Comparison of Pathogenicity and Nucleotide Sequences of 3′-terminal Regions of Bean yellow mosaic virus Isolates from Gladiolus

Wada, Yukio; Iwai, Hisashi; Ogawa, Yoshihiro; Arai, K.

doi:10.1007/pl00012976

Cited by 20 publications

(6 citation statements)

References 19 publications

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“…2). Since BYMV isolates were divided into 4 pathotypes based on differential host reactions, which correlated with major subgroupings observed in phylogenetic trees based on CP aa or nt sequence (Wada, 2000). It is noteworthy that isolates MB4 and S both originated from broad bean, whereas GDD was isolated from gladiolus, so it will be of interesting if the biological properties of BYMV-Fr are different from those of other BYMV strains.…”

Section: Resultsmentioning

confidence: 82%

“…Although the genetic variability in the 3' noncoding region (NCR) has been correlated significantly with flowering plant origin of isolates (Wada et al, 2000), the most useful open reading frame of potyviral genome is the coat protein (CP) gene for taxonomic analysis of BYMV isolates (Ganesh-Selvaraj et al, 2009;Hammond and Hammond, 2003;Kumar et al, 2009;Parrella and Lanave, 2009). The symptom determinants of BYMV have been assumed to localize in the N-terminal part of the CP, one of the most hypervariable regions of the potyvirus genome (Shulka et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…Based on the results of some studies (Chang et al, 1988a;1988b), BYMV genome is initially translated into a single polyprotein which is processed by viral-encoded proteases into its mature functional products. This gene expression strategy is defined as a general scheme of potyviral gene expression based on the analyses of other members in the genus Potyvirus (Allison et al, 1986;Dougherty et al, 1989;Shukla et al, 1994;Vance et al, 1992).Although the genetic variability in the 3' noncoding region (NCR) has been correlated significantly with flowering plant origin of isolates (Wada et al, 2000), the most useful open reading frame of potyviral genome is the coat protein (CP) gene for taxonomic analysis of BYMV isolates (Ganesh-Selvaraj et al, 2009;Hammond and Hammond, 2003;Kumar et al, 2009;Parrella and Lanave, 2009). The symptom determinants of BYMV have been assumed to localize in the N-terminal part of the CP, one of the most hypervariable regions of the potyvirus genome (Shulka et al, 1988).…”

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confidence: 99%

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The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

Choi¹,

Yoon²,

Ryu³

et al. 2013

Research in Plant Disease

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Bean yellow mosaic virus (BYMV; genus Potyvirus, family Potyviridae) causes severe losses to various legume species and a number of non-legume species, particularly freesia plants. In a survey of virus diseases in Gyeonggi province, Korea, BYMV isolates were identified from many cultivated freesia species. Here, we determined the complete nucleotide sequences of a BYMV freesia isolate (BYMV-Fr; accession number FJ492961). BYMV-Fr genome consists of 9,545 nucleotides (nt) excluding the poly (A) tail and encodes 3,057 amino acid (aa), with an AUG start and UAG stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of BYMV-Fr was divided to ten proteins and the cleavage sites of each protein were determined. The coat protein (CP) and polyprotein of BYMV-Fr were compared at the aa level with those of the previously reported 4 BYMV isolates. BYMV-Fr shared 90.1 to 97.1 and 91.0 to 92.5% at the CP and polyprotein homology. Interestingly, BYMV-Fr showed identities of a lower level at the nt level of 5' noncoding region (61.4 to 67.6%) and at the aa level of P1 (71.4 to 72.8%), comparing with four BYMV isolates. Based on the aa sequence diversity of CP and polyprotein, phylogenetic analysis with the four BYMV isolates showed two distinct groups and BYMV-Fr and most BYMV isolates were most closely related to the clover yellow vein virus among 52 potyviruses. To our knowledge, this is the first report of the complete genome sequence of BYMV freesia strain.Keywords : Bean yellow mosaic virus, Freesia, Genetic diversity, Potyvirus, RT-PCR, Sequence analysis IntroductionFreesia is a small genus of southern African Iridaceae subfamily Ixioideae, which has been familiar to horticulturists and valued by them for the beauty and fragrance of the flowers. Freesia was first grown in Europe in the mid-18th century and become one of the most popular plants in horticulture in the last half of the 19th century. Appealing shapers of freesias make them suitable line flowering for any arrangement, and their wide range of color increases their versatility (Wang, 2006), resulting that freesias are excellent cut flowers. The flowers are popular used for weddings and make fragrant additions to bouquets and body flowers. Freesias are known to be infected by Bean yellow mosaic virus (BYMV), Freesia mosaic virus, Cucumber mosaic virus, Tobacco rattle virus and ophioviruses (Brunt, 1995;Vaira et al., 2006). BYMV belongs to the genus Potyvirus in the family Potyviridae and the virus is distributed worldwide (Bos, 1970). In comparison with the majority of potyviruses, BYMV has a relatively broad range of plant species. BYMV infects beans, peas, lupins, clovers and dicotyledonous ornamental crops such as gladiolus, bulbous iris, gentians, and some orchids. In particular, BYMV severely affects plant growth, yield and quality of flowers and corms in freesias (Derks et al., 1980;Magie and Poe, 1972). Many isolates of BYMV are recognized and have been divided into a BYMV subgroup along with the closely related ...

