Anti-Invasive and Metastatic Activities of Evodiamine.

Ogasawara, M.; Matsunaga, Takayuki; Takahashi, Satoshi; Saiki, Ikuo; Suzuki, Hideyo

doi:10.1248/bpb.25.1491

Cited by 84 publications

(57 citation statements)

References 22 publications

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“…Tumor invasion into the extracellular matrix plays an important role in tumor metastasis (18). In this study, B16-F10 cells induced by FN passed through polycarbonate membrane coated with Matrigel in a transwell cell-culture chamber, in which the number of invading cells reflected the invasive capacity of the cells.…”

Section: Inhibitory Effect Of Ysl On B16-f10 Invasionmentioning

confidence: 97%

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Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

Yao

Che

Lü

et al. 2007

Mol Med

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Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide, comprised of three amino acids, that has shown antitumor effects on human hepatocarcinoma BEL-7402 in vitro and in vivo. In this study, we evaluated the inhibition of YSL on invasion and adhesion of the mouse B16-F10 melanoma cell line by injecting B16-F10 cells into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model. YSL inhibited B16-F10 cell metastasis to lung, reducing the number and area of metastasis lesions. When we treated B16-F10 cells with YSL (0.01, 0.1, 1, 10, or 100 μg/mL) in vitro, we found that YSL inhibited the proliferation of B16-F10 cells with a 28.11% rate of inhibition. YSL significantly decreased the adhesiveness of B16-F10 cells to Matrigel with a 29.15% inhibition rate; YSL also significantly inhibited the invasion of B16-F10 cells, producing an inhibition of 35.31%. By analyses with Western blot and real-time RT-PCR, we found that YSL markedly inhibited the expression of ICAM-1 in B16-F10 cells. These data suggest that YSL inhibits the growth, invasion, and adhesion of B16-F10 cells.

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Section: Inhibitory Effect Of Ysl On B16-f10 Invasionmentioning

confidence: 97%

“…Tumor metastasis begins from invasion. Tumor invasion includes the penetration of blood vessels and lymphatic vessels, and the invasion of target organs by tumor cells and plays an important role in tumor metastasis (18). Liotta et al (23) proposed a three-step hypothesis describing the sequence of biochemical events during tumor cell invasion.…”

Section: A B C Dmentioning

confidence: 99%

Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

Yao

Che

Lü

et al. 2007

Mol Med

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“…Cell invasion and migration abilities were determined using a Modified Boyden Chamber assay as described previously (Ogasawara et al, 2002;Siripong, et al, 2002;Roy and Maity, 2007). For analyzing the invasive ability, transwell ® chambers (Costar 3422, Corning, NY, U.S.A.) were set up with 8 µm pore size of polyvinylpyrrolidonefree carbonate filters (Nucleopore, Pleasanton, USA).…”

Section: Transwell Invasion and Migration Assaysmentioning

confidence: 99%

Anti-metastatic Effects on B16F10 Melanoma Cells of Extracts and Two Prenylated Xanthones Isolated from Maclura amboinensis Bl. Roots

