Long-term effects of evodiamine on expressions of lipogenesis and lipolysis genes in mouse adipose and liver tissues

Abstract

ABSTRACT. Evodiamine, the major alkaloid component isolated from the fruit of dried, unripened Evodia rutaecarpa Bentham, affects the plasma levels of cholecystokinin and various biological events such as gastric emptying and gastrointestinal transit; these effects of evodiamine were previously investigated in male rats. In this study, we aimed to investigate the effects of evodiamine on average daily weight gain, rectal temperature, and expressions of genes involved in lipid metabolism in liver and adipose tissues. Evodiamine was added as a supplement, comprising 0.02, 0.04, and 0.06% of the diet fed to mice for 1, 2, 3, and 4 weeks. Results showed that average daily weight gain and rectal temperature decreased significantly over time in a dosedependent manner. Evodiamine changed expressions of the peroxisome proliferator-activated receptor-g (PPARg) in mouse adipose and liver tissues in time-and dose-dependent manners. We found that evodiamine Long-term effects of evodiamine on lipid metabolism decreased mRNA expression of the sterol-regulatory element binding protein (SREBP-1c) and fatty acid synthase in adipose tissue. In addition, evodiamine increased expressions of hormone-sensitive lipase in both liver and adipose tissues. Interestingly, evodiamine increased the expression of triglyceride hydrolase only in adipose tissue. In conclusion, evodiamine could influence lipid metabolism through regulation of the expressions of its key genes, as well as reduce body heat and body weight.

Combination of berberine and evodiamine inhibits intestinal cholesterol absorption in high fat diet induced hyperlipidemic rats

“…Evodiamine is a naturally indoloquinazoline alkaloid isolated from Evodia rutaecarpa , attracting close attentions with its anti-cancer, anti-inflammation, and anti-obesity effects [ 13 – 17 ]. Many researches have indicated that both of berberine and evodiamine exerted on regulation of lipid metabolism [ 18 , 25 ], but whether they have a synergistic effect on preventing hyperlipidemia when combined together remains completely unclear.…”

“…Berberine was conformed to possess obvious cholesterol-lowering effect on serum TC, LDL-C, or non HDL cholesterol (non HDL-C) in plenty of researches [ 12 , 27 – 29 ]. Evodiamine was reported to have effect on lipid metabolism by regulation the key genes in fat synthesis [ 18 ]. The reductions of liver cholesterol might be a result of decreased cholesterol input arise from the inhibition of intestinal cholesterol absorption [ 30 ].…”

“…Kong et al [ 31 ] has been reported that 1000 mg/day berberine for three months could significantly lower total cholesterol and LDL-cholesterol by 29% and 25%. Another study has showed that evodiamine as a supplement comprising 0.02, 0.04, and 0.06% of the diet fed to mice for 4 weeks could influence lipid metabolism in time and dose-dependent manners [ 18 ]. However, minimal information on the adverse drug reactions of berberine and evodiamine supplementation is available.…”

“…Evodiamine, the major bioactive component found in Evodia rutaecarpa , possess beneficial pharmacological effects involving anti-cancer, anti-inflammation, anti-obesity and anti-diabetes effects [ 13 – 17 ]. Previous research has reported that evodiamine could influence lipid metabolism through regulation of the expressions of peroxisome proliferator-activated receptor-g (PPARg) in mouse adipose and liver tissues [ 18 ]. Furthermore, Chao DC et al found that co-administration of berberine and evodiamine could suppress tumor promotion primarily through AP-1 and/or NF-κB pathways in HepG2 cells [ 19 ].…”

Folate Functionalized and Evodiamine‐Loaded Pluronic Nanomicelles for Augmented Cervical Cancer Cell Killing

“…Evodiamine (Evo), an indolequinone alkaloid that occurs naturally, is one of the primary bioactive components of medicinal plants including plant extracts of Evodia rutaecarpa, Evodiae fructus , and Euonymus europaeus . [ 1 ] Evo has gained increasing attention for its potentially beneficial pharmacological applications including anti‐tumor, [ 2 ] anti‐inflammatory, [ 3 ] anti‐bacterial, [ 4 ] anti‐neurodegenerative, [ 5 ] anti‐obesity, [ 6 ] anti‐depressant, [ 7 ] and cardiac protective. [ 8 ] Reported evidence suggested that Evo has anticancer properties in vivo and in vitro by inducing apoptosis, inhibiting proliferation, invasion, and migration of various cancer cell lines including cervical cancer cell line (i.e., HeLa cells).…”