Comparison of migration and invasiveness of epithelial tumor and melanoma cells in vitro

Wach, Franz; Eyrich, Anna-Maria; Wustrow, T. P. U.; Krieg, Thomas; Hein, Rüdiger

doi:10.1016/0923-1811(95)00470-x

Cited by 23 publications

(30 citation statements)

References 29 publications

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“…10) On the other hand, it has been demonstrated that tumor metastasis is affected by the microenvironment of the target organs 22) and that the metastatic properties of tumor cells vary according to different kinds of tumor cells. 23) Therefore, the anti-metastatic effect of evodiamine was further examined in terms of the liver metastasis of colon 26-L5 cells and the lung metastasis of LLC cells. As shown in Table 1, the exposure of colon 26-L5 cells to 30 mM evodiamine before inoculation caused significant suppression (pϽ0.01) of liver metastasis, which was almost the same inhibition rate as that in lung metastasis.…”

Section: Resultsmentioning

confidence: 99%

Anti-Invasive and Metastatic Activities of Evodiamine.

Ogasawara

Matsunaga

Takahashi

et al. 2002

Biological & Pharmaceutical Bulletin

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Tumor metastasis is a major cause of death in cancer patients, and its blockade has been considered to enable cancer patients to survive. 1) Thus, it is important to find promising agents with anti-metastatic activity.The cascade of cancer metastasis comprises a complex multistep process, and tumor invasion into the extracellular matrix plays an important role in tumor metastasis.2) Various products have been shown to possess anti-invasive activities, and some of them are demonstrated to be able to prevent tumor metastasis. [3][4][5][6][7][8][9] We have recently reported that evodiamine can markedly inhibit in vitro invasion and lung metastasis by colon 26-L5 cells. 10)Evodiamine is one of the main constituents of Evodiae Fructus 11) and has been shown to possess anti-tumor growth, 12) anti-aldosterone releasing, 13) anti-obesity, 14) bronchoconstrictive, 15) anti-nociceptive, 16) vasorelaxant, 17) catecholamine-secretory 18) and anti-nitric oxide producing 19) properties. On the other hand, the anti-invasive and metastatic effects of evodiamine have not been fully elucidated.To extend our study, we investigated here the anti-invasive and metastatic activities of evodiamine on lung carcinoma and melanoma cells, in addition to colon carcinoma cells, and estimated critical structures of evodiamine for the inhibition of tumor cell invasion, migration and metastasis using compounds having structures similar to that of evodiamine. Evodiamine,13,13b,3Ј : 3,4] pyrido [2,1-b] quinazolin-5 one, was purchased from Matsuura Yakugyo Co., Ltd. Rutaecarpine, yohimbine, reserpine and indole were purchased from Wako Pure Chem. Ind., Ltd. 2-Mercapto-4(3H)-quinazolinone was purchased from Tokyo Kasei Kogyo Co., Ltd. These compounds were dissolved in dimethylsulfoxide and used in this study. MATERIALS AND METHODS Materials Cells and Cell CultureMurine colon 26-L5 adenocarcinoma was kindly provided by Prof. I. Saiki (Toyama Med. Pharm. Univ., Inst. of Natural Medicine, Toyama, Japan). Murine B16-F10 melanoma was kindly provided by Dr. S. Wakuzawa (Hokuriku Univ., Kanazawa, Japan). Lewis lung carcinoma (LLC) was purchased from RIKEN Cell Bank. Colon 26-L5 cells and B16-F10 cells were maintained in RPMI-1640 supplemented with 10% fetal calf serum (FCS), 2-mercaptoethenol, 100 U/ml penicillin, and 0.1 mg/ml streptomycin. LLC was maintained in DMEM with 10% FCS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin.Animals Inbred 6 week-old female Balb/c mice were purchased from Shizuoka Laboratory Animal Center, Hamamatsu, Japan. All mice were housed in a controlled environment with a 12 h light/dark cycle, a temperature of 24Ϯ2°C and humidity at 55Ϯ10%, and they were given commercial food and tap water ad libitum. After an acclimatization period of 1 week, these mice were used in the present study.Cell Invasion and Migration Assays Tumor cell invasion through a reconstituted basement membrane (Matrigel) were assayed according to the methods reported previously. 20)Briefly, in transwell cell culture chambers, filters of 8 mm pore size were ...

