Comparison of Molecular Characteristics of Mycoplasma pneumoniae Specimens Collected from the United States and China

Yan, Chao; Sun, Hongmei; Lee, Stella; Selvarangan, Rangaraj; Qin, Xuan; Tang, Yi‐Wei; Waites, Ken B.; Zheng, Xiaotian

doi:10.1128/jcm.02468-15

Cited by 10 publications

(10 citation statements)

References 15 publications

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“…Previous studies also indicated a type‐shift trend between P1‐1 and P1‐2 and M4‐5‐7‐2 and M3‐5‐6‐2 in terms of the most prevalent type by year . We also found a close association between MLVA type M4‐5‐7‐2 and macrolide resistance in our previous study …”

Section: Introductionsupporting

confidence: 86%

Molecular and clinical characteristics of severe Mycoplasma pneumoniae pneumonia in children

Yan

Xue

Zhao

et al. 2019

Pediatric Pulmonology

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Objectives: To analyze the molecular and the clinical characteristics of Mycoplasma pneumoniae (Mp) pneumonia (MPP) and to explore the related factors predicting severe MPP (SMPP).Methods: A total of 423 pediatric cases of MPP were retrospectively analyzed, in 2013-2017, in Beijing, China. Clinical information was collected from the medical records. Mp-positive specimens were characterized using P1 typing and multiple locus variable-number tandem repeat analysis (MLVA). The macrolide resistanceassociated mutations were also detected.Results: The predominant genotype was P1-1 (88.2%) and M4-5-7-2 (87.5%), whereas percentages of type P1-2 and M3-5-6-2 increased across the 5-year period. The mutation rate of genotype M4-5-7-2 (365/370, 98.6%) was significantly higher than that of the genotype M3-5-6-2 (15/48, 32.25%; P = 0.000). Overall, 180 (42.6%) of the 423 Mp-positive patients were coinfected with other pathogens. Respiratory syncytial virus coinfection (24/180, 13.3%) was more common in cases typed M3-5-6-2 (4/23, 17.4%) than that of M4-5-7-2 (20/155, 12.9%; P = 0.038). Pleural effusion accounted for 52.6% (169/321) of the observed complications. In the mono-infection cases, cases typed M3-5-6-2 (56%, 14/25) were significantly (P = 0.020) associated with pleural effusion compared with those typed M4-5-7-2 (32.6%, 70/215); 84% (21/ 25) of specimens typed M3-5-6-2 were diagnosed as SMPP, whereas 63.7% (137/ 215) of specimens typed M4-5-7-2 were diagnosed as SMPP (P = 0.043). Conclusions:In our study, we proposed for the first time that the mono-infection patients with Mp typed M3-5-6-2 appear to have a higher risk for progressing to SMPP. MLVA typing can provide hints on the clinical characteristics of Mpp.

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Section: Introductionsupporting

confidence: 86%

Molecular and clinical characteristics of severe Mycoplasma pneumoniae pneumonia in children

Yan

Xue

Zhao

et al. 2019

Pediatric Pulmonology

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“…A limited amount of MRMP isolates and sporadic occurrence of other genotypes could be responsible for this phenomenon, whereas opposite results were found in areas with a higher incidence of MRMP. A correlation between increasing macrolide resistance and MLVA type 4/5/7/2 had been found in Hong Kong based on four years of data during 2011-2014 (Ho et al, 2015), as well as in the specimens collected from the United States and China (Sun et al, 2017;Yan et al, 2015). In the current study, similarly, all M. pneumoniae strains with P1 type 1 or MLVA type 4/ 5/7/2 were resistant to macrolide, however, those with type 2 lineages or type 3/5/6/2 could be probably sensitive to macrolide.…”

