Comparison of nanofiltration efficacy in reducing infectivity of centrifuged versus ultracentrifuged 263K scrapie‐infected brain homogenates in “spiked” albumin solutions

Cardone, Franco; Simoneau, Steve; Arzel, Aude; Puopolo, Maria; Berardi, Vito Angelo; Abdel‐Haq, Hanin; Galeno, Roberta; Pascalis, Angela De; Sbriccoli, Marco; Graziano, Silvia; Valanzano, Angela; Porte, Patrick; Diringer, Heino; Brown, Paul; Flan, Benoît; Pocchiari, Maurizio

doi:10.1111/j.1537-2995.2011.03425.x

Cited by 15 publications

(26 citation statements)

References 63 publications

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“…Collectively, these findings are in line with the biological properties of both S HS and plasma [9]. They suggest that the concentration of PrP Sc in the blood could be higher than reported levels.…”

Section: Soluble and Insolublesupporting

confidence: 88%

“…Lacroux et al [24] recently confirmed this possibility when they found that plasma from scrapie-infected sheep was still efficiently infectious after leukodepletion. Evidence-based studies have revealed that soluble prion infectivity is also present in the central nervous system and that it has a biophysical profile similar to that of the peripheral plasma-associated infectivity [5,9,10]. These studies showed that this neuronal infectivity is associated with an aqueous fraction of scrapie-infected hamster brain homogenate termed S HS .…”

Section: Introductionmentioning

confidence: 94%

“…This close similarity of S HS to plasma enabled the recent validation of S HS as the best prion spike for validation studies on prion removal from blood-and plasma-derived products [9,10]. In fact, when used to spike manufactured human albumin and human whole blood and red blood cells, S HS was shown to be capable of mimicking the endogenous soluble infectivity of plasma or red blood cells from 263K-infected hamsters with respect to different kinds of filtration devices.…”

Section: Introductionmentioning

confidence: 94%

“…The infectivity titer in the 263K-infected S HS , P HS , S LS , and plasma preparations that were used in this study was estimated using an endpoint titration bioassay (9,10). Hamsters used in the bioassay were housed at the animal facility of the Istituto Superiore di Sanità under the supervision of the Service for Biotechnology and Animal Welfare of the institute, which adheres to national and international regulations on animal welfare.…”

Section: Calculation Of the Reduction Factor For S Hs Infectivity Titermentioning

confidence: 99%

“…263K-infected S HS preparations containing wsPrP Sc (used in this study) were tested for infectivity in healthy hamsters and found to contain soluble infectivity that can cause illness [9]. All of the treated animals developed disease, which enabled the estimation of infectivity titers of 6.03 ± 0.18, 8.15 ± 0.01, and 8.51 ± 0.38 Log LD 50 /ml in S HS , P HS and S LS , respectively.…”

Section: S Hs Prp Sc and Magnitude Of Infectivitymentioning

confidence: 99%

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Detection of water-soluble disease-associated PrP species in blood and brain of scrapie-infected hamster

Abdel‐Haq

2015

Arch Virol

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The high-speed supernatant (S(HS)) of scrapie-infected hamster brain homogenate contains a soluble infectivity similar to that of the plasma that escapes leukodepletion and can transmit prion infection. This recent finding highlights the fact that soluble prion infectivity could be relevant for prion disease propagation and progression. PrP(Sc) is essential in prion disease pathogenesis, but little to nothing is known about the PrP(Sc) species that may be associated with this form of prion infectivity. Scrapie-infected hamster plasma and S(HS) were subjected to biochemical analysis, and the results demonstrate for the first time that soluble infectivity is associated with a water-soluble PrP(Sc) species with substantially different properties from classical PrP(Sc), the concentration of which seems to correlate with the magnitude and efficiency of the soluble infectivity. Such characteristics suggest that this species might represent the soluble prion agent itself or its vehicle, highlighting the need to adequately revise the strategies involved in prion removal, diagnosis, and therapy.

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Section: Soluble and Insolublesupporting

confidence: 88%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 94%

Section: Calculation Of the Reduction Factor For S Hs Infectivity Titermentioning

confidence: 99%

Section: S Hs Prp Sc and Magnitude Of Infectivitymentioning

confidence: 99%

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Detection of water-soluble disease-associated PrP species in blood and brain of scrapie-infected hamster

Abdel‐Haq

2015

Arch Virol

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Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data

Gröner

2023

Biotechnology Progress

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The capacity to remove viruses by Planova filters produced by Asahi Kasei, primarily by small virus‐retentive filters, were compiled from data in peer‐reviewed publications and, partly, publicly available data from presentations at conferences (Planova workshops). Data from more than 100 publications and presentations at conferences covering Planova filters were assessed. The data were grouped according to the different virus filters regarding mean pore sizes and viruses of different sizes for plasma and cell culture derived products. Planova 15N and 20N filters removed parvoviruses below the limit of detection of viruses in the filtrate in approx. 50% of all studies and mean LRFs (log reduction factors) for viruses detected in the filtrate were above 4, demonstrating effective parvovirus reduction. Parvovirus removal capacity increased for Planova BioEX filters as well as for 2 Planova 20N in series. Large viruses as retroviruses (e.g., HIV and MuLV), herpesviruses, flaviviruses and togaviruses were removed effectively by Planova 15N, 20N and BioEX filters and also by Planova 35N filters. Flow interruption, transmembrane pressure, volume and protein concentration per filter area had had no substantial impact on virus removal capacity at manufacturing specification. In conclusion, the incorporation of Planova filters in manufacturing processes of biologicals remove, depending on the filter pore size, small and large viruses from the feed stream reliably. This virus reduction step with an orthogonal mechanism integrated in the manufacturing processes of biologicals, based primarily on size exclusion of viruses, improves the virus safety of these biopharmaceutical products considerably.

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Assessment of the effect of polymeric nanoparticles on storage and stability of blood products (red blood cells, plasma, and platelet)

Mehrizi

2022

Polym. Bull.

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Comparison of nanofiltration efficacy in reducing infectivity of centrifuged versus ultracentrifuged 263K scrapie‐infected brain homogenates in “spiked” albumin solutions

Cited by 15 publications

References 63 publications

Detection of water-soluble disease-associated PrP species in blood and brain of scrapie-infected hamster

Detection of water-soluble disease-associated PrP species in blood and brain of scrapie-infected hamster

Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data

Assessment of the effect of polymeric nanoparticles on storage and stability of blood products (red blood cells, plasma, and platelet)

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