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Section: Resultsmentioning

confidence: 99%

Anti-Invasive and Metastatic Activities of Evodiamine.

Ogasawara

Matsunaga

Takahashi

et al. 2002

Biological & Pharmaceutical Bulletin

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“…Invasion assays were performed in Boyden chambers containing polycarbonate filters with 8-m pore size (Costar, Bodenheim, Germany) essentially as described previously (Albini et al, 1987;Terranova et al, 1986;Wach et al, 1996). Filters were coated with a commercially available reconstituted basement membrane (Matrigel, diluted 1:3 in H 2 O; Becton Dickinson #356235, Heidelberg, Germany), fibronectin (1 g/ cm 2 , 12.5 nmol/filter; Becton Dickinson #40008), laminin-1 (5 g/cm 2 , 5 nmol/filter; Becton Dickinson #40232), tenascin (1 g/cm 2 , 1 nmol/filter; Becton Dickinson #40239), collagen type I (1 g/cm 2 , 6.5 nmol/filter; Becton Dickinson #40243), type II (1 g/ cm 2 , 6.5 nmol/filter; Becton Dickinson #40234), and type IV (1 g/cm 2 , 5 nmol/filter; Becton Dickinson #40233), vitronectin (50 ng/cm 2 , 1 nmol/filter; Becton Dickinson #40238), or HSPG (2 g/cm 2 , 1 nmol/filter; Becton Dickinson #40250).…”

Section: Invasion and Attachment Assaymentioning

confidence: 99%

Active Detachment Involves Inhibition of Cell-Matrix Contacts of Malignant Melanoma Cells by Secretion of Melanoma Inhibitory Activity

Bosserhoff

Stoll

Sleeman

et al. 2003

Laboratory Investigation

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SUMMARY:Melanoma inhibitory activity (MIA) has been identified as a small protein secreted from malignant melanoma cells.Recent results revealed a direct interaction of MIA and epitopes within extracellular matrix proteins including fibronectin. The aim of this study was to analyze functional consequences mediated by this interaction. Here we show that MIA interferes specifically with attachment of melanoma cells to fibronectin, a phenomenon we refer to as active detachment. Antibodies inhibiting binding of ␣4␤1 and ␣5␤1 integrins to fibronectin cross-react specifically with MIA, suggesting that MIA shares significant structural homology with the binding pockets of these integrins and thereby masks the respective epitopes on extracellular matrix molecules. Several peptides derived from fibronectin and from a phage display screening were tested with respect to a potential MIA-inhibitory effect. In vitro tests identified two peptides affecting MIA function; both inhibited growth of melanoma metastases in vivo. In summary, we conclude that MIA may play a role in tumor progression and spread of malignant melanomas via mediating active detachment of cells from extracellular matrix molecules within their local milieu. Further, our results suggest that inhibiting MIA functions in vivo may provide a novel therapeutic strategy for metastatic melanoma disease. (Lab Invest 2003, 83:1583-1594.

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“…The gene expression profiling of the highly invasive and metastatic BLM and MV3 melanoma cell lines, 9,11,12 the low/moderate invasive A2058 and SK-mel28 cell lines 13,20 and the nonmetastatic WM164 cell line 21,22 followed by hierarchal cluster analysis showed the highly invasive and metastatic cell lines BLM and MV3 cell lines cluster together. Whereas, the melanoma cells lines A2058, SK-mel28, which display moderate to low invasive phenotypes, and WM164, which is not invasive, clustered together (Fig.…”

Section: Gene Expression Profiles Of Melanoma Cell Linesmentioning

confidence: 99%

“…Interestingly, the gene expression profiles of the various melanoma cell lines mirrored the invasive/metastatic phenotype described for those cell lines. 9,[11][12][13][20][21][22] Ontological characterization of gene expression profiles of the invasive melanoma cell lines BLM and MV3 compared to noninvasive WM164 line…”

