No abstract

The total lipids, total neutral lipids, and total phospholipids from Aedes aegypti and aedes albopictus cells cultivated in vitro in a medium containing fetal calf serum were analyzed. The mosquito cells were harvested in the logarithmic and stationary phases of growth. The fatty acid profiles of the lipids showed differences during the aging of the cells but not betweeen species. There was an increase in chain elongation and unsaturation of the fatty acids in the stationary phase when compared with the logarithmic phase of growth. The major components of the fatty acid profiles of the cells were 16:0, 16:1, and 18:1 fatty acids. Few similarities were found between the lipid analysis of the mosquito cells and the growth medium.

Aedes aegypti and Aedes albopictus cells were grown in tissue culture and harvested at logarithmic and stationary phases of development. The phospholipids were extracted, separated into lipid classes, and fatty acid composition of each fraction determined. The phosphatidylethanolamine fraction was the major lipid (42-54%). With aging, the A. aegypti cells showed an increase in polyenes in the phosphatidylcholine and phosphatidylethanolamine fractions and in monoenes and polyenes in the phosphatidylinositol fraction. The lysophosphatidylcholine fraction had an increase in chain length of the fatty acids with aging of the A. aegypti cells. The A. albopictus cells, with aging, showed increases in chain length and in the relative percentage of polyenes in the lysophosphatidylcholine, phosphatidylcholine, and phosphatidylserine fractions. In the phosphatidylinositol fraction, chain elongation of fatty acids occurred as the cells aged. In the ceramide phosphorylcholine fraction, there were increases in saturation and chain elongation of the fatty acids from the logarithmic to the stationary phase of the A. albopictus cells. An increase in polyenes was observed with aging of the cells in the phosphatidylethanolamine fraction.