Siripong¹,

Rassamee²,

Piyaviriyakul³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Inhibitory effects of Maclura amboinenesis Bl, one plant used traditionally for the treatment of cancers, on metastatic potential of highly metastatic B16F10 melanoma cells were investigated in vitro. Cell proliferation was assessed using the MTT colorimetric assay. Details of metastatic capabilities including invasion, migration and adhesion of B16F10 melanoma cells were examined by Boyden Chamber invasion and migration, scratch motility and cell attachment assays, respectively. The results demonstrated that n-hexane and chloroform extracts exhibited potent anti-proliferative effects (p<0.01), whereas the methanol and aqueous extracts had less pronounced effects after 24 h exposure. Bioactivity-guided chromatographic fractionation of both active n-hexane and chloroform extracts led to the isolation of two main prenylated xanthones and characterization as macluraxanthone and gerontoxanthone-I, respectively, their structures being identified by comparison with the spectral data. Interestingly, both exhibited potent effective effects. At non-toxic effective doses, n-hexane and chloroform extracts (10 and 30 µg/ml) as well as macluraxanthone and gerontoxanthone-I (3 and 10 µM) significantly inhibited B16F10 cell invasion, to a greater extent than 10 µM doxorubicin, while reducing migration of cancer cells without cellular cytotoxicity. Moreover, exposure of B16F10 melanoma cells to high concentrations of chloroform (30 µg/ml) and geratoxanthone-I (20 µM) for 24 h resulted in delayed adhesion and retarded colonization. As insights into mechanisms of action, typical morphological changes of apoptotic cells e.g. membrane blebbing, chromatin condensation, nuclear fragmentation, apoptotic bodies and loss of adhesion as well as cell cycle arrest in the G1 phase with increase of sub-G1 cell proportions, detected by Hoechst 33342 staining and flow cytometry were observed, suggesting DNA damage and subsequent apoptotic cell death. Taken together, our findings indicate for the first time that active n-hexane and chloroform extracts as well as macluraxanthone and gerontoxanthone-I isolated from Maclura amboinensis Bl. roots affect multistep of cancer metastasis processes including proliferation, adhesion, invasion and migration, possibly through induction of apoptosis of highly metastatic B16F10 melanoma cells. Based on these data, M. amboinensis Bl. represents a potential candidate novel chemopreventive and/or chemotherapeutic agent. Additionally, they also support its ethno-medicinal usage for cancer prevention and/or chemotherapy.

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“…It is well known for its anti-tumor, anti-inflammatory, and weight reduction effects. Moreover, it has been used in the treatment of abdominal pain, diarrhea, nausea, and for many other pharmacological effects (Ogasawara et al, 2002;Shyu et al, 2006;Ko et al, 2007;Wang et al, 2008). In addition, evodiamine can induce the lipolysis rate in mice and enhance specific binding of guanosine diphosphate to mitochondria in interscapular brown adipose tissue, where it increases heat dissipation and promotes energy consumption.…”

Section: Introductionmentioning

confidence: 99%

Long-term effects of evodiamine on expressions of lipogenesis and lipolysis genes in mouse adipose and liver tissues

Jiang¹,

Li²,

Yang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Evodiamine, the major alkaloid component isolated from the fruit of dried, unripened Evodia rutaecarpa Bentham, affects the plasma levels of cholecystokinin and various biological events such as gastric emptying and gastrointestinal transit; these effects of evodiamine were previously investigated in male rats. In this study, we aimed to investigate the effects of evodiamine on average daily weight gain, rectal temperature, and expressions of genes involved in lipid metabolism in liver and adipose tissues. Evodiamine was added as a supplement, comprising 0.02, 0.04, and 0.06% of the diet fed to mice for 1, 2, 3, and 4 weeks. Results showed that average daily weight gain and rectal temperature decreased significantly over time in a dosedependent manner. Evodiamine changed expressions of the peroxisome proliferator-activated receptor-g (PPARg) in mouse adipose and liver tissues in time-and dose-dependent manners. We found that evodiamine Long-term effects of evodiamine on lipid metabolism decreased mRNA expression of the sterol-regulatory element binding protein (SREBP-1c) and fatty acid synthase in adipose tissue. In addition, evodiamine increased expressions of hormone-sensitive lipase in both liver and adipose tissues. Interestingly, evodiamine increased the expression of triglyceride hydrolase only in adipose tissue. In conclusion, evodiamine could influence lipid metabolism through regulation of the expressions of its key genes, as well as reduce body heat and body weight.

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Anti-Invasive and Metastatic Activities of Evodiamine.

Cited by 84 publications

References 22 publications

Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

Anti-metastatic Effects on B16F10 Melanoma Cells of Extracts and Two Prenylated Xanthones Isolated from Maclura amboinensis Bl. Roots

Long-term effects of evodiamine on expressions of lipogenesis and lipolysis genes in mouse adipose and liver tissues

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