Comparison of molecular and emulsifying properties of gum arabic and mesquite gum using asymmetrical flow field-flow fractionation

New studies on basil (Ocimum bacilicum L.) seed gum: Part I – Fractionation, physicochemical and surface activity characterization

“…Guo et al (2011) indicated that Artemisia sphaerocephala Krasch polysaccharide (ASKP) has two components with different molecular weights which lower molecular weight fraction exhibited higher surface activity nevertheless it contains no protein (Guo et al, 2011). The flexible chain structure also generates surface activity, which can increase adsorption at the interface and consequently reduces surface tension (Alftr en, Peñarrieta, Bergenståhl, & Nilsson, 2012). According to k H values shown in Table 3, SUPER-BSG exhibited highest flexibility.…”

Separation and characterization of food macromolecules using field-flow fractionation: A review

“…gums that are exudates from various trees and shrubs. Some gums have been characterized with FFF: xanthan gum (Pauck & Colfen, 1998;Viebke & Williams, 2000a), konjac glucomannans (Benincasa et al, 2002), gum Arabic (Alftrén, Peñarrieta, Bergenståhl, & Nilsson, 2012;Picton, Bataille, & Muller, 2000), mesquite gum (Alftrén et al, 2012) and pectin (Thielking & Kulicke, 1998).…”

“…The late eluting population (in the FlFFF analysis) had a non-Gaussian appearance which the authors remedied by changing to a linearly decaying cross flow rather than a stepwise decrease, illustrating the benefits of using continuously decaying programmed cross flows. By utilizing AF4 with fluorescence detection on fluorescently labeled gum arabic and mesquite gum the distribution of proteinaceous moieties over the size distribution was investigated (Alftrén et al, 2012). On the contrary to gum arabic, mesquite gum was found to contain one population with an evenly distributed content of proteinaceous matter.…”

Fluorescence-labelling for analysis of protein in starch using asymmetrical flow field-flow fractionation (AF4)

“…The labeling solution was prepared by mixing 10 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) and 10 mM 7-methoxycoumarin-3-carboxylic acid in the volume ratio of 1:1 for 3 h. The labeling solution was added to the starch solution in the volume ratio of 1:1 and mixed for 1 h, and then the solution was injected into the AF4 channel. 30 For detection of labeled components, the wavelength of the fluorescence detector was set at 336 nm for excitation and 402 nm for emission, respectively.…”