Section: Characteristicmentioning

confidence: 94%

“…Due to the significant regional differences in macrolideresistant (MR) rate, period, patient age and disease type among the studies (Pereyre et al, 2007;Cardinale et al, 2013;Diaz et al, 2015b;Ho et al, 2015;Kogoj et al, 2015), to date there are limited investigations on the distribution of M. pneumoniae types between adult and pediatric populations. Additionally, based on the finding from different regions (Diaz et al, 2015a,b;Ho et al, 2015;Kogoj et al, 2015;Sun et al, 2017;Yan et al, 2015), the correlation between macrolide resistance and M. pneumoniae genotype is still controversial.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and analysis of Mycoplasma pneumoniae among patients of all ages with community-acquired pneumonia during an epidemic in China

Chen

Bao

et al. 2019

International Journal of Infectious Diseases

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A B S T R A C TBackground: Analysis of the molecular characteristics of isolates is very important for clinical and epidemiological study of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae. Methods: Between 2010 and 2012, an epidemic period, M. pneumoniae was isolated from oropharyngeal swabs of consecutive CAP patients. Minimum inhibitory concentrations of macrolides, 23S rRNA gene sequencing, P1 gene and multilocus variable-number tandem-repeat analysis (MLVA) genotyping was conducted. Results: 88.3% (181/205) of the isolates were macrolide-resistant M. pneumoniae (MRMP) and all harbored an A2063 G mutation. The strains were clustered into 7 MLVA types, and P1 type 1 and type 2 lineages were co-circulated (86.3% and 13.7%). Compared with adults, no specific MLVA type contributed to higher M. pneumoniae infection in children (p = 0.14). Similar macrolide profile and genotypes of M. pneumoniae was found between outpatients and inpatients. Significant differences in proportion of P1 types and two main MLVA types 4/5/7/2 and 3/5/6/2 were observed between MRMP and macrolide-sensitive M. pneumoniae (MSMP) (p < 0.001).Conclusions: This study demonstrates a comprehensive profile of M. pneumoniae molecular characterization among CAP patients of all age, and provides more evidences on a correlation between MLVA type 4/5/7/2 and macrolide resistance in the setting of high incidence of MRMP.

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“…pneumoniae specimens directly from 480 polymerase chain reaction (PCR)-positive respiratory specimens collected at the Affiliated Children’s Hospital of the Capital Institute of Pediatrics, Beijing, China, between 2003 and 2015. Specimens collected from 2008–2013 (69.0%, 331/480) have been previously reported [6, 7, 9, 10], while specimens collected from 2003–2007(4.8%, 23/480) and 2014–2015 (26.2.%, 126/480) are being described for the first time. M .…”

Section: Methodsmentioning

confidence: 99%

“…pneumoniae genotypes, along with drug resistance profiles, can differ between regions and countries [6, 7]. , and even within the same region, type-shifts in predominant P1 genotypes have been recorded in Japan at intervals of about 10 years [8].…”

Section: Introductionmentioning

confidence: 99%

Changes in Molecular Characteristics of Mycoplasma pneumoniae in Clinical Specimens from Children in Beijing between 2003 and 2015

et al. 2017

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The molecular characteristics of 480 Mycoplasma pneumoniae polymerase chain reaction-positive specimens (331 were previously reported and 149 were newly reported) collected from pediatric patients in Beijing, China, between 2003 and 2015 were analyzed. Genotype M4-5-7-2/P1 were the most prevalent across the 13-year study period, although the isolation and mutation rates for this genotype varied between the periods 2003–2007, 2008–2013, and 2014–2015. In addition, there was a close association between the M4-5-7-2 genotype and macrolide resistance.

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Comparison of Molecular Characteristics of Mycoplasma pneumoniae Specimens Collected from the United States and China

Cited by 10 publications

References 15 publications

Molecular and clinical characteristics of severe Mycoplasma pneumoniae pneumonia in children

Molecular and clinical characteristics of severe Mycoplasma pneumoniae pneumonia in children

Molecular characterization and analysis of Mycoplasma pneumoniae among patients of all ages with community-acquired pneumonia during an epidemic in China

Changes in Molecular Characteristics of Mycoplasma pneumoniae in Clinical Specimens from Children in Beijing between 2003 and 2015

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