Comparison of phospholipid composition of<i>Aedes aegypti</i> and<i>Aedes albopictus</i> cells obtained from logarithmic and stationary phases of growth

Jenkin, Howard M.; McMeans, E.; Anderson, L. E.; Yang, Tao

doi:10.1007/bf02532762

Cited by 17 publications

(11 citation statements)

References 13 publications

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“…This distribution is similar to the membrane lipid composition of eukaryotic cells where the most abundant phospholipid is PC [21]. It is also consistent with the membrane lipid composition of the Diptera species where PE is a predominant PL [22]. CER-PE, which is preferentially expressed in insect cells, is also observed here [23][24].…”

Section: Author Summarysupporting

confidence: 88%

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Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells

et al. 2012

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Dengue virus causes ∼50–100 million infections per year and thus is considered one of the most aggressive arthropod-borne human pathogen worldwide. During its replication, dengue virus induces dramatic alterations in the intracellular membranes of infected cells. This phenomenon is observed both in human and vector-derived cells. Using high-resolution mass spectrometry of mosquito cells, we show that this membrane remodeling is directly linked to a unique lipid repertoire induced by dengue virus infection. Specifically, 15% of the metabolites detected were significantly different between DENV infected and uninfected cells while 85% of the metabolites detected were significantly different in isolated replication complex membranes. Furthermore, we demonstrate that intracellular lipid redistribution induced by the inhibition of fatty acid synthase, the rate-limiting enzyme in lipid biosynthesis, is sufficient for cell survival but is inhibitory to dengue virus replication. Lipids that have the capacity to destabilize and change the curvature of membranes as well as lipids that change the permeability of membranes are enriched in dengue virus infected cells. Several sphingolipids and other bioactive signaling molecules that are involved in controlling membrane fusion, fission, and trafficking as well as molecules that influence cytoskeletal reorganization are also up regulated during dengue infection. These observations shed light on the emerging role of lipids in shaping the membrane and protein environments during viral infections and suggest membrane-organizing principles that may influence virus-induced intracellular membrane architecture.

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Section: Author Summarysupporting

confidence: 88%

“…There was also limited overlap between the specific PE molecular species regulated between DENV-and UV-DENV-exposed cells (Table S1). Given that insect cells have a high abundance of PE in their membranes (40-50%), it is interesting that there is a selective requirement for PC (over PE) in DENV-infected mosquito cells [22].…”

Section: Author Summarymentioning

confidence: 99%

Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells

et al. 2012

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“…Similarity in enzyme subcellular localizations, membrane and endomembrane compositions also suggest a certain homology in lipid biosyntheses ( Butters and Hughes, 1981 ). Aedes mosquito cells contain an abundance of PC and PE and the same PL categories as in mammals ( Townsend et al, 1972 ; Jenkin et al, 1975 ). However, while PC is the main PL in mammalian cells, Aedes mosquito cells contain a majority of PE ( Luukkonen et al, 1973 ; Jenkin et al, 1975 ; Dawaliby et al, 2016 ), and other substantial differences in lipid metabolism between insects and mammals exist ( Canavoso et al, 2001 ).…”

Section: Flaviviruses Subdue Phospholipids In Mosquitoesmentioning

confidence: 99%

Lipid Interactions Between Flaviviruses and Mosquito Vectors

et al. 2021

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Mosquito-borne flaviviruses, such as dengue (DENV), Zika (ZIKV), yellow fever (YFV), West Nile (WNV), and Japanese encephalitis (JEV) viruses, threaten a large part of the human populations. In absence of therapeutics and effective vaccines against each flaviviruses, targeting viral metabolic requirements in mosquitoes may hold the key to new intervention strategies. Development of metabolomics in the last decade opened a new field of research: mosquito metabolomics. It is now clear that flaviviruses rely on mosquito lipids, especially phospholipids, for their cellular cycle and propagation. Here, we review the biosyntheses of, biochemical properties of and flaviviral interactions with mosquito phospholipids. Phospholipids are structural lipids with a polar headgroup and apolar acyl chains, enabling the formation of lipid bilayer that form plasma- and endomembranes. Phospholipids are mostly synthesized through the de novo pathway and remodeling cycle. Variations in headgroup and acyl chains influence phospholipid physicochemical properties and consequently the membrane behavior. Flaviviruses interact with cellular membranes at every step of their cellular cycle. Recent evidence demonstrates that flaviviruses reconfigure the phospholipidome in mosquitoes by regulating phospholipid syntheses to increase virus multiplication. Identifying the phospholipids involved and understanding how flaviviruses regulate these in mosquitoes is required to design new interventions.

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“…We examined the EM map in the vicinity of the pocket and found that it is occupied by strong density resembling the headgroup of a phosphatidylethanolamine lipid (PE), with partially resolved fatty acid tails (Fig 4b, c). PE is the most abundant phospholipid in the lipidome of Aedes aegypti and Aedes albopictus cells 31 .…”

Section: Rearrangement Of Amphipathic Helices Leads To Formation Of Amentioning

confidence: 99%

A high resolution view of an adolescent flavivirus

Renner

Dejnirattisai

Carrique

et al. 2020

Preprint

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Mosquito-transmitted flaviviruses, such as Dengue virus (DENV) or Zika virus (ZIKV), are responsible for significant economic damage and human misery. In infected cells, flaviviruses first assemble into an immature form within the endoplasmatic reticulum (ER), and then undergo further processing by furin protease in the trans-Golgi. Despite substantial efforts, previous cryogenic electron microscopy (cryo-EM) studies of immature flaviviruses were restricted to low to medium resolutions, limiting our understanding of maturation. To better grasp the process of maturation, we have carried out cryo-EM reconstructions of immature Spondweni virus (SPOV), an emerging human flavivirus belonging to the same serogroup as ZIKV (~75% amino acid identity). By combining localized reconstruction and focused refinement, we were able to improve the resolution to 3.8 Å, yielding unprecedented insight into the immature form. The structure elucidates how, at neutral pH, polar interactions conceal the furin recognition site within trimeric envelope (E) protein spikes. Furthermore, we identify how a strictly conserved pH sensor anchors the precursor membrane (prM) protein to immature E. We reconstructed mature forms of SPONV and DENV to 2.6Å and 3.1Å, respectively. Comparison with immature virus shows a conserved binding pocket for a lipid headgroup, which forms as a consequence of the rearrangement of amphipathic stem-helices of E. We propose a structural role for the pocket and suggest it stabilizes mature E. Taken together, our data suggest a compelling rationale for low-pH triggered conformational rearrangement in the Golgi, which occurs during flavivirus maturation. especially D. Clare, for assistance with data collection and advice on data processing. We also thank L. Mendonça, T. Frosio, and members of the P. Zhang group for scientific discussions.

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Comparison of phospholipid composition ofAedes aegypti andAedes albopictus cells obtained from logarithmic and stationary phases of growth

Cited by 17 publications

References 13 publications

Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells

Dengue Virus Infection Perturbs Lipid Homeostasis in Infected Mosquito Cells

Lipid Interactions Between Flaviviruses and Mosquito Vectors

A high resolution view of an adolescent flavivirus

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