Comparison of methods for erythroblast selection: Application to selecting fetal erythroblasts from maternal blood

Collarini, Ellen J.; Cain, Carol A.; Gammon, Dawn; Harriman, Bill; Magee, Kerry; Du, Gehua; Schueler, Paula A.; Mahoney, Walt

doi:10.1002/1097-0320(20011201)45:4<267::aid-cyto10023>3.0.co;2-d

Cited by 13 publications

(12 citation statements)

References 40 publications

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“…Chaotic trajectories of the magnetic particles, generated through appropriate temporal variations of electric currents in microconductors imbedded in microchannel walls, have been employed to enhance the mixing of particles and cells (68). Ferrofluid-based capture reagents consisting of nanoparticles with a magnetic core surrounded by a polymeric layer coated with antibodies have also been reported for cell-separation applications (69). Limitations in the purity of the final product and efficiency of the separation steps can be largely eliminated by the use of microscale devices.…”

Section: Microfluidic Flow Cell Sortersmentioning

confidence: 99%

Blood-on-a-Chip

Toner

Irimia²

2005

Annu. Rev. Biomed. Eng.

590

451

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Accurate, fast, and affordable analysis of the cellular component of blood is of prime interest for medicine and research. Yet, most often sample preparation procedures for blood analysis involve handling steps prone to introducing artifacts, whereas analysis methods commonly require skilled technicians and well-equipped, expensive laboratories. Developing more gentle protocols and affordable instruments for specific blood analysis tasks is becoming possible through the recent progress in the area of microfluidics and lab-on-a-chip-type devices. Precise control over the cell microenvironment during separation procedures and the ability to scale down the analysis to very small volumes of blood are among the most attractive capabilities of the new approaches. Here we review some of the emerging principles for manipulating blood cells at microscale and promising high-throughput approaches to blood cell separation using microdevices. Examples of specific single-purpose devices are described together with integration strategies for blood cell separation and analysis modules.

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Section: Microfluidic Flow Cell Sortersmentioning

confidence: 99%

Blood-on-a-Chip

Toner

Irimia²

2005

Annu. Rev. Biomed. Eng.

590

451

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“…A selection from 10 ml of blood using a subsaturation antibody concentration yields about 10 6 -3 ϫ 10 7 cells in the positive fraction (9). This number of cells would require 5-150 cytospins, at a cost of approximately $8 -$249 per sample in consumables.…”

Section: Resultsmentioning

confidence: 99%

“…The same number of cells could be placed on one to six flaskettes at a lower cost of approximately $5-$31 per sample in comsumables (estimates are based on current, nondiscounted prices). With a sample that results in a large number of positive cells, the time and effort needed to prepare six flaskettes is much less than that needed to prepare 150 precoated cytospins (9).…”

Section: Resultsmentioning

confidence: 99%

A novel method for depositing erythroid cells onto glass slides for fetal cell analysis

Collarini¹,

Nagy²,

Cain³

et al. 2001

Cytometry

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“…The first reliable report performed on a pre-CVS sample estimated around 1 cell per mL maternal blood [3]. Later reports estimate between 1–5 cells per mL maternal blood [15, 37, 38], depending of the method of enrichment. Others have however found foetal NRBC at much lower frequencies [39–41].…”

Section: Discussionmentioning

confidence: 99%

Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis

Sørensen

Dosal

Jensen

et al. 2009

BioMed Research International

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Invasive procedures for prenatal diagnosis are associated with increased risk of abortion; thus, development of noninvasive procedures would be beneficial. Based on the observation that embryonic nucleated red blood cell (NRBC) crosses the placenta and enters the circulation of pregnant women, the ability to identify such cell would allow development of such procedures. Identification of NRBCs in blood samples would be possible provided that specific antibodies are available. Here we have isolated recombinant antibodies using phage display. From the panel of antibody fragments specifically recognising ε-Hb, one was chosen for further characterization, DAb1. DAb1 binds to ε-Hb both in Western blots and immunocytochemistry. Several ε-Hb positive cells were detected in a blood sample taken as postchorionic villus sampling (CVS). To evaluate the sensitivity of the method, K562 cells (which express ε-Hb) were spiked in a blood sample followed by staining in solution and FACS analysis.

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Comparison of methods for erythroblast selection: Application to selecting fetal erythroblasts from maternal blood

Cited by 13 publications

References 40 publications

Blood-on-a-Chip

Blood-on-a-Chip

A novel method for depositing erythroid cells onto glass slides for fetal cell analysis

Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis

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