Comparison of PET Imaging with 64Cu-Liposomes and 18F-FDG in the 7,12-Dimethylbenz[a]anthracene (DMBA)-Induced Hamster Buccal Pouch Model of Oral Dysplasia and Squamous Cell Carcinoma

Abstract

Purpose Currently, 2-deoxy-2-[18F]fluoro-d-glucose (18F-FDG) is the gold standard radiotracer for staging of head and neck cancer; however, the low sensitivity of this tracer can impede detection of early lesions. 64Cu-liposomes accumulate in various cancers and provide both a sensitive tracer and an indication of the biodistribution of nanotherapeutics. Here, the accumulation of 64Cu-liposomes in early and established cancers is assessed and compared with 18F-FDG in a head and neck cancer model. Methods Lesions ranging from mild dysplasia to squamous cell carcinoma were induced in a hamster model of head and neck cancer by topical application of 7,12-dimethylbenz[a]anthracene to the buccal pouch. The hamsters were imaged with micro-positron emission tomography using 18F-FDG and 64Cu-liposomes. Results At 24 h postinjection, 64Cu-liposome accumulation exceeded the accumulation of 18F-FDG in every pathologic grade. The lesion-to-cheek pouch (background) ratio and lesion-to-brain ratio were also higher for 64Cu-liposomes than for 18F-FDG. Conclusion Imaging of a nanotracer such as 64Cu-liposomes can improve the visualization of head and neck tumors. Accumulation of liposomal particles in head and neck tumors over various pathologic grades averaged 3.5 %ID/cc demonstrating the potential for liposomal therapy with targeted chemotherapeutic agents.

<p>Noninvasive Molecular Imaging of the Enhanced Permeability and Retention Effect by ⁶⁴Cu-Liposomes: In vivo Correlations with ⁶⁸Ga-RGD, Fluid Pressure, Diffusivity and ¹⁸F-FDG</p>

“…No previous publications have correlated FDG uptake and liposome accumulation, but two publications with a different research focus have co-imaged experimental tumors with 64 Cu-liposome and 18 F-FDG PET/CT. 46 , 47 …”

Regulation of Abnormal Expression of hsa_circRNA_0127523&nbsp;on Proliferation, Migration and Invasion and mRNA&nbsp;Expression of miR-515-5p&nbsp;in Oral Squamous Cell Carcinoma

“…They were divided into three groups: control group (n=30), negative control group (n=12) and experimental (model) group (n=48). The control group was not any treated, the negative control group was coated with acetone and the model group was coated with acetone-dissolved 9, 10-Dimethy1-1, 2-Benzanthracence (DMBA) [20]. Animal experiments were performed at the Experimental Animal Center of Shanxi Medical University and carried out strictly in accordance with the operating rules formulated by the Institutional Animal Care and Use Committee of Shanxi Medical University (IACUC 2017-016).…”

Future trends and emerging issues for nanodelivery systems in oral and oropharyngeal cancer

“…They were able to determine superior accumulation of the liposomes over the standard radiotracer, demonstrating a potential use for liposomal therapy together with chemotherapeutics. 67 By Atto-488 labeling of micelles containing elastin-like polypeptide, gastrin-releasing peptide, and a hydrophobic drug, the endocytosis of NPs was tracked through confocal imaging. 68 With their high electron density, gold nanoparticles (GNPs) offer superior contrast in TEM images.…”

“…As a radiotracer, 64 Cu liposomes are able to augment HNSCC visualization and early detection. 67 Boron neutron-capture therapy is a dual cancer-therapy option that combines selective buildup of tumor-targeting compounds containing boron and neutron irradiation. Consequently, tumor tissue is selectively targeted for irradiation.…”