Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Mégraud, Françis

doi:10.1016/j.jpeds.2004.10.044

Cited by 104 publications

(116 citation statements)

References 35 publications

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“…[25][26][27] After the children fasted for Ն1 hour, a baseline breath sample was collected in the manufacturer's breath collection bag. The participant then ingested 3 g of Pranactin-Citric (75 mg of 13 C-urea, citric acid, aspartame, and mannitol) reconstituted in 100 mL of bottled water.…”

Section: Detection Of H Pylorimentioning

confidence: 99%

“…This method has not been shown to cause false-positive results in pediatric populations as a result of urease-producing oral flora. 27,28 Breath samples were stored at room temperature for 1 to 4 days before being transported to Anchorage for analysis. The urea hydrolysis rate was determined using the UbiT-IR300 mass spectrophotometer (Otsuka Pharmaceutical Co, Ltd, Tokyo, Japan).…”

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Endemic Iron Deficiency Associated With Helicobacter pylori Infection Among School-Aged Children in Alaska

et al. 2006

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OBJECTIVES. Rural Alaska Natives have a high prevalence of iron deficiency and Helicobacter pylori infection. The objective of this study was to estimate the prevalence of iron deficiency, iron-deficiency anemia, and active H pylori infection among school-aged children in rural Alaska.METHODS. We enrolled 68% (688) of the 7-to 11-year-old children from 10 predominantly Alaska Native villages in southwestern Alaska. We collected venous blood samples to assess iron deficiency and anemia. Each child was tested for active H pylori infection by 13 C-urea breath test (UBT). Evaluated risk factors included age, gender, village of residence, number of household members, number of household members who were younger than 5 years, recent antibiotic use, and household water source.RESULTS. Of 688 enrolled children, iron deficiency was present in 38%, iron-deficiency anemia was present in 7.8%, and H pylori infection by UBT was present in 86%. Iron deficiency was independently associated with living in a household with Ͼ6 people and village of residence. H pylori infection by UBT was independently associated with child's age Ն10 years and village of residence. Ninety-one percent of children with iron deficiency had H pylori infection by UBT, and children with active H pylori infection were more likely to be iron deficient than uninfected children. Children with H pylori infection by UBT were also more likely to have iron-deficiency anemia than uninfected children.CONCLUSIONS. In this study of nearly 700 children in 10 different villages in Alaska, we confirmed that the high prevalence of iron deficiency persists among schoolaged children. We found that active H pylori infection was independently associated with iron deficiency and iron-deficiency anemia among children in this region. H pylori infection may account for a portion of the iron deficiency and iron-deficiency anemia in rural Alaska and other areas with high prevalences of both conditions. Innovative approaches are critically needed to address the iron deficiency in high prevalence areas such as rural Alaska and most of the developing world. Dr Baggett assisted with study design, was the primary person responsible for study implementation during the first study year, conducted all data analysis, and was the primary manuscript author. Dr Gessner was the study primary investigator, wrote the study protocol, was the primary person responsible for study implementation during the second study year, and assisted with data analysis and preparing the manuscript. Dr Parkinson was responsible for all components of laboratory analysis, assisted with study design, and provided critical input into the final manuscript. Ms Muth assisted with study design and writing the study protocol, was the primary person responsible for administrative project management, and reviewed the final manuscript. Dr Gold contributed to the study design, data analysis, and interpretation and provided critical input into the final manuscript. 2 The prevalence of iron deficiency among children...

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Section: Detection Of H Pylorimentioning

confidence: 99%

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confidence: 99%

Endemic Iron Deficiency Associated With Helicobacter pylori Infection Among School-Aged Children in Alaska

et al. 2006

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“…UBT requires patient cooperation such as rinsing their mouths with water immediately after ingestion of 13 C-urea to reduce the effect of oral urease producing bacteria. Therefore, it is more difficult to perform UBT in infants and toddler patients, and lower specificity has been obtained in children younger than 6 years old in past studies [18] [24]- [29].…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of <i>Helicobacter pylori</i> Infection in Children

