Contact Plate Metod and Swab Method for Surface Microbial Contamination on Medical Fabrics

Chen,

Li,

Wang

et al. 2023

Preprint

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Background: The contact plate method is widely accepted and used in various fields where hygiene and contamination levels are crucial. Evidence regarding the applicability of the contact plate method for sampling fabric microbial contamination levels in real medical environments was limited.This study aimed to assess the applicability of the contact plate method for detecting microbial contamination on medical fabrics in a real healthcare environment, thereby providing a benchmark for fabric microbial sampling methods. Methods: In a level three obstetrics ward of a hospital, twenty-four privacy curtains adjacent to patient beds were selected for this study. The contact plate and cotton swab method were used to collect microbial samples from the curtains on the 1st, 7th, 14th, and 28th days after they were hung. The total colony count on each curtain surface was calculated, and microbial identification was performed. Results: After excluding the effects of time, room type, and curtain location on the detected microbial load, the linear mixed-effects model analysis showed that the contact plate method yielded lower colony counts compared to the cotton swab method (P<0.001). However, the contact plate method isolated more microbial species than the cotton swab method (P <0.001). The predominant microbial species isolated from both methods were gram-positive bacteria (78.22%), followed by fungi (14.05%). The top three detected bacteria were Staphylococcus epidermidis (24.59%), Micrococcus luteus (23.42%), and Staphylococcus hominis (21.08%). Furthermore, the microbial load on curtains in double-occupancy rooms was lower than those in triple-occupancy rooms (P = 0.021), and the microbial load on curtains near windows was lower than that near doors (P = 0.004). Conclusion: The results showed that the contact plate sampling technique is more suitable for investigating hospital infection outbreaks and identifying specific pathogens on contaminated medical fabrics than the cotton swab method.

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“…Although the swabbing technique is widely used, it lacks the standardized conditions necessary for reproducible results [ 15 ]. Currently, studies on the recovery of bacteria using both swab and contact plate sampling methods are primarily conducted in controlled laboratory environments with single or multiple bacterial species [ 15 , 19 – 21 ]. However, in real medical environments, the bacterial community consists of various bacteria originating from soil and skin surfaces and other organic matter [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative performance of contact plate metod and swab method for surface microbial contamination on medical fabrics

Chen,

Li,

Wang

et al. 2024

BMC Infect Dis

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Background The contact plate method is widely accepted and used in various fields where hygiene and contamination levels are crucial. Evidence regarding the applicability of the contact plate method for sampling fabric microbial contamination levels in real medical environments was limited. This study aimed to assess the applicability of the contact plate method for detecting microbial contamination on medical fabrics in a real healthcare environment, thereby providing a benchmark for fabric microbial sampling methods. Methods In a level three obstetrics ward of a hospital, twenty-four privacy curtains adjacent to patient beds were selected for this study. The contact plate and swab method were used to collect microbial samples from the privacy curtains on the 1st, 7th, 14th, and 28th days after they were hung. The total colony count on each privacy curtain surface was calculated, and microbial identification was performed. Results After excluding the effects of time, room type, and curtain location on the detected microbial load, the linear mixed-effects model analysis showed that contact plate method yielded lower colony counts compared to swab method (P < 0.001). However, the contact plate method isolated more microbial species than swab method (P < 0.001). 291 pathogenic strains were isolated using the contact plate method and 133 pathogenic strains were isolated via the swab method. There was no difference between the two sampling methods in the detection of gram-negative bacteria (P = 0.089). Furthermore, the microbial load on curtains in double-occupancy rooms was lower than those in triple-occupancy rooms (P = 0.021), and the microbial load on curtains near windows was lower than that near doors (P = 0.004). Conclusion Contact plate method is superior to swab method in strain isolation. Swab method is more suitable for evaluating the bacterial contamination of fabrics.

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Comparison of methods for detection of four common nosocomial pathogens on hospital textiles

Abstract: Introduction: Although the most common vehicle for transmission of health-care acquired infections is the personto-person transmission route, the role of environment should not be ignored and hospital linen may contribute to

Cited by 6 publications

References 36 publications

Survival of Enterococcus faecium, Staphylococcus aureus and Pseudomonas aeruginosa on cotton

Survival of Enterococcus faecium, Staphylococcus aureus and Pseudomonas aeruginosa on cotton

Comparative Performance of Contact Plate Metod and Swab Method for Surface Microbial Contamination on Medical Fabrics

Comparative performance of contact plate metod and swab method for surface microbial contamination on medical fabrics

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