Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

Dal, Tuba; Kara, Soner Sertan; Çıkman, Aytekin; Balkan, Çiğdem Eda; Açıkgöz, Ziya Cibali; Zeybek, Hasan; Uslu, Hakan; Durmaz, Rıza

doi:10.1016/j.jiph.2018.11.008

Cited by 31 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the rapid development of molecular biology technology since the 1990s, molecular biological detection methods have been used to rapidly detect brucellosis at home and abroad [35][36][37]. For example, studies have shown that multiple real-time polymerase chain reactions can diagnose patients with negative serological tests, which makes up for the shortcomings of traditional serological tests and is considered an important detection method [38].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Characteristics and Spatiotemporal Trend Analysis of Human Brucellosis in China, 1950–2018

Yang

Zhang

Wang

et al. 2020

IJERPH

View full text Add to dashboard Cite

The rate of brucellosis, a zoonotic disease, has rapidly increased in humans brucellosis(HB) in recent years. In 1950–2018, a total of 684,380 HB cases (median 2274/year (interquartile range (IQR) 966–8325)) were reported to the National Infectious Disease Surveillance System in mainland China. The incidence of HB peaked in 2014 (4.32/100,000), and then showed a downward trend; we predict that it will maintain a steady downward trend in 2019–2020. Since 2015, the incidence of HB has shown opposite trends in the north and south of China; rates in the north have fallen and rates in the south have increased. In 2004–2018, the most significant increases in incidence of HB were in Yunnan (IQR 0.002–0.463/100,000), Hubei (IQR 0.000–0.338/100,000), and Guangdong (IQR 0.015–0.350/100,000). The areas where HB occurs have little overlap with areas with high per capita GDP in China. The “high–high” clusters of HB are located in northeastern China (Inner Mongolia, Heilongjiang, Jilin, Liaoning, Ningxia, Shanxi, and Gansu), and the “low–low” clusters of HB are located in southern China (Yunnan, Jiangxi, Shanghai, Guangxi, Guangdong, Zhejiang, Guizhou, and Hunan). In recent years, the incidence of HB in China has been controlled to some extent, but the incidence of HB has increased in southern China, and the disease has spread geographically in China from north to south. Further research is needed to address this change and to continue to explore the relationship between the incidence of HB and relevant factors.

show abstract

Section: Discussionmentioning

confidence: 99%

Epidemiological Characteristics and Spatiotemporal Trend Analysis of Human Brucellosis in China, 1950–2018

Yang

Zhang

Wang

et al. 2020

IJERPH

View full text Add to dashboard Cite

show abstract

“…Real-time PCR provides more specific and higher sensitivity diagnostic solutions than conventional PCR. Notwithstanding, diagnostic tests including serology and PCR may have their own limitations depending upon the time of sampling and the onset of infection in the animal/patient [22,23].…”

Section: Discussionmentioning

confidence: 99%

Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan

et al. 2020

View full text Add to dashboard Cite

Brucellosis, globally known bacterial zoonosis, is endemic to Pakistan. B. abortus in bovines, B. melitensis in small ruminants and B. canis in dogs mainly cause this disease. A total of 1821 sera (1196 from sheep and 625 from goats) from animal herds near the Pakistan–Afghanistan border were collected. In parallel testing of sera for anti-Brucella antibodies (B. abortus and B. melitensis) was carried out by RBPT and indirect ELISA. The presence of Brucella DNA in sera was tested by real-time PCR. The overall percentage of seropositive samples was 0.99 (18/1821) by both tests. All positive samples originated from Baluchistan territory which translated into 1.76% (18/1021). None of the positive sera had signals for Brucella DNA and none of sera from goats carried detectable antibodies. Both tests showed an almost perfect agreement with Kappa statistics. The flock size was found to be associated with the presence of anti-Brucella antibodies. The samples of Khyber Pakhtunkhwa (KPK) tested negative in both serological tests and hence were not processed for real-time PCR. The present study shows the presence of anti-Brucella antibodies in sheep in the Baluchistan region of Pakistan. Diagnostic services need to be improved and test and slaughter policies might be implemented for eradication of Brucella infection in these areas. Awareness about the infection is needed at the farmer’s level. Isolation and molecular biology of the isolates could help with understanding the prevailing etiology in a better way.

show abstract

“…The definitive diagnosis of brucellosis is made by isolating the microorganism from the blood or various tissue samples (such as the bone marrow, synovial fluid, etc.) or by identifying the causative agent based on the results of molecular tests like polymerase chain reaction (PCR) [10]. Besides the most commonly used diagnostic clinical laboratory test methods including Rose Bengal plate agglutination (RBPT), standard tube agglutination test (SAT), coombs antiglobulin test, and enzyme-linked immunosorbent assay; blood culture is recognized as the gold standard test [11][12].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Sensitivity and Specificity of Laboratory Tests Used in Differential Diagnosis of Childhood Brucellosis

Kazanasmaz¹,

Geter

2020

Cureus

View full text Add to dashboard Cite

Childhood brucellosis is a common public health problem in developing countries. The diagnosis of brucellosis based on nonspecific symptoms is an ongoing problem for physicians, especially in endemic areas. In this study, it is aimed to discuss the efficacy of frequently used test methods in the differential diagnosis of brucellosis. Methods The records of 332 patients admitted to pediatric clinic on suspicion of brucellosis were retrospectively analyzed. Patients with brucellosis were included in the positive group (n = 262) and those without brucellosis were included in the negative group (n = 70). Results As a result of biochemical analysis of the cases, median alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), erythrocyte sedimentation rate (ESR), and C reactive protein (CRP) values were significantly higher in the positive group than that in the negative group (p<0.05). There was no significant difference between median white blood cell, neutrophil, lymphocyte, neutrophil to lymphocyte ratio, hemoglobin, and platelet values between groups (p>0.05). Receiver operating curves were plotted to compare predictive values of CRP (area under curve (AUC): 0.635, p= 0.001), ESR(AUC:0.701, p<0.001), AST(AUC: 0.595, p=0.015), ALT(AUC:0.604, p=0.007), and GGT(AUC:0.593, p=0.016) in 332 patients with suspected brucellosis. Conclusions Increased levels of AST, ALT, GGT, CRP, and ESR may have a complementary role in the differential diagnosis of childhood brucellosis. However, all of these markers should be evaluated with clinical findings due to their low specificity and sensitivity.

show abstract

Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

Cited by 31 publications

References 22 publications

Epidemiological Characteristics and Spatiotemporal Trend Analysis of Human Brucellosis in China, 1950–2018

Epidemiological Characteristics and Spatiotemporal Trend Analysis of Human Brucellosis in China, 1950–2018

Revisiting Brucellosis in Small Ruminants of Western Border Areas in Pakistan

Investigation of the Sensitivity and Specificity of Laboratory Tests Used in Differential Diagnosis of Childhood Brucellosis

Contact Info

Product

Resources

About