Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects

Song, Woong Kyu; Kang, Joo Hyun; Kook, Jae; Lee, Jung Seok; Paik, Jeong Won; Jung, Ui Won; Kim, Byung Hoon; Choi, Seong‐Ho

doi:10.5051/jpis.2018.48.5.305

Cited by 10 publications

(11 citation statements)

References 34 publications

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“…Moreover, graft material was inserted within the gap between the implant surface and the bony wall [20]. A combinational approach with a connective tissue graft and guided bone regeneration was performed at the convexity area of the labial surface in the implant site [21,22]. Socket shield technique can be applied as an alternative for avoidance of buccal bone loss at immediate implant placement [23].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Alveolar Bone Morphology of the Maxillary Central and Lateral Incisors with Normal Occlusion

et al. 2019

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Background and objectives: This study investigated the morphology of the labial and palatal bony wall of the maxillary central and lateral incisors using cone-beam computed tomography (CBCT). The difference between males and females and the measurement between right and left sides were measured. Materials and Methods: Twenty participants, consisting of 11 females and 9 males having normal occlusion, were used for the analysis. The mean age was 21.9 ± 3.0 years. The thickness of the labial bony wall and palatal bony wall, perpendicular to the long axis of the root, were evaluated at 3 and 5 mm apical from the cemento-enamel junction (CEJ) and at the root apex. The available bony wall below the apex of the central and lateral incisors, and the angulation between the long axis of the tested tooth and outer surface of the labial bone were measured. Results: The mean labial bony wall thickness at the 3 and 5 mm apical from the CEJ were 1.1 ± 0.3 mm and 1.0 ± 0.4 mm for central incisors, respectively, as well as 1.2 ± 0.4 mm and 1.0 ± 0.4 mm for lateral incisors, respectively. The mean palatal bony wall thickness at 5 mm from the CEJ was above 2 mm in the central and lateral incisors. The percentage of labial bony wall thickness 2 mm or greater at the root apex in central incisors was higher than in lateral incisors (62.5% vs. 55.0%). The percentage of palatal bony wall thickness ≥2 mm at 3 mm apical from the CEJ in the central incisors was higher than in the lateral incisors (37.5% vs. 15.0%). The results on the left and right sides did not show statistically significant differences, except in the labial and palatal bony wall thickness at 3 mm from the CEJ in the lateral incisor. Generally, no significant differences were seen between males and females, but males had a significantly higher labial bony wall thickness at 3 and 5 mm from the CEJ in the central and lateral incisors when compared with females. Conclusions: This study showed that a majority of the cases of Korean participants had less than 2 mm of labial bony wall thickness at 3 and 5 mm apical from the CEJ at central and lateral incisors, and this should be kept in mind while performing dental practices, including tooth extraction or immediate implantation in anterior regions. Preoperative analysis using CBCT may be beneficial for establishing the treatment plan.

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Section: Discussionmentioning

confidence: 99%

Analysis of Alveolar Bone Morphology of the Maxillary Central and Lateral Incisors with Normal Occlusion

et al. 2019

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“…A novel method of attaching a collagen membrane and block type bone graft with MAP has been suggested for overcoming the clinical limitations of pin application. 7 The authors evaluated the bone regeneration by attaching crosslinked collagen membrane and polycaprolactone (PCL) block with MAP. The results of this study confirmed higher biocompatibility of MAP, compared to cyanoacrylate, which is a chemically synthesized bio-adhesive.…”

Section: ⅳ Discussionmentioning

confidence: 99%

“…6 For membrane fixation avoiding additional surgery for pin removal, loading mussel adhesive protein (MAP) to the membrane was suggested. 7 MAP is secreted by the mussel foot of Mytilus genus and plays a crucial role in attaching to rocks in marine environments. 8 A previous study using mussel adhesive protein for membrane fixation showed biocompatible and efficient outcomes for GBR in the rabbit calvaria model.…”

Section: ⅰ Introductionmentioning

confidence: 99%

“…8 A previous study using mussel adhesive protein for membrane fixation showed biocompatible and efficient outcomes for GBR in the rabbit calvaria model. 7 Among the composition of mussel foot protein, 3,4-dihydroxy-L-phenylalanine (DOPA), which is a hydroxylated form of tyrosine, is known to play a crucial role in adhesive property. 8 Thus, the combined use of collagenated synthetic bone graft and DOPA-loaded collagen membrane is expected to maximize the stability of grafted sites while avoiding an additional surgical approach.…”

Section: ⅰ Introductionmentioning

confidence: 99%

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Bone Regeneration Using Collagenated Synthetic Bone Graft Attached to Collagen Membrane with 3,4-dihydroxy-L-phenylalanine (DOPA)