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Section: Resultsmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

Choi¹,

Yoon²,

Ryu³

et al. 2013

Research in Plant Disease

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“…Taken together, these data suggest an influence of some host plants (potato and pepper) but not others (tomato and tobacco) in the selection and evolution of PVY isolates. There are also partial arguments for host-range variations according to the source plants for strains of BYMV (Wada et al ., 2000 ) and TuMV (e.g. Stavolone et al ., 1998 ).…”

Section: Discussionmentioning

confidence: 99%

Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses

Moury

Morel

Johansen

et al. 2002

Journal of General Virology

107

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The modes of evolution of the proteins of Potato virus Y were investigated with a maximumlikelihood method based on estimation of the ratio between non-synonymous and synonymous substitution rates. Evidence for diversifying selection was obtained for the 6K2 protein (one amino acid position) and coat protein (24 amino acid positions). Amino acid sites in the coat proteins of other potyviruses (Bean yellow mosaic virus, Yam mosaic virus) were also found to be under diversifying selection. Most of the sites belonged to the N-terminal domain, which is exposed to the exterior of the virion particle. Several of these amino acid positions in the coat proteins were shared between some of these three potyviruses. Identification of diversifying selection events in these different proteins will help to unravel their biological functions and is essential to an understanding of the evolutionary constraints exerted on the potyvirus genome. The hypothesis of a link between evolutionary constraints due to host plants and occurrence of diversifying selection is discussed.

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“…On the contrary, isolates in the general group were identified in wild and domesticated hosts of monocots and dicots [3]. Some members of the monocot group can infect dicotyledonous species from three families: Orchidaceae, Iridaceae, Gentianaceae [5][6][7][8][9]. As phylogenetic relationships among BYMV isolates correlate with their natural hosts, Wylie et al (2008) proposed seven BYMV phylogenetic groupings based on coat protein sequences and the original hosts of the isolates: (i) general group with a broad host range of monocots and dicots; (ii) broad bean group; (iii) canna group; (iv) lupine group; (v) monocot group; (vi) pea group; and (vii) one strain 'W' from L. albus [3].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Bean Yellow Mosaic Virus Isolates From Gladioli in Ukraine

Dunich¹,

Sovinska²,

Dashchenko³

et al. 2022

BTSNUKSB

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Bean yellow mosaic virus (BYMV) is the one of most prevalent and harmful viruses infecting gladiolus plants worldwide. The aim of the study was to perform phylogenetic analysis of two Ukrainian BYMV isolates from gladioli grown in different regions of Ukraine and determine phylogenetic relationships of the isolates to other BYMVs. Visual diagnostics, enzyme-linked immunosorbent assay in DAS-ELISA modification, total RNA extraction, RT-PCR, phylogenetic analysis and statistical data analysis were used in this research. The results of the studies showed that gladioli of variety Pamyat’ (Poltava region, 2018) and var. Galyna Zelenobirska (Kyiv region, 2020) with symptoms of chlorotic stripes and mosaics on the leaves, flower color breaks are infected by BYMV. Fragments of the CP gene sequence of Ukrainian gladiolus isolates of bean yellow mosaic virus, named GlMP-18 (MK416160) and BYMV-Gl-SV-20 (MZ286966) were sequenced and deposited to the NCBI GenBank. Nucleotide sequences of these isolates corresponding to 578 nt of the coat protein gene (CP) located at the position 8727–9305 of the viral genome and amino acid sequences were compared with 40 known BYMV isolates/strains. Phylogenetic analysis demonstrated that GlMP-18 and BYMV-Gl-SV-20 have identity of nucleotide sequence 100% and amino acid sequence 100% with each other. Both Ukrainian isolates clustered with the Monocot group. The identity of the СР gene sequences of the two gladiolus isolates GlMP-18 and BYMV-Gl-SV-20 from geographically remote regions of the country and in different years of selection indicates a common origin of isolates and probable their distribution of planting material.

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Comparison of Pathogenicity and Nucleotide Sequences of 3′-terminal Regions of Bean yellow mosaic virus Isolates from Gladiolus

Cited by 20 publications

References 19 publications

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses

Evidence for diversifying selection in Potato virus Y and in the coat protein of other potyviruses

Phylogenetic Analysis of Bean Yellow Mosaic Virus Isolates From Gladioli in Ukraine

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