Comparison of phenolic extracts obtained of Pinus radiata bark from pulp and paper industry and sawmill industry

Abstract

Pinus radiata barks obtained from tress of different ages, as subproduct of pulp and paper (trees less than 10 years) and sawmill (trees between 20 to 25 years) industries, were used to produce extracts containing phenolic compounds. A factorial design 2 3 was used to evaluate the influence of the variables temperature (25 -35 ºC), solvent type (acetone -ethanol) and extraction time (1-12 h). The extracts were compared in their extraction yield (%), total phenols (by FolinCiocalteau), and radical scavenging activity (by DPPH). The extract obtained from old trunks presented a higher extraction yield than from young trees. The highest yield value was 2.56%, which was obtained using acetone as solvent for 12 h and 35°C. The highest concentration of phenol (5.84±0.18 g CE g extract -1 ), and scavenging activity (IP=86.1±4.4%) were also obtained for this type of extract. The extraction duration was the variable that most influenced the parameters studied. The bark's radical scavenging power was greater than BHT (40%) and slightly lower than ascorbic acid (92%), common commercial antioxidants.

Screening of Wood/Forest and Vine By-Products as Sources of New Drugs for Sustainable Strategies to Control Fusarium graminearum and the Production of Mycotoxins

“…In the present study, the antioxidant activity of the extracts was positively correlated with total phenolic contents and seemed to be influenced by phenolic composition. The same trend has frequently been reported in previous studies focusing on natural extracts [ 24 , 25 ]. The high levels of antioxidant activity of chestnut chip 175 °C, maritime pine bark 125 °C, and maritime pine bark 175 °C extracts could be related to their phenolic composition.…”

Antioxidant Activity of Leaves and Fruits of Cultivated Conifers in Iran

“…There are several reports on the evaluation of antioxidant activities of extracts and essential oils of Pinus species mainly P. halepensis using different antioxidant activity tests. Results indicated that the extracts and essential oils of these plants usually showed a strong activity in these antioxidant assays (28-39). But there is no report on evaluation of antioxidant effect of the methanol extracts obtained from leaves and fruits of Iranian common conifers.…”

“…Several reports have been published on evaluation of the antioxidant activity of the extract of various species of conifers (28-39). In the current study the antioxidant activity of the methanol extract (final concentration 0.02% w/v) of leaves and fruits of Iranian common conifers (six species) were investigated using two different antioxidant assays; Ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods (41).…”

“…Several researches have been done regarding antioxidant activity of various species of conifers in order to investigate the antioxidant activity from natural sources as alternatives of commercial antioxidants (28-39). However there are no published reports on antioxidant properties of the crude extract (methanol) obtained from leaves and fruits of Iranian common conifers.…”

Application of aqueous two phase systems based on polyethylene glycol and sodium citrate for the recovery of phenolic compounds from Eucalyptus wood

“…Extraction is a common method for the isolation of natural antioxidants and it is mainly performed with organic solvents (Vázquez et al 2008, Aspé and Fernández 2011, Fernández-Agulló et al 2013, Ramos et al 2013. Although this procedure is efficient and solvents such as ethanol are GRAS (generally recognized as safe), the extracts from other solvents are generally not safe for human consumption due to potentially toxic effects of residual solvents (Salic et al 2011).…”