Section: Gene Expression Profiles Of Melanoma Cell Linesmentioning

confidence: 99%

“…1,7,8 A variety of melanoma cell lines have been characterized as having different potentials for invasion and metastasis in animal models of melanoma. [9][10][11][12][13][14] The molecular characteristics of some of these lines have been investigated with the aim of shedding light of critical components in the metastatic process. Using gene expression analysis, de Wit et al (2005) found a subset of genes that were differentially expressed in metastatic and nonmetastatic melanoma cell lines.…”

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confidence: 99%

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The invasive potential of human melanoma cell lines correlates with their ability to alter fibroblast gene expression in vitro and the stromal microenvironment in vivo

Dragulev

Zigrino

et al. 2009

Intl Journal of Cancer

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The tumor microenvironment is thought to play an important role in invasion and metastasis. Previously, we have shown that signaling from melanoma cells can alter the gene expression profiles of fibroblasts in vitro and in vivo. To investigate whether the capacity to signal fibroblasts and alter host gene expression profiles is correlated to the invasive potential of specific human melanoma cell lines, we assayed changes in gene expression of fibroblasts when cocultured with the human melanoma cell lines BLM, MV3, A2058, SK-mel28 and WM164. Results indicated that the gene expression of key chemokines and cytokines, such as IL-1B, IL-8, IL-6 and CCL2/MCP1, was significantly upregulated in fibroblasts cocultured with the invasive melanoma lines BLM and MV3 compared to fibroblasts cocultured with noninvasive WM164 cells. The results were verified by quantitative RT-PCR as well as by protein assay and supported by immunohistochemistry of human invasive melanoma. Furthermore, a role for fibroblast-secreted IL-1B in the invasion of melanoma was demonstrated in vitro, where siRNA silencing of IL-1B in melanoma-stimulated fibroblasts resulted in a diminution of melanoma invasion. Although CCL2/MCP1, a chemoattractant for macrophages, was shown to be upregulated in fibroblasts cocultured with metastatic melanoma cell lines, immunohistochemical analysis of human melanoma also indicated CCL2/MCP1 production associated with the melanoma. In summary, these experiments indicate that the invasiveness of melanoma can partly be correlated to its ability to stimulate host stromal fibroblasts to give rise to the secretion of chemokines that generate a microenvironment that is conductive for melanoma invasion and metastasis. ' UICCKey words: melanoma; stroma; metastasis; IL-1B; invasion; gene expression; microenvironmentThe capacity for invasion and metastasis is one of the hallmarks of tumor malignancy. The role of host-tumor communication and the tumor microenvironment in tumor cell invasion and metastasis has been recognized as being critical in this process. 1 The complex interaction between invasive tumor cells, stromal cells, lymphocytes and extracellular matrix as represented by the invasive tumor microenvironment is only beginning to be understood. Further investigation in tumor microenvironments will generate new possibilities in terms of identifying key mechanisms of tumor cell invasion and metastasis and potentially therapeutic nodes for attenuating primary tumor metastasis. 2,3 Fibroblasts comprise one of the principal cell types of the stromal compartment, and recent studies have demonstrated that stromal fibroblasts are important in tumor cell migration through the stroma.4,5 Stimulation of resident stromal fibroblasts by the presence of invasive tumor cells gives rise to alterations in the gene and protein expression patterns of these cells, which have been interpreted as proinvasive and prometastatic.6 Upregulation of key chemokine and cytokine expression in stromal fibroblasts in the presence of migrating human m...

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Comparison of migration and invasiveness of epithelial tumor and melanoma cells in vitro

Cited by 23 publications

References 29 publications

Anti-Invasive and Metastatic Activities of Evodiamine.

Anti-Invasive and Metastatic Activities of Evodiamine.

Active Detachment Involves Inhibition of Cell-Matrix Contacts of Malignant Melanoma Cells by Secretion of Melanoma Inhibitory Activity

The invasive potential of human melanoma cell lines correlates with their ability to alter fibroblast gene expression in vitro and the stromal microenvironment in vivo

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