Comparison of microemulsion electrokinetic chromatography and micellar electrokinetic chromatography as methods for the analysis of ten benzophenones

Abstract

Microemulsion electrokinetic chromatography (MEEKC) and micellar electrokinetic chromatograpy (MEKC) were compared for their abilities to separate and detect ten similar benzophenones, which are commonly used as UV filters in various plastic and cosmetic products. Sodium dodecyl sulfate (SDS) concentration and column temperature rarely affected separation resolution for MEEKC, but separation of benzophenones could be improved by changing the SDS concentration and column temperature for MEKC. Buffer pH and ethanol (organic modifier) were found to markedly influence the separation selectivity for both MEEKC and MEKC systems. In addition, a higher electric voltage improved the separation efficiency without a noticeable reduction in separation resolution for MEEKC, whereas it caused a poor separation resolution for the MEKC system.

The microemulsion electrokinetic capillary chromatography combined with reversed‐electrode polarity stacking mode for enriching and quantifying lignanoids and ginsenosides in TCMs preparation Shengmai injection

“…Compared with micelle, microemulsions with a core of oil in micelle are more stable and cover wider range of solubility. Although the composition of MEEKC is more complex, separation efficiency of MEEKC is higher than MEKC especially in the analysis of samples with complex matrix and this result has been showed in previous reports . A MEEKC method was reported for separating and determining anthraquinones in Paedicalyx attopevensis Pierre ex Pitard , one of the TCMs .…”

Separation and on-line preconcentration by stacking and sweeping of charged analytes in the plant by microemulsion electrokinetic chromatography with nonionic surfactants

“…The separation mechanism in MEEKC is very similar to what is known from micellar electrokinetic chromatography (MEKC), with the main difference that the microemulsion has a core of tiny droplets of oil inside the micelles. Recently, a series of reports had concluded MEEKC has been shown to be applicable to a wider range of analytes and is able to provide higher separation efficiency than MEKC [16][17][18]. In MEEKC, a specific mixture of oil drops, surfactant, cosurfactant and aqueous buffer concentration along with an optimized condition in the capillary is adjusted to obtain and maintain a stable microemulsion phase when an on-line concentration method is applied.…”

Using inclusion complexes for achieving second‐order advantage: A novel technique for cinnamic acid derivatives analysis with second‐order calibration methods

“…Due to the prospective beneficial properties of these compounds regarding human health, extensive evidence about other characteristics such as anti‐carcinogenic and anti‐inflammatory properties, as well as cardiovascular protection, have been described . Many different analytical techniques have been used for the determination of phenolic acids, including gas chromatography‐mass spectrometry (GC‐MS), capillary electrophoresis (CE) methods, and micellar electrokinetic chromatography (MEKC) . In addition, the most widely used methods are based on reversed‐phase high‐performance liquid chromatography (RP‐HPLC) coupled with UV‐Vis detection and/or mass spectrometry .…”

“…[11][12][13][14] Many different analytical techniques have been used for the determination of phenolic acids, including gas chromatography-mass spectrometry (GC-MS), [15][16][17] capillary electrophoresis (CE) methods, [18][19][20] and micellar electrokinetic chromatography (MEKC). 21,22 In addition, the most widely used methods are based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with UV-Vis detection and/or mass spectrometry. [23][24][25] The reported methods not only require sophisticated analytical instrumentation, these are time-consuming, and also difficult to apply to the analysis of a complex matrix sample without any pretreatment.…”