Comparison of mycobacteria growth indicator tube with BACTEC 460 for detection and recovery of mycobacteria from clinical specimens

Badak, F Z; Kiska, Deanna L.; Setterquist, S; Hartley, Christine L.; O’Connell, Michael; Hopfer, Roy L.

doi:10.1128/jcm.34.9.2236-2239.1996

Cited by 68 publications

(37 citation statements)

References 7 publications

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“…Detection times recorded for the two culture systems were shown not to be significantly different throughout this study. This is in agreement with the findings of previous studies to compare the performance of MGIT and BACTEC for the culture of mycobacteria from specimens other than milk (Badak et al. 1997; Casal et al.…”

Section: Discussionsupporting

confidence: 93%

“…The manually read MGIT tube is a non‐radiometric alternative to the BACTEC 460TB system and its performance in comparison with this system for the culture of Mycobacterium tuberculosis and M. avium – M. intracellulare complex from clinical specimens was extensively investigated when it was first introduced (Badak et al. 1997; Casal et al.…”

Section: Introductionmentioning

confidence: 99%

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Comparative evaluation of the MGITTM and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

et al. 2003

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Aims:To compare the detection capabilities of the non-radiometric MGIT TM (Mycobacteria Growth Indicator Tubes) and radiometric BACTEC 460TB culture systems (Becton Dickinson, Cowley, Oxford, UK) for recovering Mycobacterium avium subsp. paratuberculosis from milk. Methods and Results: Ultra heat treated (UHT) milk samples spiked with different levels of M. paratuberculosis (10-10 7 cells ml )1 ) were inoculated into MGIT and BACTEC media (containing recommended supplements) with and without prior chemical decontamination of the milk samples with 0AE75% (w/v) cetylpyridinium chloride for 5 h. Time for the detection of growth in days was recorded for each culture system, and a M. paratuberculosis count for each milk sample was calculated from BACTEC readings using a published formula. Correlation between MGIT and BACTEC detection times was 0AE6983. Both culture systems were capable of detecting 10-100 M. paratuberculosis cells ml )1 in milk within 30-40 days when no decontamination treatment was applied, but only 10 2 -10 3 cells ml )1 or greater when chemical decontamination was applied before culture.Conclusions, Significance and Impact of Study: The non-radiometric MGIT system could be substituted for the radiometric BACTEC system for the culture of M. paratuberculosis from milk without loss of detection sensitivity. Chemical decontamination before culture caused a significant reduction in numbers of viable M. paratuberculosis in all spiked milk samples resulting in decreased detection capability for both culture systems.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of the MGITTM and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

et al. 2003

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“…In one of them [18] the similarity of data is particularly striking when results on smear‐positive samples (as were the majority of ours) are compared. In the other [19], characterized by a high prevalence of M. avium complex, results closer to ours are represented by the M. tuberculosis isolations. In a further study too [20], where a limited number of respiratory specimens were tested in parallel with MGIT and Löwenstein–Jensen, the results presented are in agreement with ours.…”

Section: Discussionsupporting

confidence: 89%

“…Several evaluations of MGIT in comparison with alternative media have been recently reported; one of them [17], being restricted to blood samples, is not directly comparable with our study. Two papers [18,19] which tested in parallel MGIT with BACTEC and solid media gave results compatible with ours. In one of them [18] the similarity of data is particularly striking when results on smear‐positive samples (as were the majority of ours) are compared.…”

Section: Discussionsupporting

confidence: 85%

Multicenter evaluation of mycobacteria growth indicator tube (MGIT) compared with the BACTEC radiometric method, BBL biphasic growth medium and Löwenstein—Jensen medium

Tortoli

Mandler

Tronci

et al. 1997

Clinical Microbiology and Infection

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OBJECTIVE: To evaluate the new BBL mycobacteria growth indicator tube (MGIT) in comparison with other media. METHODS: MGIT was evaluated in 10 Italian centers on 433 clinical samples, mainly of respiratory origin and mainly smear positive, in comparison with Löwenstein---Jensen and with one or more other methods represented, according to participating centers, by the BACTEC radiometric method or by the biphasic BBL Septi-Chek AFB system. While MGIT and Löwenstein---Jensen were used for all the samples, 285 of them were also inoculated in BACTEC vials and 274 in biphasic bottles. Of these samples, 132 were investigated with all the four methods. RESULTS: Although less rapid and sensitive than the radiometric method, the results of MGIT were equal when compared with the other two media with respect to overall isolation yield; furthermore, it allowed the detection of growth in significantly shorter times. CONCLUSIONS: The results of this study indicate the value of MGIT for the detection of mycobacteria and, thanks to its extreme simplicity of use, its suitability for small and large laboratories. Its combined use with a solid medium can substantially improve the diagnosis of mycobacterial infection.

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“…The major liquid medium has a Middlebrook 7H9 Broth base combined with a growth indicator system (Mycobacteria Growth Indicator Tube, MB/BacT). 15,16 Three representative solid media are the egg-based L€ owenstein-Jensen, Ogawa medium ( Fig. 3), or Middlebrook 7H10/7H11 agar medium.…”

Section: Culture Testmentioning

confidence: 99%

Laboratory procedures for the detection and identification of cutaneous non‐tuberculous mycobacterial infections

Nakanaga

Hoshino

Yotsu

et al. 2012

The Journal of Dermatology

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There is evidence that the incidence of cutaneous non-tuberculous mycobacterial (NTM) infection is increasing worldwide. Novel culture methods and new analytical procedures have led to significant advancements in understanding the origin and progression of NTM infections. Differential identification of NTM isolates is important because culture characteristics and/or sensitivity to anti-mycobacterium drugs vary between different mycobacterial species. In this manuscript, we describe the latest diagnostic techniques for cutaneous NTM infection and show how these methodologies can be used for the diagnosis of Buruli ulcer in Japan.

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Comparison of mycobacteria growth indicator tube with BACTEC 460 for detection and recovery of mycobacteria from clinical specimens

Cited by 68 publications

References 7 publications

Comparative evaluation of the MGITTM and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

Comparative evaluation of the MGITTM and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

Multicenter evaluation of mycobacteria growth indicator tube (MGIT) compared with the BACTEC radiometric method, BBL biphasic growth medium and Löwenstein—Jensen medium

Laboratory procedures for the detection and identification of cutaneous non‐tuberculous mycobacterial infections

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