Hong¹,

Dolgorsuren

Jeon

et al. 2021

kaomi

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The stability of graf t material and membrane is crucial for successf ul guided bone regeneration. As 3,4-dihydroxy-L-phenylalanine (DOPA) is known to contribute to the adhesiveness of natural mussel adhesive proteins, the aim of this study was to evaluate the biocompatibility and bone regeneration using collagenated synthetic bone graft attached to the collagen membrane with DOPA in the rabbit calvaria model. Materials and Methods:Four circular bone defects of 8 mm diameter were created in the rabbit calvarium. Each defect in the calvarium was randomly allocated to 1) sham control group, 2) bone graft using a particle-type synthetic bone substitute (OP) group, 3) bone graft using collagenated synthetic bone block (OC) group, or 4) bone graf t using the collagenated synthetic bone graf t attached to collagen membrane with DOPA (OC-DOPA) group. A total of 10 rabbits were sacrificed at either 2 or 8 weeks post-surgery.Results: During histometric analysis, new bone formation in the OC-DOPA group was significantly greater than that in sham control at 8 weeks. On the contrary, there was no statistically significant difference in new bone area (NBA) between OC-DOPA and OC. Histologic analysis revealed infiltrations of inflammatory cells in the OP, OC, and OC-DOPA groups at 2 weeks. At 8 weeks, there

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“…Over the last decades, several studies have attributed the MAPS coating and adhesive properties to the abundance of l-3,4-dihydroxyphenylalanine (l-DOPA); a catecholamine whose formation is derived from the hydroxylation of the aromatic ring of tyrosine residues [15]. In this way, MAPS closer to the adhesion interface have shown a higher amount of l-DOPA residues [16] and, on the contrary, the absence of l-DOPA in MAPS composition has been associated with the loss of the adhesion capacity [17]. Additionally, other studies have reported that the presence of the catechol amino-acid, DOPA, enables the binding in both organic and inorganic surfaces through H-bond or metal reversible (non-covalent) or irreversible (covalent) interactions due to the diverse chemical capabilities of the catechol; reaffirming in this way the dominant role of the catechol chemistry in the adhesion and crosslinking of MAPS ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Specific chemical incorporation of l-DOPA and functionalized l-DOPA-hyaluronic acid in Candida antarctica lipase: creating potential mussel-inspired bioadhesives

et al. 2020

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Mussel adhesives proteins have been extensively studied as a promising alternative in bioadhesives due to their ability to provide durable anchoring under different surfaces in seawater. These charasteristics have been attributed to the presence of the reduced catechol form, 3,4-dihydroxyphenylalanine (DOPA) of its structure. However, its practical applications have been limited due to drawbacks with natural extraction. Here, a novel method have been described for site-specific chemical incorporation of l-3,4-dihydroxyphenylalanine methyl ester (l-DOPAME) into proteins, in particular Candida antartica fraction B (CAL-B) lipase. Two strategies were followed, direct conjugation of DOPA at the C-terminus on the surface of the protein, and protein conjugation with tailor-made glycopolymers (DOPA-hyaluronic acid (HA) polymers) at the N-terminus. In all cases, the characterization of the new DOPA-proteins was carried out using circular dichroism, fluorescence or mass spectrometry. An improvement in the activity (in some cases more than 2 times) or the thermostability of CAL-B (with a half live 4 fold greater in some cases) was found by the incorporation of DOPA molecules. These DOPA-proteins showed excellent underwater covalent adhesive ability on amino functionalized surfaces in aqueous media compared to other modified [e.g. tyrosine modified (TYR)] CAL-B proteins. At pH 8.5, CALB-DOPA proteins were completely adsorbed after 90 min of incubation, whereas about 10% of CALB-HA or CALB-TYR proteins were adsorbed at the same time. Native CAL-B adsorption was not observed. These results suggest a potential application of these DOPA-proteins as bioglues or bioadhesives for practical underwater applications.

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Biomimetic characteristics of mussel adhesive protein-loaded collagen membrane in guided bone regeneration of rabbit calvarial defects

Cited by 10 publications

References 34 publications

Analysis of Alveolar Bone Morphology of the Maxillary Central and Lateral Incisors with Normal Occlusion

Analysis of Alveolar Bone Morphology of the Maxillary Central and Lateral Incisors with Normal Occlusion

Bone Regeneration Using Collagenated Synthetic Bone Graft Attached to Collagen Membrane with 3,4-dihydroxy-L-phenylalanine (DOPA)

Specific chemical incorporation of l-DOPA and functionalized l-DOPA-hyaluronic acid in Candida antarctica lipase: creating potential mussel-inspired bioadhesives

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