Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization

Aragón, C.; Lara, Claudia; González-Olmedo, J. L.; Escalona, Maritza; Carvalho, Luísa C.; Amâncio, Sara

doi:10.1007/s10535-013-0381-6

Cited by 47 publications

(38 citation statements)

References 45 publications

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“…The advantage of SABs is that they increase multiplication rates, allow greater availability of culture medium components and reduce production costs [ 7 , 8 , 9 ]. Micropropagation in SABs promotes photosynthesis and respiration, both caused by an increase in chlorophyll synthesis and stomatal functionality [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

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Section: Introductionmentioning

confidence: 99%

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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“…plants from TIB cultures had a higher survival rate than those from gelled medium. Photosynthetic rate at the midpoint of acclimatized plants grown in TIB cultures was significantly higher than that of plants grown in gelled media (Aragon et al 2014). Chlorophyll content of leaves is indirectly related to nitrogen content, and carotenoids increase antioxidant activity with leaf growth (Zhao et al 2005).…”

Section: Discussionmentioning

confidence: 95%

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

An¹,

Kim²,

Moon³

et al. 2016

J Plant Biotechnol

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We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.

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“…Many previous reports indicated that plantlets from TIS had greater growth, with rapid adaptation after exposure to ex vitro conditions than SSS and CIS derived plant [18,33]. This might be because TIS enhanced stomatal functioning and improved photosynthesis and transpiration [31,34]. However, E. flava plantlets visibly wilted during transfer to ex vitro acclimatization.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

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Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

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Comparison of plantain plantlets propagated in temporary immersion bioreactors and gelled medium during in vitro growth and acclimatization

Cited by 47 publications

References 45 publications

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

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