Comparison of Milk Ring Test; Serum Plate Agglutination Test and Polymerase Chain Reaction for the Detection of Bovine Brucellosis

Saleha, Shamim

doi:10.14737/journal.rjvp/2014/2.1.5.8

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…The difference in percentage of Brucella antibodies between cows 9.2% and buffaloes 7.5% groups is not significant and mostly owing to difference in sample size between cows and buffaloes groups. The result of overall occurrence is in agreement with previous studies from Yemen (20), Pakistan (24), and Kenya (25) where prevalence of Brucella antibodies ranged from 7% to 9.7% detected by MRT assay for raw milk samples from cattle and buffaloes. Indeed, lower rates of prevalence of Brucella antibodies were also reported from different countries.…”

Section: Discussionsupporting

confidence: 91%

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

Almashhadany¹

2019

IJVS

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This study was undertaken to monitor Brucella antibodies in the milk of cows and buffaloes in Erbil Governorate, Kurdistan Region, Iraq, using mik ring test (MRT) assay. A total of 210 samples of milk (130 from cows and 80 from buffaloes) were randomly collected from lactating females. The overall prevalence of Brucella antibodies in all the milk samples was 8.6% (18/210). The highest rate was 9.2% found in the cow milk (12/130), while the lowest rate was 7.5% of the buffalo's milk (6/80). Out of 210 milk samples, only 15 (7.1%) were culture-positive for Brucella; about 7.7% (10/130) and 6.3% (5/80) from cows and buffaloes respectively. In terms of comparison between MRT and standard milk culture method, MRT was found more sensitive (83%), specific (98%), with the accuracy of 97% in comparison to the employed culture approach to detect Brucellosis agents in milk. The results also revealed that 70% and 60% of isolates were Brucella abortus, while 30% and 40% were Brucella melitensis from the milk of cow and buffaloes respectively. The highest rate of frequency for Brucella antibodies according to MRT was found in February (12.1%), while the lowest rate was found in June (5.7%). This study emphasizes that Brucellosis is still a significant public health hazard in the Kurdistan region. The study recommends MRT adoption in routine monitoring of brucellosis in milk collection centre, dairy factories, and farm. Consumers are also recommended to sufficiently heat the milk to destroy this foodborne pathogen before consumption or industrial processing.

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Section: Discussionsupporting

confidence: 91%

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

Almashhadany¹

2019

IJVS

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“…These findings are in agreement of Saleha et al, (2014), they also revealed in their study that SPAT test showed more positive results compared to MRT. This might be due to fact that milk proteins hinder the brucella antibodies isolation (Akhtar et al, 2010).…”

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confidence: 90%

Sero-Prevalence of Brucellosis in Cattle in Southern Area of Khyber Pakhtunkhwa, Pakistan

2014

Res. j. vet. pract.

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Brucellosis considered as the most important zoonotic disease worldwide. The diagnosis of brucellosis in bovines requires the use of more than one serological test for routine diagnosis. Present study was conducted to estimate the occurrence and prevalence of brucellosis in the lactating dairy cattle located in Bannu and Lakki Marwat districts of Khyber PakhtunKhwa, Pakistan. A total of 302 blood and milk samples were collected from different breed of cattle. The blood and milk samples were screened for brucellosis on the basis of serum plate agglutination test (SPAT) and Milk Ring Test (MRT), respectively. The overall prevalence of brucellosis was found 5.2% and 10.9% in District Bannu and Lakki Marwat, respectively, moreover prevalence of brucellosis on basis of SPAT and MRT showed 7.94% and 3.97% respectively. On the basis of breeding pattern, prevalence of brucellosis was found 10.2%, 19% and 13% in local breed, Friesian cross and Jersey cross respectively and no significant association was found among them (P > 0.05). Higher prevalence of brucellosis was found in cattle with age group of greater than five years with statistically significant association (P<0.05). Moreover, in case of breeding practice the prevalence of brucellosis was found higher within unknown source of breeding followed by community bull and artificial insemination (P > 0.05). Furthermore, higher prevalence of brucellosis was found in animals that were set free for grazing compared to stall feeder animals (P > 0.05). It can be therefore concluded that regular screening of animals for brucellosis in these areas is required to control this disease.All copyrights reserved to Nexus® academic publishers

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“…However, this requires skilled personnel in a standard biosecurity laboratory (Nielsen and Yu, 2010). Serological tests are moderately easy to perform and provide a practical advantage in detecting the prevalence of disease in large animal populations and can be used for early detection of the status of animals and they seem to be less costly (Saleha et al, 2014). However, it appears that polymerase chain reaction (PCR) and other new molecular techniques are likely to be used as routine typing and fingerprinting methods for diagnosis of brucellosis in livestock and wildlife (Godfroid et al, 2010).…”

Section: Diagnosis Of Brucellosismentioning

confidence: 99%

A Review on Epidemiology and Public Health Importance of Brucellosis with Special Reference to Sudd Wetland Region South Sudan

2016

International Journal of Research Studies in Biosciences

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Brucellosis is one of the most important zoonotic diseases of public health implications causing socio-economic impacts on the livelihoods of the poor rural pastoralist communities and the urban population worldwide. Bovine brucellosis is one of the top five priority diseases of livestock in South Sudan. This review casts light on some epidemiological parameters and public health importance of brucellosis to enable key stakeholders to understand the magnitude of the disease in South Sudan. Of which over 85% of the population is associated with cattle directly or indirectly for improving their livelihoods and enhancing food and nutrition security. Rural people commonly contract the disease through drinking of raw milk or ingestion of improperly cooked meat from infected cattle or even aborted feti and stillbirths. Cattle are infected through ingestion of contaminated feed or water in the endemic areas. In the Sudd Wetland region of Terekeka County and Jonglei State brucellosis poses threats to socioeconomic development. Biotyping of Brucella species and One Health approach are needed to mitigate prevalence of the disease among the livestock and the rural farming communities in South Sudan.

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Comparison of Milk Ring Test; Serum Plate Agglutination Test and Polymerase Chain Reaction for the Detection of Bovine Brucellosis

Cited by 3 publications

References 8 publications

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Iraq

Sero-Prevalence of Brucellosis in Cattle in Southern Area of Khyber Pakhtunkhwa, Pakistan

A Review on Epidemiology and Public Health Importance of Brucellosis with Special Reference to Sudd Wetland Region South Sudan

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