Comparison of neuroplastin and synaptic marker protein expression in acute and cultured organotypic hippocampal slices from rat

Buckby, Lucy E.; Mummery, Rosemary S.; Crompton, M. R.; Beesley, Philip; Empson, Ruth M.

doi:10.1016/j.devbrainres.2004.01.006

Cited by 24 publications

(20 citation statements)

References 31 publications

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“…Dissociated hippocampal neurones were prepared as previously described [23,24] from embryonic day 18 hippocampi maintained for 18-28 days. Hippocampal neurons cells were grown at 37 °C in 5% CO 2 in DMEM GlutaMax (Invitrogen, Carlsbad CA, USA) medium supplemented with 10% FBS, 100 µg/mL Pen-Strep, 25 µg/mL zeocin, 5 µg/mL blasticidin and 25 µg/mL hygromycin.…”

Section: Cell Culture Methodsmentioning

confidence: 99%

Tracking of fast moving neuronal vesicles with ageladine A

Bickmeyer¹,

Heisenberg²,

Podbielski³

et al. 2010

Biochemical and Biophysical Research Communications

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Tracking of fast moving neuronal vesicles with ageladine A

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Section: Cell Culture Methodsmentioning

confidence: 99%

Tracking of fast moving neuronal vesicles with ageladine A

Bickmeyer¹,

Heisenberg²,

Podbielski³

et al. 2010

Biochemical and Biophysical Research Communications

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“…Generally they are cultured in vitro for 2-3 weeks before being utilised, but they can be maintained for several months (De Simoni and Yu 2006;Gogolla et al 2006a). Organotypic brain slices have proven to be a good model for the intact brain as they develop in a similar fashion and their architecture is largely maintained (De Simoni et al 2003;Gogolla et al 2006b;Gähwiler et al 1997;Buckby et al 2004;Noraberg et al 2005).…”

Section: Introductionmentioning

confidence: 97%

Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices

Bakmand

Troels-Smith

Nissen

et al. 2015

Biomed Microdevices

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Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.

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“…Because the predicted MW of LRRK2 is ϳ286 kDa, we considered and analyzed the band at ϳ280 kDa as endogenous full-length LRRK2. Interestingly, LRRK2 levels were positively associated with synapse maturation, as indicated by the increasing level of synaptophysin and PSD-95 proteins (Verderio et al, 1999;Buckby et al, 2004;Mielke et al, 2005) (Fig. 1 A, B).…”

Section: Lrrk2 Is Positively Associated To Neuron Maturationmentioning

confidence: 99%