Comparison of Natural Extraction and Recombinant Mussel Adhesive Proteins Approaches

Castillo, Judit; Shanbhag, Bhuvana K.; He, Li

doi:10.1007/978-3-319-51639-4_5

Cited by 7 publications

(7 citation statements)

References 73 publications

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“…5,6 Efforts have been made in developing polypeptide-based adhesives utilizing the DOPA chemistry found in mussel adhesive proteins. 7 However, because DOPA is a noncanonical amino acid, the incorporation of DOPA into production of polypeptides often requires post-expression modification to convert tyrosine to DOPA. Direct incorporation of DOPA during recombinant mussel adhesive protein expression in Escherichia coli has been reported with good incorporation rate but low yield.…”

Section: ■ Introductionmentioning

confidence: 99%

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Comparison between Catechol- and Thiol-Based Adhesion Using Elastin-like Polypeptides

Lin

Liu

2020

ACS Appl. Bio Mater.

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Different chemistries have been utilized for adhesive materials to achieve adhesion in a humidified environment. l-3,4-dihydroxyphenylalanine (DOPA) found in marine mussel adhesive proteins has generated great interest because DOPA participates in multiple reaction mechanisms that confer the ability to adhere in wet conditions. However, the mussel adhesive complex also contains proteins with a relatively high thiol content, and these proteins can contribute to adhesion through the formation of disulfide bonds or interactions with DOPA. This work probes the individual contributions and interactions of DOPA and thiol chemistries to adhesion. To do so, we took advantage of the sequence flexibility in elastin-like polypeptides (ELPs) to create model proteins with highly similar sequences that are rich in either DOPA or thiol residues. The sequence similarity between the two ELP adhesives allowed us to focus on the differences between DOPA- and thiol-based adhesion. Curing kinetics in a wet setting, capability to recover from disturbance in the curing process, and cytocompatibility of the two adhesives were compared. Both chemistries resulted in cytocompatible materials. However, thiol chemistry had faster curing kinetics and higher adhesion strengths, whereas DOPA chemistry showed better recovery from disturbances during the curing process. By utilizing both DOPA- and thiol-based chemistry simultaneously and adding iron ions, we achieved fast curing kinetics, strong adhesion strengths, and good recovery from disturbances to curing. These insights into the contribution of these chemistries to adhesion provide important lessons for researchers designing adhesives that work in a humid environment.

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Section: ■ Introductionmentioning

confidence: 99%

“…Efforts have been made in developing polypeptide-based adhesives utilizing the DOPA chemistry found in mussel adhesive proteins . However, because DOPA is a noncanonical amino acid, the incorporation of DOPA into production of polypeptides often requires post-expression modification to convert tyrosine to DOPA.…”

Section: Introductionmentioning

confidence: 99%

Comparison between Catechol- and Thiol-Based Adhesion Using Elastin-like Polypeptides

Lin

Liu

2020

ACS Appl. Bio Mater.

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“…It seems that MAP functioned as a fast-degrading and biocompatible adhesive, which may be associated with its ingredients. MAP is composed of various amino acids commonly found in collagen, including tyrosine, the most abundant amino acid in MAP, which plays a key role in adhesion [ 14 19 ]. Because none of the amino acids involved in MAP are foreign to vertebrate systems, MAP could be completely absorbed without unwanted byproducts within a short healing period [ 18 19 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…MAP enables mussels to attach to rocks in marine environments, which are typically characterized by humidity, salinity, tides, and waves [ 9 ]. In addition, it allows adhesion to various substrates, including metal, glass, plastic, and living body substances [ 9 10 11 12 13 14 ]. MAP is rich in the amino acid tyrosine, which by enzymatic modification, is transformed to 3,4-dihydroxyphenyl-L-alanine (DOPA) [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, it allows adhesion to various substrates, including metal, glass, plastic, and living body substances [ 9 10 11 12 13 14 ]. MAP is rich in the amino acid tyrosine, which by enzymatic modification, is transformed to 3,4-dihydroxyphenyl-L-alanine (DOPA) [ 14 ]. Several studies have found the adhesive strength of MAP to be associated with its DOPA content [ 15 16 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects

Song

Kang

Kook

et al. 2018

J Periodontal Implant Sci

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PurposeThe aim of the present study was to evaluate the biocompatibility and barrier function of mussel adhesive protein (MAP)-loaded collagen membranes in guided bone regeneration (GBR).MethodsEight male New Zealand white rabbits were used. Four circular defects (diameter: 8 mm) were created in the calvarium of each animal. The defects were randomly assigned to 1) a negative control group, 2) a cyanoacrylate (CA)-loaded collagen membrane group (the CA group), 3) a MAP-loaded collagen membrane group (the MAP group), and 4) a group that received a polycaprolactone block with MAP-loaded collagen membrane (the MAP-PCL group). Specimens were harvested at 2 weeks (n=4) and 8 weeks (n=4) postoperatively for observational histology and histometric analysis.ResultsIn the histologic analysis, MAP was completely absorbed without any byproducts. In contrast, some of the CA adhesive remained, showing an inflammatory reaction, at 8 weeks. In the MAP-PCL group, the MAP-loaded collagen membranes served as a barrier membrane despite their fast degradation in GBR. No significant difference was found in the amount of new bone between the MAP-PCL and MAP groups (1.82±0.86 mm2 and 2.60±0.65 mm2, respectively).ConclusionsThe MAP-loaded collagen membrane functioned efficiently in this rabbit calvarial GBR model, with excellent biocompatibility. Further research is needed to assess clinical applications in defect types that are more challenging for GBR than those used in the current model.

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Bio‐inspired Protein‐Based and Activatable Adhesion Systems

Heinritz,

Ng,

Scheibel

2023

Adv Funct Materials

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Adhesives are in general chemically or physically sticky substances used to join surfaces. In case of gluing biological and living substrates, there is a need for bioadhesives that meet requirements such as biocompatibility, non‐toxicity, and degradability. Inspiration for bioadhesives is found in nature, where distinct mussels, sandcastle worms, barnacles, caddisfly larvae, spiders, and glowworms amongst others, use mainly protein‐based glues for various purposes. There is a great selection of reviews and books covering the use of various bioadhesives in various applications, but here the focus lies on advances in the development of bio‐inspired protein‐based adhesives for biomedical applications.

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Comparison of Natural Extraction and Recombinant Mussel Adhesive Proteins Approaches

Cited by 7 publications

References 73 publications

Comparison between Catechol- and Thiol-Based Adhesion Using Elastin-like Polypeptides

Comparison between Catechol- and Thiol-Based Adhesion Using Elastin-like Polypeptides

Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects

Bio‐inspired Protein‐Based and Activatable Adhesion Systems

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