Comparison of optimal conditions for mass production of carboxymethylcellulase by Escherichia coli JM109/A-68 with other recombinants in pilot-scale bioreactor

Kim, Myung–Hwan; Gao, Wa; Chung, Chong-Pyoung; Lee, Jin Woo

doi:10.1007/s12257-017-0035-1

Cited by 2 publications

(9 citation statements)

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“…The production of the CMCase by B. velezensis A-68 under optimized conditions was 83.8 U/mL, whereas that by E. coli KACC 91335P was 880.2 U/mL [16]. The production by the recombinant E. coli stain was 10.5 times higher than that by the wild of Bacillus strain.…”

Section: Introductionmentioning

confidence: 88%

“…The CMCase produced by B. velezensis A-68 seemed to be essential for its survival, whereas that by E. coli KACC 91335P was not essential for its cell growth. The CMCase produced by B. velezensis A-68 was a growth-associated product, whereas that by E. coli KACC 91335P was a mixed-growth-associated product [16]. The optimal conditions for cell growth of E. coli KACC 91335P were different from its production of CMCase.…”

Section: Introductionmentioning

confidence: 95%

“…Starter cultures were prepared by transferring cells of E. coli KACC 91335P from agar slants to 120 mL of medium in 500 mL Erlenmeyer flasks [16]. They were incubated at 37 • C for 48 h in the shaking incubator for the maintenance of aerobic conditions.…”

Section: Production Of Cmcase By E Coli Kacc 91335pmentioning

confidence: 99%

“…Each resulting culture was used for an inoculum to produce CMCase by E. coli KACC 91335P. The production of CMCase by E. coli KACC 91335P were carried in 7 L bioreactors and 100 L pilot-scale bioreactors (Ko-Biotech Co., Inchen, Korea) [16]. The type of 7 and 100 L bioreactors used in this study was the typical stirred tank fermenter.…”

Section: Production Of Cmcase By E Coli Kacc 91335pmentioning

confidence: 99%

“…The optimal conditions of the aeration rate and agitation speed of 7 L bioreactors for the cell growth of E. coli KACC 91335P were 500 rpm and 0.50 vvm, respectively. However, those for the production of CMCase by E. coli KACC 91335P were 416 rpm and 0.95 vvm [16]. For the enhanced production of CMCase, there should be enough cells of E. coli KACC 91335P to participate in the production of CMCase.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Production of Carboxymethylcellulase by Recombinant Escherichia coli Strain from Rice Bran with Shifts in Optimal Conditions of Aeration Rate and Agitation Speed on a Pilot-Scale

Park

Lee

et al. 2019

Applied Sciences

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The optimal conditions including the aeration rate and agitation speed of bioreactors for the production of carboxymethylcellulase (CMCase) by a recombinant Escherichia coli KACC 91335P, expressing CMCase gene of B. velezensis A-68, were different from those for its cell growth. The enhanced production of CMCase by E. coli KACC 91335P with the conventional multistage process needs at least two bioreactors. Shifts in the optimal conditions of the aeration rate and agitation speed of the bioreactor from the cell growth of E. coli KACC 91335P to those for its production of CMCase were investigated for development of the simple and economic process with the high productivity and low cost. The production of CMCase by E. coli KACC 91335P with shifts in the optimal conditions of the aeration rate and agitation speed from the cell growth to its production of CMCase in a 100 L pilot-scale bioreactor was 1.36 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for the production of CMCase and it was even 1.54 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for cell growth. The best time for the shift in the optimal conditions was found to be the mid-log phase of cell growth. Owing to the mixed-growth-associated production of CMCase by E. coli KACC 91335P, shifts in the optimal conditions of the aeration rate and agitation speed of bioreactors from the cell growth to its production of CMCase seemed to result in relatively more cells for the participation in its production of CMCase, which in turn enhanced its production of CMCase. The process with a simple control for shifts in the aeration rate and agitation speed of a bioreactor for the enhanced production of CMCase by E. coli KACC 91335P on the pilot-scale can be directly applied to the industrial-scaled production of cellulase.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 95%

Section: Production Of Cmcase By E Coli Kacc 91335pmentioning

confidence: 99%

Section: Production Of Cmcase By E Coli Kacc 91335pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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