Comparison of peptidase activities in some fungi pathogenic to arthropods

Persson, Mikael; Häll, Lena; Söderhäll, Kenneth

doi:10.1016/0022-2011(84)90032-6

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…Amongst EPF, M. anisopliae produce multiple protease isozymes during the host-pathogen interaction (St. Leger, 1995). However, there is ultrastructural evidence that the degradative effects of the pathogen enzymes are limited to the vicinity of conidia (Zacharuk, 1970), mucus (Brey et al, 1986) and germ tubes (Persson et al, 1984). Ironically, fungal spore associated enzymes have received little attention despite of their vital role.…”

Section: Discussionmentioning

confidence: 98%

Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases

Qazi

Khachatourians

2007

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 98%

Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases

Qazi

Khachatourians

2007

Journal of Invertebrate Pathology

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“…Fungal and oomycete pathogens on insects and crustaceans secrete several types of lytic enzymes, including lipases, proteases and chitinases (Unestam 1966, Söderhäll & Unestam 1975, Persson et al 1984, Samuels et al 1989, Clarkson & Charnley 1996, Dieguez-*Corresponding author. E-mail: lage.cerenius@ebc.uu.se Uribeondo & Cerenius 1998, Vilcinscas & Götz 1999.…”

Section: Introductionmentioning

confidence: 99%

Analysis of chitinase expression in the crayfish plague fungus Aphanomyces astaci

Andersson¹,

Cerenius²

2002

Dis. Aquat. Org.

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Chitinase, as determined by enzymatic activity in the growth medium and by transcription of the chitinase gene AaChi1, is expressed at a high level during vegetative growth of the crayfish pathogen Aphanomyces astaci and expression is not further stimulated by chitin. Expression is not detected in zoospores and it does not increase to high levels until late during germination. Upon sporulation, chitinase expression increases to levels comparable with those seen in fast-growing mycelium. This pattern of chitinase expression is a common feature of strains representing all currently known genotypes of A. astaci, suggesting that it is an adaptation to the exclusively parasitic life-style of this species. In contrast, other Aphanomyces spp. including the saprophytes, A. laevis and A. stellatus, produce significant amounts of chitinase only in the presence of chitin. The pattern of chitinase expression is one of very few qualitative physiological characteristics known which can distinguish A. astaci from other parasitic and saprophytic species and may thus be of practical use for identification.

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“…Once the spore has started the hyphal growth phase in the host animal cuticle, it produces a continuous release of chitinase (Söderhäll, Svensson & Unestam ; Anderson & Cerenius ) and also other enzymes (Söderhäll & Unestam ; Söderhäll et al . ; Persson, Häll & Söderhäll ) that may be involved in the pathogenic process (Di≐guez‐Uribeondo & Cerenius , Bangyeekhun, Cerenius & Söderhäll ). Except the reported differences in the chitinase genes between the genotypes As and Ps1 (Makkonen et al …”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that A. astaci possesses the ability for repeated zoospore emergence, which can enhance its possibilities to locate a suitable host animal (Cerenius & S€ oderh€ all 1984a(Cerenius & S€ oderh€ all , 1985, as well as chemotaxis towards nutrients (Cerenius & S€ oderh€ all 1984b). Once the spore has started the hyphal growth phase in the host animal cuticle, it produces a continuous release of chitinase (S€ oderh€ all, Svensson & Unestam 1978;Anderson & Cerenius 2002) and also other enzymes (S€ oderh€ all & Unestam 1975;S€ oderh€ all et al 1978;Persson, H€ all & S€ oderh€ all 1984) that may be involved in the pathogenic process (Di_ eguez-Uribeondo & Cerenius 1997, Bangyeekhun, Cerenius & S€ oderh€ all 2001. Except the reported differences in the chitinase genes between the genotypes As and Ps1 (Makkonen et al2012a), variations in virulence factors between the genogroups have not been studied extensively so far.…”

Section: Discussionmentioning

confidence: 99%

Variation in the hyphal growth rate and the virulence of two genotypes of the crayfish plague organism Aphanomyces astaci

Viljamaa-Dirks

Heinikainen

Virtala

et al. 2015

Journal of Fish Diseases

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Crayfish plague, a devastating disease of freshwater crayfish, is caused by an oomycete organism, Aphanomyces astaci. Currently five genotypes of A. astaci are known, but variable features between the strains or genotypes have not been studied extensively. This study analysed 28 isolates of the As genotype and 25 isolates of the Ps1 genotype and reveals that the radial growth rate is significantly (P < 0.001) different between these two genotypes, although highly variable inside the genotype As. Two Ps1 genotype isolates and two As genotype isolates with different radial growth rates were tested in an infection trial. Clear differences were detected in the development of mortality in the test groups. The representatives of the Ps1 genotype caused total mortality within a short time span. The As genotype isolates were much less virulent. The slow-growing As isolate showed higher virulence than the As isolate with a high growth capacity. Although slow growth could be one survival strategy of the pathogen, several other mechanisms are involved in the pathogenicity and warrant further studies.

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Comparison of peptidase activities in some fungi pathogenic to arthropods

Cited by 14 publications

References 27 publications

Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases

Hydrated conidia of Metarhizium anisopliae release a family of metalloproteases

Analysis of chitinase expression in the crayfish plague fungus Aphanomyces astaci

Variation in the hyphal growth rate and the virulence of two genotypes of the crayfish plague organism Aphanomyces astaci

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