Comparison of methods used for the determination of void volume in reversed-phase liquid chromatography

Krstulović, Ante M.; Colin, Henri; Guiochon, Georges

doi:10.1021/ac00251a009

Cited by 134 publications

(49 citation statements)

References 16 publications

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“…The Kee value for sorption of lindane by Michawye soil was taken from the work of Miller and Weber (1986), in which the isotherm was measured under conditions similar to those described in this paper. Corrected retention times (the difference between a solute and a relatively unretained internal standard retention time) are used in lieu of capacity factors because unretained tracers commonly utilized to determine the reverse phase column void volumes yield different retention times (Krstulovic et al, 1982;Chin et al, 1986). Acetone was selected as the internal standard to which all target compounds were referenced; it was found to be relatively unretained, innocuous to the system, and easily detected.…”

Section: Correlations Between Partition Coefficients and Hprplc Retenmentioning

confidence: 99%

Estimating soil/sediment partition coefficients for organic compounds by high performance reverse phase liquid chromatography

1988

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Al~raet--High performance reverse phase liquid chromatography (HPRPLC) retention parameters were correlated to orgauic-carbon-normalized partition coefficient (Ko~) values for sorption of various uncharged organic substances onto sediments and soils in an attempt to devise a model for predicting sorption behavior. The results reveal a relationship between the logarithm of HPRPLC retention time and log Ko¢ that appears to provide a reasonable means for estimating partitioning properties from HPRPLC data. The technique was compared to methods which utilize octanol/water partition coefficients (Ko~) and aqueous solubilities as surrogate predictors of sorption behavior and was found to yield superior correlations in all cases tested.

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Section: Correlations Between Partition Coefficients and Hprplc Retenmentioning

confidence: 99%

Estimating soil/sediment partition coefficients for organic compounds by high performance reverse phase liquid chromatography

1988

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“…Examples include using an unretained uncharged or charged compound, pycnometry, or the minor disturbance method [6,7]. The simplest and most popular, and maybe the less accurate, method is to use unretained markers, e.g., thiourea, sodium nitrate, or uracil.…”

Section: Holdup Volumementioning

confidence: 99%

Highlighting Important Parameters Often Neglected in Numerical Optimization of Preparative Chromatography

et al. 2011

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When a chromatographic separation process is numerically optimized, a number of input parameters to the column model need to be accurately determined, and the accuracy will affect the reliability of the predicted optimal operational conditions. Furthermore, the numerical accuracy of the solution to the column model will have similar impact. The input parameters holdup volume, injection profiles, and the selection of an algorithm for solving the column model were investigated. Errors in parameters or a numerical error in the solution of the column model were found to lead to a prediction of a product fraction where the set purity and/ or yield requirements are not met.

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“…---l + k n h , n tM (27) Perturbations are introduced to these (1 + k, ) values andtM calculations are performed repeatedly.…”

Section: Computer Simulation Approachmentioning

confidence: 99%

Evaluation of dead time calculation in liquid chromatography using a linearization procedure for homologous series

Tulder

Franke

Zeeuw

1987

J. High Resol. Chromatogr.

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SummaryA mathematical method for the calculation of the dead time (tM) in HPLC was evaluated using a computer simulationapproach, in which artificial perturbations were introduced to simulated homolog retention times. The calculation was based on a modified and extended Grobler and Balisz (GB) method. Investigated was how the precision of the calculated tM is affected by: statistical fluctuations in retention timesand which, and how many homolog retention times are used. Based on these simulations a two-step procedure for the tM calculation is proposed: In the first step the linearity of log tR,, vs carbon number n is checked using as many homolog retention times as possible. The slope value bo of the first linear regression in the GB method is used for the selection of homolog retention times in the final tM calculation. In the second step the optimal selection of homologs is made and the final ty calculation is carried out. Guidelines for homolog selection are given.

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Comparison of methods used for the determination of void volume in reversed-phase liquid chromatography

Cited by 134 publications

References 16 publications

Estimating soil/sediment partition coefficients for organic compounds by high performance reverse phase liquid chromatography

Estimating soil/sediment partition coefficients for organic compounds by high performance reverse phase liquid chromatography

Highlighting Important Parameters Often Neglected in Numerical Optimization of Preparative Chromatography

Evaluation of dead time calculation in liquid chromatography using a linearization procedure for homologous series

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