Comparison of Methods to Transform Embyrogenic Cotyledons of Melon

Gray, D. J.; Hiebert, E.; Lin, Chun-Yi; Kelley, Karen; Compton, Michael E.; Gaba, Victor

doi:10.21273/hortsci.30.4.788b

Cited by 7 publications

(6 citation statements)

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“…Comparing Agrobacteriunt. transformation and particle bombardement, Gonsalves et aL (1994) and Gray et al (1995) indicates that a similar percentage of transgenic shoots and embryos is obtained by both methods.…”

Section: Technique Of Transformationmentioning

confidence: 83%

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Melon Biotechnology

Guis

Roustan

Dogimont

et al. 1998

Biotechnology and Genetic Engineering Reviews

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Section: Technique Of Transformationmentioning

confidence: 83%

“…Particle gun transformation has been described by Gaba et aJ. (1992) and Gonsalves et al (1994) using organogenic explants and by Gray et al (1995) using embryogenic explants. Comparing Agrobacteriunt.…”

Section: Technique Of Transformationmentioning

confidence: 99%

Melon Biotechnology

Guis

Roustan

Dogimont

et al. 1998

Biotechnology and Genetic Engineering Reviews

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“…Melon transformation has been successfully obtained with reporter genes (Fang and Grumet 1990;Gray et al 1995;Akasaka-Kennedy et al 2004) and several plant traits, such as virus resistance (Fang and Grumet 1993;Yoshioka et al 1993;Gonsalvez et al 1994;Fuchs et al 1997;Huttner et al 2001;Yalcin-Mendi et al 2004;Gaba et al 2004 ;Wu et al 2010), salt tolerance (Bordas et al 1997) and long fruit shelf life (Ayub et al 1996;Ezura et al 1997;Silva et al 2004;Curuk et al 2005;Papadopoulou et al 2005;Nunez-Palenius et al 2006) have been incorporated into different melon types or into other Cucurbitaceae (Han et al 2005;Ntui et al 2010). Transformation capacity varies widely according to the cultivar Comment citer ce document : Chovelon, V., Restier, V., Giovinazzo, N., Dogimont, C., Aarrouf, J.…”

Section: Introductionmentioning

confidence: 99%

Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?

et al. 2011

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Melon (Cucumis melo L.) is widely considered as a recalcitrant species for genetic transformation. In this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. The highest regeneration rate (1.13 ± 0.02 plants per explant) was obtained using cotyledon explants of the 'Védrantais' genotype on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l dimethylallylaminopurine (2-iP). Agrobacterium tumefaciens-mediated transformations with the uidA reporter gene were realized on cotyledon explants cultivated in these conditions: 70-90% of explants expressed a transient GUS activity during the early stages of regeneration, however, only few transgenic plants were obtained (1.8-4.5% of stable transformation with the GV2260pBI101 strain). These results revealed a low capacity of melon GUS-positive cells to regenerate transgenic plants. To evaluate the influence of the Agrobacterium infection on plant regeneration, histological analyses were conducted on explants 2, 7, 15, and 28 days after co-culture with the GV2260pBI101 strain. Genetic transformation occurred in epidermal and sub-epidermal cells and reached the meristematic structures expressing a high level of GUS activity during 14 days of culture; but after this period, most of the meristematic structures showed premature cell vacuolization and disorganization. This disruption of the GUS-positive meristematic areas could be responsible of the difficulties encountered to regenerate melon plants after genetic transformation.

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“…Gaba et al [63] and Gonsalves et al [64] used particle gun bombardment to transform muskmelon explants and recover transgenic plants through organogenesis. Gray et al [65] used the same transformation protocol and recovered plants from embryogenic materials. Gonsalves et al [64] and Gray et al [65] reported both that A. tumefaciens and microprojectile gene transfer produced almost the same percentage of transgenic plants.…”

Section: Improvement Of Muskmelon Using Recombinant Technologymentioning

confidence: 99%

“…Gray et al [65] used the same transformation protocol and recovered plants from embryogenic materials. Gonsalves et al [64] and Gray et al [65] reported both that A. tumefaciens and microprojectile gene transfer produced almost the same percentage of transgenic plants. Transformation success via Agrobacterium or particle gun bombardement is genotype-, explant source-, and in vitro culture conditions-dependent [64][65][66][67][68][69][70][71][72][73].…”

Section: Improvement Of Muskmelon Using Recombinant Technologymentioning

confidence: 99%

Melon Crops Improvement through Biotechnological Techniques for the Changing Climatic Conditions of the 21st Century

Sultana¹,

Rahman²

2014

IJGG

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As fleshy fruits, two major melon genera are widely produced and consumed, including the watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] and muskmelon (Cucumis melo L.). As different cultivars, cultivated them according to consumer and grower's choice, and market conditions (i.e. seedless varieties, good shelf life, etc.). China is the world's largest producer of melons, accounted for over 52% production by weight. In the past, melons considered to be a 'seasonal delight' because of their limited availability throughout the year, however, for production flexibility, imports availability and popularity, melons are now available in year-round. In recent, the melon production is 20% within total fruits production worldwide. Over the next three decades, a forty percent increase in the production of melons is required for a growing population. For the decade to 2009, the production of total melons approximately doubled by 2005 and after which it stabled. The inherent complexities in achieving this unparalleled increase in the demands of melon fruits are worsen by the yield-depressing consequences of climate alterations or dissimilarities. With declining agricultural land and water resources, and assailing microbes and pests, the expected increases in the melons production cannot be reached at an efficient level. Elite varieties of melons such as, tolerance to drought condition and inferior lands, or disease resistant that can yield more with fewer inputs will be pivotal to success. Moreover, varieties of melons can improve with the traits such as, seedless, good shelf life, excellent flesh color and good shapes that will make them more attractive to consumers. Plant breeding methods (conventional or molecular breeding) have to be applied for regenerate the elite varieties in melon fruits to meet the global demand for next decades. This paper highlights some of the scientific and technological tools that ought to be the necessary for all improvement programs of the melon production.

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Comparison of Methods to Transform Embyrogenic Cotyledons of Melon

Cited by 7 publications

References 0 publications

Melon Biotechnology

Melon Biotechnology

Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?

Melon Crops Improvement through Biotechnological Techniques for the Changing Climatic Conditions of the 21st Century

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