Comparison of nitrite/nitrate concentration in human plasma and serum samples measured by the enzymatic batch Griess assay, ion-pairing HPLC and ion-trap GC–MS: The importance of a correct removal of proteins in the Griess assay

Romitelli, Federica; Santini, Stefano Angelo; Chierici, Eleonora; Pitocco, Dario; Tavazzi, Barbara; Amorini, Angela Maria; Lazzarino, Giuseppe; Stasio, Enrico Di

doi:10.1016/j.jchromb.2007.02.003

Cited by 109 publications

(63 citation statements)

References 33 publications

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“…During the last 10 years this tecnique was found to induce an immediate anthalgic and anti-inflammatory effect and a long-term tissue regeneration together with increase of angiogenesis [5][6][7][8][9]16]. One of the possible mechanisms of action of the anti-inflammatory effect of ESWT may be related to the ability of ESW to keep local NO contents at a physiological level in the early phase of inflammatory response, enhancing either a non-enzymatic or enzymatic production of NO [15]. Therefore induction of NO 9 synthesis has been suggested to be one of the most important mechanism implicated in the anti-inflammatory effect of ESWT, while increased expression of VEGF and the consequent mobilization of endothelial progenitor cells can explain the proangiogenic action [6][7][8]17].…”

Section: Discussionmentioning

confidence: 99%

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Effects of shock wave therapy in the skin of patients with progressive systemic sclerosis: a pilot study

et al. 2010

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Results: After ESWT we observed a rapid and persistent reduction of RSS and decrease of VAS. There was no difference in skin thickness before and after ESWT; however we observed a more regular skin structure and an improvement in skin vascularization 90 days after treatment. EPCs and CECs increased 60 and 90 days after treatment, while serological biomarkers showed no variation before and after therapy. Conclusions: ESWT resulted in an improvement of VAS, RSS and of skin vascularscore. An increase of CECs and EPCs was also observed after therapy.

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Section: Discussionmentioning

confidence: 99%

“…Enzymatic Griess assay on deproteinated serum was used to determine nitric oxide values (kit Cayman Chemical; Ann Arbor, Michigan, USA) [15].…”

Section: Nitric Oxide Evaluationmentioning

confidence: 99%

Effects of shock wave therapy in the skin of patients with progressive systemic sclerosis: a pilot study

et al. 2010

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“…Recently, it has been demonstrated that the Griess assay, supplemented with an appropriate deproteinization procedure of the serum sample, can be successfully applied for NO metabolite measurement. This procedure allows to obtain NOx levels in agreement with CG-MS and HPLC [21].…”

Section: Abbreviationsmentioning

confidence: 99%

Oxidative Stress, Nitric Oxide, and Diabetes

Pitocco¹,

Zaccardi²,

Stasio³

et al. 2010

Rev Diabet Stud

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288

194

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■ AbstractIn the recent decades, oxidative stress has become focus of interest in most biomedical disciplines and many types of clinical research. Increasing evidence from research on several diseases show that oxidative stress is associated with the pathogenesis of diabetes, obesity, cancer, ageing, inflammation, neurodegenerative disorders, hypertension, apoptosis, cardiovascular diseases, and heart failure. Based on this research, the emerging concept is that oxidative stress is the "final common pathway", through which risk factors of several diseases exert their deleterious effects. Oxidative stress causes a complex dysregulation of cell metabolism and cell-cell homeostasis. In this review, we discuss the role of oxidative stress in the pathogenesis of insulin resistance and beta-cell dysfunction. These are the two most relevant mechanisms in the pathophysiology of type 2 diabetes, and in the pathogenesis of diabetic vascular complications, the leading cause of death in diabetic patients.

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“…Plasma was deproteinated using an acetonitrile/chloroform treatment based on a protocol from Romitelli and colleagues (Romitelli et al, 2007). Plasma was mixed 1:1 with acetonitrile, vortexed for 60s and centrifuged at 21,000g for 10min at 4°C.…”

Section: Plasma Nitrate Plus Nitrite (No X ) Determinationmentioning

confidence: 99%

Phenylhydrazine-induced anemia causes nitric-oxide-mediated upregulation of the angiogenic pathway in Notothenia coriiceps

Borley

Beers

Sidell

2010

Journal of Experimental Biology

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SUMMARYAntarctic icefishes possess several cardiovascular characteristics that enable them to deliver oxygen adequately in the absence of hemoglobin (Hb). To gain insight into mechanisms driving development of these cardiovascular characteristics of icefish, we chemically induced severe anemia in a red-blooded notothenioid, Notothenia coriiceps. After 10 days of treatment with phenylhydrazine HCl, the hematocrit and Hb concentration of N. coriiceps decreased by >90% and >70%, respectively. Anemic fish exhibited a significantly higher concentration of nitric oxide (NO) metabolites in their plasma compared with that of control animals, indicating that corporeal levels of NO are higher in anemic animals than in control fish. The activity of nitric oxide synthase (NOS) was measured in brain, retina, pectoral muscle and ventricle of control and anemic animals. With the exception of retina, no significant differences in NOS activities were observed, indicating that the increase in plasma NO metabolites is due to loss of Hb, which normally plays a major role in the degradation of NO, and not due to an overall increase in the capacity for NO production. To determine whether loss of Hb can stimulate remodeling of the cardiovascular system, we measured expression of HIF-1a, PHD2 and VEGF mRNA in retinae of control and anemic fish. Expression of all three genes was higher in anemic animals compared with control N. coriiceps, suggesting a causative relationship between loss of Hb and induction of angiogenesis that probably is mediated through nitric oxide signaling.

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Comparison of nitrite/nitrate concentration in human plasma and serum samples measured by the enzymatic batch Griess assay, ion-pairing HPLC and ion-trap GC–MS: The importance of a correct removal of proteins in the Griess assay

Cited by 109 publications

References 33 publications

Effects of shock wave therapy in the skin of patients with progressive systemic sclerosis: a pilot study

Effects of shock wave therapy in the skin of patients with progressive systemic sclerosis: a pilot study

Oxidative Stress, Nitric Oxide, and Diabetes

Phenylhydrazine-induced anemia causes nitric-oxide-mediated upregulation of the angiogenic pathway in Notothenia coriiceps

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