Ikuse¹,

Kudo²,

Obayashi³

et al. 2017

AiM

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Proper diagnosis in the pediatric population is required to eradicate Helicobacter pylori (H. pylori) and prevent gastric cancer. Our aim was to assess the performance of non-invasive tests to diagnose H. pylori infection in pediatric patients. A retrospective analysis was performed on 141 pediatric patients requiring endoscopic evaluation and diagnostic tests for H. pylori infection to define the cause of abdominal symptoms. Non-invasive tests included the 13 C-urea breath test (UBT), a monoclonal stool antigen test using enzymelinked immuno-sorbent assay (mSAT), and a serum immunoglobulin G antibody test using antigens derived from Japanese individuals (S-Ab). This study investigated sensitivity, specificity, likelihood ratios for a positive and a negative test (LR + and LR − ), and accuracy of non-invasive tests, in comparison with invasive tests. Eighty two of 141 patients (58%) were recognized as H. pylori positive by invasive methods. When UBT, mSAT or S-Ab were analyzed, all were found to be effective over 94% accurate. Specificity ranged between 86.7% and 95.8%, and sensitivity ranged between 93.8% and 97.1%. When subjects were stratified into three distinct age groups, the best performance was achieved for 1 -6 years old with mSAT at 100% for sensitivity, specificity, and accuracy. S-Ab yielded the best results for children 7 -12 years old and the UBT test performed best for 13 -18 years old. These results demonstrate the utility of UBT, mSAT, and S-Ab non-invasive tests in diagnosing H. pylori but suggest that certain tests may be optimal for children of distinct ages. Three non-invasive tests, UBT, mSAT and S-Ab showed sufficient sensitivity, specificity and accuracy for the initial diagnosis of H. pylori infection among pediatric patients. Non-invasive tests may contribute to achieving minimum invasive diagnosis with combining with a histological test and a culture test in children.

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“…Finally, the test results were expressed on the LCD of the analyzer in a numeric fashion (0: patient not infected, 1: borderline result, or 2: patient infected); these scores corresponded to radioactivity as count per minute (CPM): <25 CPM: patient not infected, 25-50 CPM: borderline result, and >50 CPM: patient infected. We considered grades 0 and 1 as negative results for our study, and only samples with activities that were more than 50 CPM were regarded as positive (11,12).…”

Section: Methodsmentioning

confidence: 99%

Comparison of standard and standard plus vitamin E therapy for Helicobacter pylori eradications in children

Tümgör

Baran²,

Çakır³

et al. 2015

Turk J Gastroenterol

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Background/Aims: Although various drugs can be used in adults for Helicobacter pylori eradication in adults, treatment options are limited in children. The aim of this study was to compare the effects of the standard lansoprazole, amoxicillin, and clarithromycin (LAC) protocol to those of LAC + vitamin E (LACE) combination for H. pylori eradication. Materials and Methods: The study included 90 children (age range: 10-17 years) who were admitted to four pediatric gastroenterology centers between March 2011 and November 2012 with dyspeptic symptoms and who had tested positive for H. pylori by 14C-urea breath tests. The patients were randomized into two groups. The LAC group [45 patients (pts)] was treated with a standard regimen consisting of lansoprazole (1 mg/kg/day), amoxicillin (50 mg/kg/ day), and clarithromycin (14 mg/kg/day), each of which was given in two equally divided doses every 12 h for 14 days; the LACE group (45 pts) was given the standard regimen and vitamin E at 200 IU/day for 14 days. H. pylori eradication was assessed using the 14C-UBT in the 6th week after the cessation of treatment. Results: H. pylori was eradicated in 21 (46.6%) pts in the LAC group, while it was eradicated in 29 (64.4%) pts in the LACE group. There was no statistical difference between the two groups (p=0.13). Conclusion: The eradication rate of H. pylori in children while using the LAC regimen has decreased in the last years. The LACE regimen has been associated with an increased eradication rate but can reach to statistically significance. Further studies with larger cohorts are needed to examine the success of the LACE regimen for H. pylori eradication.

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Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Cited by 104 publications

References 35 publications

Endemic Iron Deficiency Associated With Helicobacter pylori Infection Among School-Aged Children in Alaska

Endemic Iron Deficiency Associated With Helicobacter pylori Infection Among School-Aged Children in Alaska

A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of <i>Helicobacter pylori</i> Infection in Children

Comparison of standard and standard plus vitamin E therapy for Helicobacter pylori eradications in children

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Comparison of non-invasive tests to detect Helicobacter pylori infection in children and adolescents: Results of a multicenter European study

Cited by 104 publications

References 35 publications

Endemic Iron Deficiency Associated With Helicobacter pylori Infection Among School-Aged Children in Alaska

Endemic Iron Deficiency Associated With Helicobacter pylori Infection Among School-Aged Children in Alaska

A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of &lt;i&gt;Helicobacter pylori&lt;/i&gt; Infection in Children

Comparison of standard and standard plus vitamin E therapy for Helicobacter pylori eradications in children

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A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of <i>Helicobacter pylori</i> Infection in Children