Comparison of mucosal phosphatase activity, phytate degradation, and nutrient digestibility in 3-week-old turkeys and broilers at different dietary levels of phosphorus and phytase

Mucosal phosphatase activity, phytate degradation, and mineral digestibility in 6-week-old turkeys and broilers at different dietary levels of phosphorus and phytase and comparison with 3-week-old animals

“…In this study, jejunal mucosal phosphatase activity, prececal InsP 6 disappearance, and prececal P and Ca digestibility were higher in 6-wk-old turkeys than in broilers without phytase supplementation. This is in contrast to the results obtained with 3-wk-old turkeys and broilers, where no differences between species were found in these traits in diets without phytase (Novotny et al., 2023) . This indicated that InsP 6 degradation in broilers and turkeys is affected differently by bird age.…”

“…In treatments with phytase supplementation, prececal InsP 6 disappearance was higher in broilers than in turkeys. This was also observed in 3-wk-old broilers and turkeys (Novotny et al., 2023) . However, differences in prececal InsP 6 disappearance between 6-wk-old turkeys and broilers were smaller than between 3-wk-old turkeys and broilers, as in broilers prececal InsP 6 disappearance remained at a high level of approximately 80% in CaP–PHY+; while in turkeys, prececal InsP 6 disappearance increased from 58.0% in wk 3 to 70.3% in wk 6 in CaP–PHY+ ( Figure 1 ).…”

“…HOH 59/19 TE) and was carried out at the experimental station “Unterer Lindenhof” of the University of Hohenheim, Germany. This was an extension of the trial described by Novotny et al. (2023) .…”

“…Mucosal phosphatase activity was measured in enriched brush-border membrane samples according to the methods of Huber et al. (2015) , with modifications described in the companion paper (Novotny et al., 2023) . In brief, brush-border membrane sample aliquots containing 160 µg protein were incubated with 25 µg of sodium phytate (Sirius Fine Chemicals SiChem GmbH, Bremen, Germany) at pH 5.5 for 15 min.…”

“…As two species with different maturation rates were compared ( Zuidhof et al., 2014 ; Tůmová et al., 2020 ), it was unclear whether the observed differences could be solely attributed to species effects or if physiological development effects were confounding. Therefore, the results were compared with those obtained from 3-wk-old broilers and turkeys of a companion study ( Novotny et al., 2023 ) to assess whether age affected the outcome of this species comparison. Another objective was to review the traits that might explain different endogenous InsP 6 degradation in broilers and turkeys, such as gut length, pH of the digestive tract, jejunal mucosal phosphatase activity, and changes between the third and sixth wk of age.…”